發(fā)布時(shí)間：2018-06-20 12:08

  本文選題：錳中毒 + 海馬��； 參考：《環(huán)境與職業(yè)醫(yī)學(xué)》2017年08期

【摘要】：[目的]探討錳中毒大鼠海馬區(qū)錳和鋅含量變化及與鋅轉(zhuǎn)運(yùn)蛋白3(ZnT3)之間的關(guān)系。[方法]將40只雄性SD大鼠隨機(jī)分成4組,每組10只,即生理鹽水對(duì)照組和染錳低劑量、中劑量、高劑量組。分別給予腹腔注射生理鹽水及氯化錳溶液3、6、12 mg/(kg·d)連續(xù)注射8周,錳中毒造模成功后取大鼠海馬組織,做HE染色病理切片觀察其病理改變,石墨爐原子吸收儀測定海馬錳、鋅含量變化,Western blot法檢測大鼠海馬區(qū)ZnT3含量,實(shí)時(shí)熒光定量PCR法測定大鼠海馬區(qū)ZnT3 mRNA的表達(dá)。[結(jié)果]染毒后大鼠海馬神經(jīng)細(xì)胞均出現(xiàn)不同程度嗜酸性變,胞漿凝聚、結(jié)構(gòu)不清,且出現(xiàn)細(xì)胞皺縮和嗜酸性小體。與對(duì)照組及低劑量組比較,高、中劑量組大鼠海馬組織中錳及鋅含量均明顯增高(P0.05);而高劑量組鋅含量也高于中劑量組(P0.01)。高、中、低劑量組大鼠海馬組織中ZnT3含量分別為1.01±0.03、0.80±0.02、0.65±0.13,均高于對(duì)照組(0.51±0.06)(P0.05)。高、中劑量組ZnT3基因相對(duì)表達(dá)分別為3.40±0.36、2.88±0.69,均高于對(duì)照組及低劑量組(1.47±0.60)(P0.001)。[結(jié)論]錳中毒可破壞大鼠海馬神經(jīng)元細(xì)胞結(jié)構(gòu),且海馬組織中鋅含量與ZnT3同步增高。
[Abstract]:[objective] to investigate the changes of manganese and zinc content in hippocampus of rats with manganese poisoning and their relationship with zinc transporter 3 ZnT 3. [methods] Forty male Sprague-Dawley rats were randomly divided into 4 groups, 10 rats in each group: saline control group, low dose, middle dose and high dose group. Rats were injected intraperitoneally with normal saline and manganese chloride solution for 8 weeks. The hippocampal tissues of rats were taken out after the model was established successfully. The pathological changes were observed by HE staining, and the hippocampal manganese was determined by graphite furnace atomic absorption spectrometry (GFAAS). The content of ZnT3 in hippocampus of rats was detected by Western blot and the expression of ZnT3 mRNA in hippocampus of rats was detected by real-time fluorescence quantitative polymerase chain reaction. [results] all the hippocampal neurons showed eosinophilic changes, cytoplasmic condensation, unclear structure, cell shrinkage and eosinophilic bodies. Compared with the control group and the low dose group, the content of mn and Zn in the hippocampus of the middle dose group was significantly higher than that of the control group and the low dose group, while the content of zinc in the high dose group was higher than that in the middle dose group, and the content of zinc in the middle dose group was also higher than that in the middle dose group. The contents of ZnT3 in hippocampus of rats in high, medium and low dose groups were 1.01 鹵0.03, 0.80 鹵0.02 and 0.65 鹵0.13, respectively, which were higher than those in control group (0.51 鹵0.06). The relative expression of ZnT3 gene in the high and middle dose groups was 3.40 鹵0.36 ~ 2.88 鹵0.69, respectively, which was higher than that in the control group and low dose group (1.47 鹵0.60). [conclusion] Manganese poisoning can destroy the structure of hippocampal neurons in rats, and the content of zinc in hippocampal tissue increases synchronously with ZnT3.
【作者單位】： 遵義醫(yī)學(xué)院公共衛(wèi)生學(xué)院衛(wèi)生毒理學(xué)教研室;
【基金】：貴州省科學(xué)技術(shù)廳國際科技合作項(xiàng)目(編號(hào):黔科合外G字[2014]7012號(hào))
【分類號(hào)】：R595.2

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