本文選題：骨質(zhì)疏松 + 絕經(jīng)后�。� 參考：《中國(guó)組織工程研究》2017年24期

【摘要】：背景:絕經(jīng)期后骨質(zhì)疏松癥與牙周組織疾病有著十分密切的關(guān)系,而其作用機(jī)制尚不清楚。目的:通過(guò)切除卵巢建立雌激素缺乏狀態(tài)下骨質(zhì)疏松小鼠模型,觀察在此狀態(tài)下牙槽骨組織中核轉(zhuǎn)錄因子κB以及血清與牙齦組織中白細(xì)胞介素17的表達(dá)變化。方法:將3月齡雌性C3H小鼠隨機(jī)分為卵巢切除組及假手術(shù)組。術(shù)后10周,蘇木精-伊紅染色觀察下頜骨及股骨組織學(xué)改變;RT-PCR檢測(cè)下頜骨中成骨相關(guān)因子OCN及Runx2表達(dá)變化;免疫組化染色及Western Blot檢測(cè)下頜骨中核轉(zhuǎn)錄因子κB表達(dá);CBA流式液相多重蛋白定量技術(shù)檢測(cè)血清及牙齦組織勻漿中白細(xì)胞介素17水平。結(jié)果與結(jié)論:(1)卵巢切除組小鼠股骨的骨皮質(zhì)變薄,骨髓腔擴(kuò)大,骨小梁變細(xì)、數(shù)目減少、密度降低、結(jié)構(gòu)不完整,骨吸收陷窩樣結(jié)構(gòu)增多;(2)卵巢切除組牙槽骨組織中OCN及Runx2相對(duì)表達(dá)量較假手術(shù)組低;(3)卵巢切除組小鼠下頜骨中核轉(zhuǎn)錄因子κB活化形式P65蛋白呈陽(yáng)性表達(dá);(4)卵巢切除組牙槽骨組織中核轉(zhuǎn)錄因子κB活化形式P65相對(duì)表達(dá)量高于假手術(shù)組;(5)卵巢切除組小鼠血清中白細(xì)胞介素17表達(dá)水平高于假手術(shù)組,但牙齦組織中白細(xì)胞介素17表達(dá)水平與假手術(shù)組相比無(wú)明顯變化;(6)實(shí)驗(yàn)表明,雌激素缺乏導(dǎo)致的核轉(zhuǎn)錄因子κB信號(hào)通路激活可能在牙周骨吸收中發(fā)揮一定作用;而牙周局部組織內(nèi)的白細(xì)胞介素17可能并非此狀態(tài)下牙周組織破壞的關(guān)鍵因子。
[Abstract]:Background: postmenopausal osteoporosis has a close relationship with periodontal tissue disease, but its mechanism is unclear. Aim: to establish an ovariectomized mouse model of osteoporosis with estrogen deficiency and to observe the changes of NF- 魏 B in alveolar bone and the expression of interleukin-17 in serum and gingival tissue. Methods: 3 month old female C 3 H mice were randomly divided into ovariectomized group and sham operation group. The histological changes of mandible and femur were observed by hematoxylin and eosin staining 10 weeks after operation. The expression of OCN and Runx2 in mandible was detected by RT-PCR. Immunohistochemical staining and Western blot were used to detect the expression of nuclear factor 魏 B (NF- 魏 B) in mandible. The levels of interleukin 17 in serum and gingival homogenate were detected by CBA flow multiplex protein quantification technique. Results and conclusion the femur of the ovariectomized mice was thinned, the medullary cavity was enlarged, the bone trabeculae were thinned, the number of bone trabeculae was decreased, the density was decreased, and the structure of the femur was incomplete. The relative expression of OCN and Runx2 in alveolar bone in ovariectomized group was lower than that in sham-operated group. The relative expression of p65 in alveolar bone tissue was higher than that in sham operation group (P < 0.05). The expression of interleukin 17 in serum of ovariectomized mice was higher than that of sham operation group. However, the level of interleukin 17 expression in gingival tissue was not significantly changed compared with that in sham operation group. The results showed that activation of transcription factor 魏 B signaling pathway induced by estrogen deficiency may play a role in periodontal bone resorption. Interleukin-17 in periodontal tissue may not be the key factor of periodontal tissue destruction in this condition.
【作者單位】： 天津醫(yī)科大學(xué)口腔醫(yī)院牙體牙髓科;
【基金】：天津市自然科學(xué)基金一般項(xiàng)目(15JCYBJC50200)和重點(diǎn)項(xiàng)目(16JCZDJC32800)~~
【分類號(hào)】：R580;R781.4

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