本文選題：丁苯酞膠囊 + 一氧化碳中毒��； 參考：《中國(guó)臨床藥理學(xué)雜志》2017年08期

【摘要】：目的研究丁苯酞對(duì)大鼠急性重度CO中毒后腦組織中半胱氨酸蛋白-3(Caspase-3)和半胱氨酸蛋白酶-9(Caspase-9)表達(dá)的影響。方法通過(guò)數(shù)字表法將大鼠隨機(jī)分為3組:正常組、模型組和實(shí)驗(yàn)組(均n=10)。用CO染毒法制作大鼠急性重度CO中毒模型。實(shí)驗(yàn)組大鼠給予丁苯酞80 mg·kg~(-1),灌胃1次/天,連續(xù)7 d,正常組和模型組給予等量的0.9%NaCl。在7 d后,用免疫組化染色法觀測(cè)Caspase-3和Caspase-9陽(yáng)性細(xì)胞數(shù),用反轉(zhuǎn)錄酶-聚合酶鏈鎖反應(yīng)(RT-PCR)法及蛋白質(zhì)印跡法檢測(cè)Caspase-3和Caspase-9mRNA和蛋白的表達(dá)水平。結(jié)果正常組大鼠腦組織中Caspase-3和Caspase-9的陽(yáng)性細(xì)胞數(shù)分別為4.98±1.34,8.65±1.89,mRNA表達(dá)水平分別為0.19±0.02,0.18±0.03,蛋白表達(dá)水平分別為0.09±0.01,0.08±0.01;模型組大鼠腦組織中Caspase-3和Caspase-9陽(yáng)性細(xì)胞數(shù)分別為29.43±4.53,40.45±5.23,mRNA表達(dá)水平分別為0.47±0.05,0.75±0.05,蛋白表達(dá)水平分別為0.78±0.05,0.86±0.07。與正常組對(duì)比,模型組的Caspase-3和Caspase-9mRNA和蛋白表達(dá)水平顯著增高,差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。實(shí)驗(yàn)組大鼠腦組織中的Caspase-3和Caspase-9陽(yáng)性細(xì)胞數(shù)分別為22.26±3.12,25.09±4.25,mRNA表達(dá)水平分別為0.35±0.05,0.41±0.05,蛋白表達(dá)水平分別為0.37±0.02,0.45±0.02,與模型組對(duì)比,Caspase-3和Caspase-9 mRNA和蛋白表達(dá)水平較明顯下調(diào),差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。結(jié)論丁苯酞可通過(guò)下調(diào)Caspase-3和Caspase-9的表達(dá)水平來(lái)抑制神經(jīng)細(xì)胞凋亡進(jìn)程,從而減輕CO對(duì)大鼠腦神經(jīng)的損害。
[Abstract]:Objective to study the effects of butyphthalide on the expression of cysteine protein-3 (caspase-3) and cysteine protease-9 (Caspase-9) in brain tissue of rats with acute severe CO poisoning. Methods the rats were randomly divided into 3 groups: normal group, model group and experimental group (n = 10). The acute severe CO poisoning model of rats was made by means of CO poisoning. Rats in the experimental group were given butylphthalide (80 mg 路kg ~ (-1) by gavage once a day for 7 days. The normal group and the model group were given the same amount of 0.9 NaCl. After 7 days, the number of Caspase-3 and Caspase-9 positive cells were detected by immunohistochemical staining, and the expression levels of Caspase-3 and Caspase-9 mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. Results the number of Caspase-3 and Caspase-9 positive cells in the brain tissue of normal rats was 4.98 鹵1.34, 8.65 鹵1.89, respectively, 0.19 鹵0.02 鹵0.18 鹵0.03, and 0.09 鹵0.01 鹵0.08 鹵0.01, respectively, and the number of Caspase-3 and Caspase-9 positive cells in brain tissue of model group was 29.43 鹵4.53, 40.45 鹵5.23, respectively. The expression level of protein was 0.78 鹵0.05 and 0.86 鹵0.07, respectively. Compared with the normal group, the expression of Caspase-3 and Caspase-9 mRNA and protein in the model group was significantly higher than that in the normal group (all P 0.05). The number of Caspase-3 and Caspase-9 positive cells in brain tissue of experimental group was 22.26 鹵3.12n25.09 鹵4.25, respectively. The expression levels of Caspase-3 and Caspase-9 mRNA and protein were 0.35 鹵0.05, 0.41 鹵0.05, 0.37 鹵0.02, 0.45 鹵0.02, respectively. Compared with the model group, the expression levels of Caspase-3 and Caspase-9 mRNA and protein were significantly down-regulated (P 0.05). Conclusion butyphthalide can inhibit the process of neuronal apoptosis by down-regulating the expression levels of Caspase-3 and Caspase-9, thereby reducing the damage of CO to the brain nerve of rats.
【作者單位】： 福建醫(yī)科大學(xué)附屬泉州第一醫(yī)院神經(jīng)內(nèi)科;
【基金】：泉州市衛(wèi)生科研基金資助項(xiàng)目
【分類號(hào)】：R595.1;R747.9

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