本文選題：gp + Tregs��； 參考：《生物工程學(xué)報(bào)》2016年12期

【摘要】：旨在以非肥胖糖尿病(Non-obese diabetic,NOD)小鼠為動物模型,研究高劑量昆蟲細(xì)胞表達(dá)的重組熱休克蛋白gp96(Recombinant gp96,rgp96)對1型糖尿病(Type 1 diabetes,T1D)的預(yù)防作用。以高劑量rgp96免疫NOD小鼠,用血糖儀監(jiān)測小鼠血糖值,用流式細(xì)胞術(shù)檢測小鼠脾臟CD4~+CD25~+Foxp3~+調(diào)節(jié)性T細(xì)胞(Regulatory T cells,Tregs)亞群頻率,然后用一系列體外實(shí)驗(yàn)探究高劑量rgp96對Tregs的影響。結(jié)果顯示高劑量rgp96免疫有效地預(yù)防或延緩小鼠T1D發(fā)病,免疫誘導(dǎo)Tregs數(shù)量明顯增加。體外實(shí)驗(yàn)發(fā)現(xiàn)rgp96蛋白促進(jìn)Tregs增殖,誘導(dǎo)Foxp3表達(dá)上調(diào)和IL-10分泌增加。研究結(jié)果為開發(fā)基于rgp96的新型T1D預(yù)防和治療性疫苗提供了依據(jù)。
[Abstract]:To study the preventive effect of recombinant heat shock protein gp96 (Recombinant gp96 rgp96) on type 1 diabetes mellitus (T1D) mice with non-obesity-related diabetes mellitus (NOD) as an animal model, and to study the preventive effects of recombinant heat shock protein gp96 / Recombinant gp96 / rgp96 on type 1 diabetes mellitus (type 1 diabetes). High dose rgp96 was used to immunize nod mice. Blood glucose levels were monitored by blood glucose analyzer. The frequency of T cells Tregs subsets in spleen of mice was detected by flow cytometry. The effects of high dose rgp96 on Tregs were investigated by a series of experiments in vitro. The results showed that high dose of rgp96 could effectively prevent or delay the onset of T1D in mice and increase the number of Tregs induced by T1D. It was found in vitro that rgp96 protein promoted the proliferation of Tregs and induced the up-regulation of Foxp3 expression and the increase of IL-10 secretion. The results provide a basis for the development of a novel T 1D preventive and therapeutic vaccine based on rgp96.
【作者單位】： 中國科學(xué)院微生物研究所中國科學(xué)院病原微生物與免疫學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(Nos.31230026,81321063,81471960) 深圳市科技創(chuàng)新委員會項(xiàng)目(Nos.JSGG20140516112337659,CYZZ20130826112642412)資助~~
【分類號】：R587.1
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本文編號：2001484