本文選題：有氧運(yùn)動(dòng) + 2型糖尿病��； 參考：《河北師范大學(xué)》2017年碩士論文

【摘要】：研究目的:目前,我國以及全球的2型糖尿病(T2DM)患病人數(shù)不斷增加,引起了各界研究學(xué)者的普遍關(guān)注,由于T2DM很難治愈,因此尋找預(yù)防T2DM的方法顯得尤為重要。規(guī)律的運(yùn)動(dòng)是促進(jìn)代謝適應(yīng),防治多種代謝性疾病的有效手段。一氧化氮(NO)是一個(gè)無機(jī)分子,在細(xì)胞間充當(dāng)信號(hào)分子,在生理學(xué)、生物化學(xué)、生物醫(yī)學(xué)領(lǐng)域被廣泛研究。沉默交配型信息調(diào)節(jié)因子2同源蛋白1(SIRT1)參與了葡萄糖/脂質(zhì)代謝、線粒體生物合成等生理過程。最近有研究表明,運(yùn)動(dòng)可以通過NO/SIRT1介導(dǎo)的調(diào)控通路來改善T2DM患者的骨骼肌胰島素敏感性,但在預(yù)防T2DM過程中,此通路的作用鮮有報(bào)道。本實(shí)驗(yàn)通過觀察有氧運(yùn)動(dòng)干預(yù)后,高糖高脂膳食小鼠腓腸肌中一氧化氮合酶(NOS)活性、NO濃度和SIRT1蛋白表達(dá)變化,明確有氧運(yùn)動(dòng)刺激小鼠腓腸肌NOS/NO和SIRT1有調(diào)節(jié)空腹血糖、調(diào)節(jié)胰島素敏感性和預(yù)防T2DM的重要作用,為預(yù)防T2DM提供新思路。研究方法:8周齡雄性Balb/Cj品系小鼠24只,隨機(jī)分為:安靜普通飼料組(CC組)、有氧運(yùn)動(dòng)普通飼料組(EC組)、高脂高糖膳食安靜組(HC組)、高脂高糖膳食有氧運(yùn)動(dòng)組(HE組),每組6只。EC組和HE組的小鼠進(jìn)行為期6周的跑臺(tái)實(shí)驗(yàn),速度從10m/min遞增到15m/min,每一周遞增一次速度(1m/min),每周5次,每次運(yùn)動(dòng)時(shí)間為45min,坡度為0。CC組和EC組進(jìn)行普通飼料喂養(yǎng),HC組和HE組進(jìn)行高糖高脂膳食喂養(yǎng)。實(shí)驗(yàn)動(dòng)物處死前,用羅氏血糖儀檢測空腹血糖(FBG)水平,處死取材后,ELISA法檢測空腹胰島素(FIN)含量并計(jì)算出胰島素敏感指數(shù)(ISI)和胰島素抵抗指數(shù)(IR),采用生化試劑盒檢測小鼠腓腸肌NOS活力、NO含量,Western blot法測定小鼠腓腸肌SIRT1蛋白表達(dá)。所得數(shù)據(jù)用SPSS20.0軟件進(jìn)行雙因素方差分析。研究結(jié)果:(1)6周干預(yù)后,HC組小鼠的FBG水平和FIN含量均高于CC組,HE組、EC組分別均低于HC組、CC組,有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠FBG水平的影響均顯著(P0.01),對(duì)小鼠FIN含量的影響均顯著(P0.01,P0.05);HC組小鼠的ISI低于CC組,HE組、EC組分別高于HC組、CC組,有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠ISI的影響均顯著(P0.01);HC組小鼠的IR高于CC組,HE組、EC組分別低于HC組、CC組,有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠IR的影響均顯著(P0.01)。(2)有氧運(yùn)動(dòng)和膳食干預(yù)對(duì)小鼠腓腸肌tNOS活力、iNOS活力的交互作用影響均顯著(P0.01);HE組小鼠腓腸肌tNOS活力、iNOS活力均低于HC組(P0.01),EC組tNOS活力高于CC組,EC組iNOS活力與CC組無差異;有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠腓腸肌中cNOS活力影響均顯著(P0.01),HE組小鼠腓腸肌中cNOS活力高于HC組,EC組高于CC組。(3)有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠腓腸肌NO含量的交互作用影響顯著(P0.05);6周后,HE組低于HC組,有氧運(yùn)動(dòng)對(duì)高糖高脂膳食小鼠腓腸肌NO含量的影響顯著(P0.05),對(duì)普通膳食小鼠無影響。(4)有氧運(yùn)動(dòng)和高糖高脂膳食干預(yù)對(duì)小鼠腓腸肌SIRT1蛋白表達(dá)的交互作用影響顯著(P0.05),HE組小鼠腓腸肌SIRT1蛋白表達(dá)高于HC組,有氧運(yùn)動(dòng)對(duì)高糖高脂膳食小鼠腓腸肌SIRT1蛋白表達(dá)影響顯著(P0.01),對(duì)普通膳食小鼠無影響。研究結(jié)論:(1)有氧運(yùn)動(dòng)降低了高糖高脂膳食小鼠的空腹血糖水平和空腹胰島素含量,提高了高糖高脂膳食小鼠的胰島素敏感性,降低了胰島素抵抗。(2)有氧運(yùn)動(dòng)降低了高糖高脂膳食小鼠腓腸肌的tNOS活性,有助于緩解高糖高脂膳食小鼠的NO含量大量增加。(3)有氧運(yùn)動(dòng)顯著提高了高糖高脂膳食小鼠腓腸肌SIRT1蛋白表達(dá)。
[Abstract]:Research purposes: at present, the number of patients with type 2 diabetes (T2DM) in China and the world is increasing, which has attracted the attention of researchers from all walks of life. Because T2DM is difficult to cure, it is very important to find a way to prevent T2DM. Regular exercise is an effective means to promote metabolic adaptation and prevent many kinds of metabolic diseases. Nitric oxide (NO) It is an inorganic molecule that acts as a signal molecule between cells and is widely studied in the field of physiology, biochemistry and biomedicine. The silent mating type information regulator 2 homologous protein 1 (SIRT1) participates in the physiological processes such as glucose / lipid metabolism and mitochondrial biosynthesis. The most recent studies show that exercise can be regulated by NO/SIRT1 mediated regulation. The pathway is used to improve the insulin sensitivity of skeletal muscle in T2DM patients, but in the process of preventing T2DM, the role of this pathway is rarely reported. In this experiment, the activity of nitric oxide synthase (NOS) in the gastrocnemius muscle of high glucose and high fat diet mice, the concentration of NO and the expression of SIRT1 egg white in the high fat and high fat diet mice were observed, and the NOS of the gastrocnemius muscle of mice was clearly stimulated by the aerobic exercise. /NO and SIRT1 have an important role in regulating fasting blood glucose, regulating insulin sensitivity and preventing T2DM, and providing new ideas for the prevention of T2DM. Methods: 8 weeks old male Balb/Cj strain mice were randomly divided into the quiet ordinary diet group (CC group), the aerobic exercise normal diet group (group EC), the high fat and high sugar diet quiet group (HC group), the high fat and high sugar diet. The oxygen exercise group (group HE), each group of 6.EC groups and HE groups of mice in group HE for 6 weeks of treadmill experiment, the speed increased from 10m/min to 15m/min, each week increased one time (1m/min), 5 times a week, each time was 45min, the slope was 0.CC and EC group feed, the HC and HE groups were fed high sugar and high fat diet. Experimental animals. Before death, the level of fasting blood glucose (FBG) was detected by the Roche glucose meter. After being killed, the content of fasting insulin (FIN) was detected by ELISA method and the insulin sensitivity index (ISI) and insulin resistance index (IR) were calculated. The NOS activity of the gastrocnemius, NO content and the expression of SIRT1 protein in the gastrocnemius muscle of mice were measured by the biochemical kit. The data were analyzed with SPSS20.0 software. The results were as follows: (1) the level of FBG and FIN in group HC were higher than that in group CC, group HE, group EC and CC group, and the effect of aerobic exercise and high glucose diet on FBG level of mice were all significantly (P0.01). 0.05): ISI of group HC mice was lower than group CC, HE group and EC group were higher than group HC, group CC, aerobic exercise and high sugar and high fat diet intervention were significant (P0.01), IR in HC group was higher than that of CC group, and the effect of aerobic exercise and high sugar diet intervention on mice was significant. (2) aerobic exercise. The effects of dynamic and dietary intervention on the activity of gastrocnemius tNOS and the interaction of iNOS activity were all significantly (P0.01); the tNOS activity of the gastrocnemius muscle in the group HE mice was lower than that of the HC group (P0.01), the tNOS activity of the EC group was higher than that of the CC group, and the activity of the EC group was not different from that of the CC group; the effects of aerobic exercise and high sugar and high fat diet on the activity of the calastus muscle in mice were all affected. Significantly (P0.01), the activity of cNOS in the gastrocnemius muscle of group HE was higher than that in group HC, and in group EC was higher than that in group CC. (3) the interaction of aerobic exercise and high sugar diet intervention on the NO content of the gastrocnemius muscle of mice was significantly affected (P0.05); after 6 weeks, the HE group was lower than the HC group, and the effect of aerobic exercise on the NO content of the gastrocnemius muscle in high glucose and high fat diet mice was significantly (P0.05). (4) the interaction of aerobic exercise and high fat diet intervention on the expression of SIRT1 protein in the gastrocnemius muscle of mice was significantly affected (P0.05). The expression of SIRT1 protein in the gastrocnemius muscle of group HE was higher than that of the HC group. The effect of aerobic exercise on the expression of gastrocnemius SIRT1 protein in high glucose and high fat diet mice was significant (P0.01), and no effect on the normal diet mice. Conclusions: (1) aerobic exercise reduces the fasting blood glucose level and fasting insulin content in high glucose and high fat diet mice, improves insulin sensitivity and reduces insulin resistance in high glucose and high fat diet mice. (2) aerobic exercise reduces the tNOS activity of the gastrocnemius in high glucose and high fat diet mice, and helps to alleviate the NO content of high glucose and high fat diet mice. (3) aerobic exercise significantly increased the expression of SIRT1 protein in gastrocnemius muscle of mice fed with high glucose and high-fat diet.
【學(xué)位授予單位】：河北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R587.1

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