本文選題：花生 + 熱加工��； 參考：《食品科學(xué)》2017年11期

【摘要】：花生過(guò)敏能引起蕁麻疹、嘔吐甚至休克等癥狀,通常是終生性過(guò)敏。本研究運(yùn)用加熱處理、半胱氨酸還原對(duì)Ara h2進(jìn)行單獨(dú)和復(fù)合處理,采用圓二色譜、紫外掃描光譜、間接競(jìng)爭(zhēng)酶聯(lián)免疫吸附法分別檢測(cè)蛋白加工前后的結(jié)構(gòu)與抗原性變化。結(jié)果顯示,單一的加熱和半胱氨酸還原處理過(guò)的蛋白結(jié)構(gòu)與抗原性只發(fā)生部分變化。而復(fù)合處理中,在加熱條件下,半胱氨酸還原蛋白的能力明顯增強(qiáng),還原后的蛋白二級(jí)結(jié)構(gòu)與三級(jí)結(jié)構(gòu)發(fā)生大幅變化,抗原性顯著降低。研究結(jié)果表明,二硫鍵對(duì)于蛋白Ara h2結(jié)構(gòu)穩(wěn)定具有重要作用,二硫鍵被還原和加熱過(guò)程破壞后,蛋白的抗原性和結(jié)構(gòu)即發(fā)生重大變化。還原協(xié)同加熱處理有望大幅改善2S球蛋白的結(jié)構(gòu)和致敏性,可以作為蛋白脫敏加工的備選方法。
[Abstract]:Peanut allergies can cause symptoms such as urticaria, vomiting and even shock, usually lifelong allergies. In this study, Ara H2 was treated by heating and cysteine reduction. Circular dichroism (CD), UV scanning spectroscopy and indirect competitive enzyme linked immunosorbent assay (Elisa) were used to detect the structure and antigenicity of Ara H2 before and after processing. The results showed that the structure and antigenicity of the proteins treated with single heating and cysteine reduction only partially changed. In the compound treatment, the ability of cysteine reducing protein was significantly enhanced, the secondary and tertiary structure of the reduced protein changed significantly, and the antigenicity decreased significantly. The results show that disulfide bond plays an important role in the structural stability of protein Ara H2. After the disulfide bond is reduced and heated, the antigenicity and structure of the protein changes greatly. The structure and sensitivities of 2S globulin can be greatly improved by reducing and co-heating, and it can be used as an alternative method for protein desensitization.
【作者單位】： 南昌大學(xué)食品科學(xué)與技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室;南昌大學(xué)資源環(huán)境與化工學(xué)院;南昌大學(xué)食品學(xué)院;江西省兒童醫(yī)院感染科;江西省婦幼保健院臨床實(shí)驗(yàn)室;南昌大學(xué)中德聯(lián)合研究院;
【基金】：國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)項(xiàng)目(2013AA102205) 國(guó)家自然科學(xué)基金地區(qū)科學(xué)基金項(xiàng)目(31260411;81260103;31660446) 江西省青年科學(xué)家培養(yǎng)對(duì)象計(jì)劃項(xiàng)目(20142BCB23007) 江西省自然科學(xué)基金項(xiàng)目(20114BAB205012)
【分類號(hào)】：R593.1;TS201.2

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