本文選題：高脂血癥 + 大白鼠��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本實(shí)驗(yàn)的開展,主要有以下幾個(gè)目的：第一,研究動(dòng)物機(jī)體在高脂血癥條件下動(dòng)物的機(jī)體血脂水平的變化；第二,通過高脂血癥動(dòng)物模型,研究動(dòng)物小腸組織結(jié)構(gòu)變化,以及使用乳酸菌的預(yù)防、治療,探究其組織結(jié)構(gòu)有何規(guī)律性的改變；第三,高脂血癥條件下,對比乳酸菌治療與藥物以及飲食調(diào)節(jié)實(shí)驗(yàn)動(dòng)物腸管組織結(jié)構(gòu)變化,為合理治療高脂血癥提供一定理論依據(jù)。本次實(shí)驗(yàn)的實(shí)驗(yàn)動(dòng)物為3-4月齡SD大鼠,雌雄參半,通過飼喂高脂飼料建立SD大鼠高脂血癥模型,檢測建模成功后將其隨機(jī)分為10組,分別是：空白組、高脂組、普通飼料組、藥物組、預(yù)防1組、預(yù)防2組、預(yù)防3組、治療1組、治療2組、治療3組。按照相應(yīng)的實(shí)驗(yàn)方法處理,采樣進(jìn)行腸管各段的組織學(xué)切片,拍照測量相應(yīng)數(shù)據(jù)。采用Graph Pad Prism 5 Tow way-ANOVA Ordinary軟件進(jìn)行數(shù)據(jù)統(tǒng)計(jì)分析。數(shù)據(jù)采用均值(x)±標(biāo)準(zhǔn)差(SD)表示。結(jié)果以P0.05表示統(tǒng)計(jì)學(xué)意義結(jié)果,組間差異用LSD檢驗(yàn)。實(shí)驗(yàn)結(jié)果顯示：1.本實(shí)驗(yàn)成功建立了SD大鼠的高脂血癥模型；2.建模后不同時(shí)間段SD大鼠小腸組織學(xué)變化如下：30d和60d的十二指腸、空腸、回腸腸絨毛高度高于90d,隱窩深度淺于90d,黏膜層厚度、內(nèi)環(huán)肌和外縱肌層厚度厚于90d；30d和60d的腸絨毛高度、隱窩深度、黏膜層厚度、內(nèi)環(huán)肌和外縱肌層厚度相差不大；3.建模后相同時(shí)期不同實(shí)驗(yàn)組SD大鼠小腸組織學(xué)變化如下：十二指腸、空腸、回腸的絨毛高度空白組最高,其次依次是藥物組、預(yù)防1組、普通飼料組,其余預(yù)防組和治療組和高脂組相差不大。十二指腸、空腸、回腸的隱窩深度空白組最淺,其次依次是藥物組、預(yù)防1組、普通飼料組,其余預(yù)防組和治療組和高脂組相差不大。十二指腸、空腸、回腸的黏膜層厚度、內(nèi)環(huán)肌和外縱肌層厚度中空白組最厚,其次依次是藥物組、預(yù)防1組、普通飼料組,其余預(yù)防組和治療組和高脂組相差不大。
[Abstract]:The main objectives of this experiment are as follows: first, to study the changes of blood lipid levels in animals with hyperlipidemia; second, to study the changes of tissue structure of small intestine by hyperlipidemia animal model. And the prevention and treatment of lactic acid bacteria, to explore the regularity of its tissue structure changes; third, under hyperlipidemia conditions, compared with the treatment of lactic acid bacteria and drugs, as well as diet regulation of experimental animals intestinal tissue structure changes, To provide a theoretical basis for the rational treatment of hyperlipidemia. The experimental animals in this experiment were SD rats of 3-4 months old, male and female were mixed. SD rats were fed with high fat diet to establish hyperlipidemia model, and were randomly divided into 10 groups: blank group, hyperlipidemia group, common diet group, and normal diet group, after successful detection and modeling, the SD rats were randomly divided into 10 groups, which were: blank group, hyperlipidemia group, and normal diet group. Drug group, prevention group, treatment group and treatment group. According to the corresponding experimental method, the histological sections of each segment of the intestinal tube were sampled and the corresponding data were taken and measured. The data were analyzed by Graph Pad Prism 5 Tow way-ANOVA Ordinary software. The data are expressed by mean value (x) 鹵standard deviation (SDD). Results the results were statistically significant with P0.05, and the differences between groups were tested by LSD. The result of the experiment shows that 1: 1. The hyperlipidemia model of SD rats was established successfully. The histological changes of small intestine in SD rats at different time points were as follows: duodenum, jejunum, ileum villi were higher than 90 days, crypt depth was lower than 90 days, mucosal layer thickness, thickness of inner ring muscle and outer longitudinal muscle layer were thicker than 90 d and 60 d, intestinal villi height of duodenum, jejunum and ileum were more than 90 days. The depth of recess, the thickness of mucosal layer, the thickness of inner ring muscle and the outer longitudinal muscle layer were similar. The changes of intestinal histology of SD rats in the same period after modeling were as follows: duodenum, jejunum, ileum, villi height blank group was the highest, followed by drug group, prevention group 1, common feed group, and the following were the following. There was no significant difference between the other prevention and treatment groups and the high fat group. The depth blank group of duodenum jejunum and ileum was the shallowest, followed by drug group, prevention group 1, general feed group, other prevention group and treatment group and high fat group. The mucosal thickness of duodenum, jejunum and ileum, the thickness of inner ring muscle and external longitudinal muscle layer were the thickest in blank group, followed by drug group, prevention group 1, general diet group, other prevention group and treatment group and high fat group.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R589.2

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本文編號(hào)：1955083