本文選題：硼酸化合物 + 葡萄糖傳感器��； 參考：《大連理工大學(xué)》2015年碩士論文

【摘要】：近年來,全球范圍內(nèi)糖尿病患者數(shù)量的不斷增加引起了人們對(duì)糖尿病的高度重視,各個(gè)領(lǐng)域的科研工作者們相繼對(duì)葡萄糖傳感器產(chǎn)生了濃厚的興趣。目前,應(yīng)用最廣泛的血糖儀是一種電流型葡萄糖傳感器。由于這種葡萄糖傳感器易受葡萄糖氧化酶活性的影響,因此發(fā)展無(wú)酶型葡萄糖傳感器是當(dāng)前研究的熱點(diǎn)。本文利用了硼酸化合物具有識(shí)別糖的特性并借助DFT理論計(jì)算,設(shè)計(jì)合成了一系列的雙硼酸化合物。將穩(wěn)定的硼酸化合物代替易失活的酶作為葡萄糖的識(shí)別分子,并結(jié)合其在四十二烷基摻雜的聚合物膜離子選擇性電極上能產(chǎn)生陰離子電位響應(yīng)的特點(diǎn),建立了基于硼酸化合物識(shí)別的電位型葡萄糖傳感器,并對(duì)其選擇性、靈敏度等方面進(jìn)行了應(yīng)用研究。具體研究?jī)?nèi)容如下：1、發(fā)展了基于硼酸識(shí)別的液膜電極電位型葡萄糖傳感器的新方法。依據(jù)硼酸能與1,2-或1,3-二羥基化合物結(jié)合生成硼酸酯的性質(zhì)并利用DFT理論計(jì)算,設(shè)計(jì)合成了DBA、DBA1和DBA2三種雙硼酸化合物。通過對(duì)三者和其與葡萄糖結(jié)合生成的硼酸酯的光物理性質(zhì)及其電化學(xué)性質(zhì)的探究和對(duì)識(shí)別分子的選擇、識(shí)別分子的濃度以及緩沖溶液pH的優(yōu)化,選擇了10-5 M DBA作為識(shí)別分子在pH 8.0的磷酸緩沖溶液中作為檢測(cè)葡萄糖的最優(yōu)條件。利用DBA作為識(shí)別分子的聚合物液膜電極對(duì)葡萄糖檢出限為0.2 mM,線性范圍為1-100 mM,實(shí)現(xiàn)了對(duì)葡萄糖的高選擇性檢測(cè)且不受干擾物質(zhì)的影響。將此電位型傳感器應(yīng)用于實(shí)際血樣的檢測(cè),所得結(jié)果與商品化的血糖儀相比數(shù)據(jù)吻合。此研究方法的建立為臨床醫(yī)學(xué)中血糖的測(cè)定提供了新的思路。2、發(fā)展了基于硼酸識(shí)別的固體接觸式電極電位型葡萄糖傳感器。本研究建立了具有離子-電子傳導(dǎo)特性的導(dǎo)電聚合物聚辛基噻吩(POT)和C60作為傳導(dǎo)層的固體接觸式電極和無(wú)傳導(dǎo)層的固體接觸式電極。以DBA作為識(shí)別分子,摻雜TDDA+Cl-的聚合物液膜作為電極的敏感膜,通過傳導(dǎo)層的離子-電子傳導(dǎo)作用,將溶液中葡萄糖濃度的變化間接轉(zhuǎn)換成電信號(hào),據(jù)此實(shí)現(xiàn)了對(duì)葡萄糖的檢測(cè)。本研究突破了傳統(tǒng)離子選擇性電極需要內(nèi)充液的缺點(diǎn),通過對(duì)傳導(dǎo)層材料的優(yōu)化,建立了一種新型的固體接觸式電極電位型葡萄糖傳感器,為發(fā)展便攜式、微型化的葡萄糖傳感器提供了新方法。
[Abstract]:In recent years, the increasing number of diabetes patients worldwide has aroused great attention to diabetes, and researchers in various fields have been interested in glucose sensors. At present, the most widely used blood glucose sensor is an amperometric glucose sensor. Because the glucose sensor is easily affected by the activity of glucose oxidase, the development of non-enzymatic glucose sensor is a hot topic. In this paper, a series of diboric acid compounds were designed and synthesized by using the properties of boric acid compounds which recognize sugar and calculated by DFT theory. The stable boric acid compound was used as the recognition molecule of glucose instead of the inactivated enzyme, and the anion potential response could be produced on the ion selective electrode of 42 alkyl doped polymer membrane. A potentiometric glucose sensor based on boric acid recognition was established, and its selectivity and sensitivity were studied. The main contents are as follows: 1. A new method of liquid membrane electrode potentiometric glucose sensor based on boric acid recognition is developed. On the basis of the properties of boric acid binding with 1t2- or 1ta-3-dihydroxyl compounds and the calculation of DFT theory, three kinds of diboric acid compounds, DBA- DBA1 and DBA2, were designed and synthesized. By exploring the photophysical properties and electrochemical properties of these three borates and borate bound to glucose, the selection of recognition molecules, the concentration of recognition molecules and the pH of buffer solution were optimized. 10-5 M DBA was selected as the recognition molecule in pH 8.0 phosphoric acid buffer solution as the optimal condition for glucose detection. The detection limit of glucose is 0.2 mm and the linear range is 1-100 mm by using the polymer liquid membrane electrode with DBA as the recognition molecule. The detection of glucose is highly selective and unaffected by interfering substances. The potential sensor is applied to the detection of actual blood samples, and the results are in agreement with the commercial glycometer data. This method provides a new idea for the determination of blood glucose in clinical medicine and develops a solid contact electrode potentiometric glucose sensor based on boric acid recognition. In this study, solid contact electrodes with conducting polymer polyoctylthiophene (POT) and C60 as conducting layers and solid contact electrodes without conducting layer were established. Using DBA as the recognition molecule and TDDA Cl- doped polymer liquid film as the sensitive membrane of the electrode, the change of glucose concentration in the solution is indirectly converted into an electrical signal through the ion-electron conduction in the conduction layer. On the basis of this, the detection of glucose was realized. In this study, the shortcoming of the traditional ion selective electrode needed to be filled with liquid was overcome. By optimizing the conductive layer material, a new type of solid contact electrode potentiometric glucose sensor was established to develop portable glucose sensor. The miniaturized glucose sensor provides a new method.
【學(xué)位授予單位】：大連理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R587.1;TP212

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 彭秀英;糖類與有機(jī)小分子相互作用的電化學(xué)研究及應(yīng)用[D];西南大學(xué);2007年

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本文編號(hào)：1923602