本文選題：尿酸 + 學(xué)習(xí)記憶能力�。� 參考：《山東醫(yī)藥》2017年22期

【摘要】：目的觀察不同水平血尿酸對(duì)大鼠學(xué)習(xí)記憶能力的影響。方法將48只大鼠隨機(jī)分為對(duì)照組、輕度組、中度組、重度組各12只,后三組采用腹腔注射氧嗪酸鉀鹽的方法制備不同水平的高尿酸血癥(HUA)動(dòng)物模型,對(duì)照組大鼠腹腔注射等量生理鹽水,均連續(xù)注射給藥28 d。采用Morris水迷宮試驗(yàn)觀察各組大鼠的學(xué)習(xí)記憶能力,化學(xué)發(fā)光法檢測(cè)大鼠血清超氧化物歧化酶(SOD),免疫組織化學(xué)法、酶聯(lián)免疫吸附試驗(yàn)觀察大鼠海馬區(qū)β-淀粉樣蛋白1-42(Aβ1-42)表達(dá)情況。結(jié)果對(duì)照組、輕度組、中度組、重度組大鼠潛伏期分別為(19.6±5.7)、(39.1±6.5)、(36.2±5.6)、(33.7±8.2)s,穿越平臺(tái)次數(shù)分別為(3.2±0.8)、(2.0±0.6)、(2.1±0.4)、(2.3±0.5)次,輕度組、中度組、重度組與對(duì)照組相比,P均0.01。對(duì)照組、輕度組、中度組、重度組大鼠血清SOD活性分別為(403.9±16.3)、(356.9±22.0)、(372.9±15.7)、(382.1±16.2)U/m L,海馬組織Aβ1-42表達(dá)陽(yáng)性細(xì)胞數(shù)分別為(8.5±2.3)、(70.6±20.5)、(47.5±11.2)、(30.7±7.9)個(gè),海馬組織Aβ1-42相對(duì)表達(dá)量分別為(11.1±3.1)、(46.9±6.3)、(40.8±5.3)、(35.6±5.6)pg/mg,兩兩相比,P均0.05。結(jié)論高于正常的不同水平血尿酸均可導(dǎo)致大鼠學(xué)習(xí)記憶能力下降,但隨著血尿酸水平的升高大鼠學(xué)習(xí)記憶能力下降程度減輕,其機(jī)制可能與尿酸的抗氧化及促氧化雙重特性有關(guān)。
[Abstract]:Objective to observe the effects of different levels of serum uric acid on learning and memory ability in rats. Methods Forty-eight rats were randomly divided into control group, mild group, moderate group and severe group, 12 rats in each group. Rats in control group were intraperitoneally injected with the same amount of normal saline for 28 days. The learning and memory abilities of rats in each group were observed by Morris water maze test. The expression of 尾 -amyloid protein 1-42A 尾 1-42 in hippocampus of rats was detected by chemiluminescence assay, immunohistochemical method and enzyme-linked immunosorbent assay. Results the latencies of the control group, mild group, moderate group and severe group were 19.6 鹵5.7 and 39.1 鹵6.5, respectively, and the times of crossing the platform were 3.2 鹵0.8, 2.1 鹵0.4 and 2.3 鹵0.5, respectively. Compared with the control group, the latency of the mild group, the moderate group and the severe group were all 0.011.Results there was no significant difference between the mild group, the moderate group and the control group (P > 0.05). In the control group, mild group, moderate group and severe group, the serum SOD activity was 403.9 鹵16.3fen, 356.9 鹵22.0mg / L, respectively, and the number of A 尾 1-42 positive cells in hippocampus was 70.6 鹵20.57.5 鹵11.20.70 鹵7.9respectively, and the relative expression of A 尾 1-42 in hippocampus was 11.1 鹵3.446.30.30.8 鹵6.30.8 鹵5.336.36.6pgmg / g, respectively, compared with 0.055.57.9in hippocampus, the relative expression of A 尾 1-42 in hippocampus was 11.1 鹵3.1 鹵6.30.38. 6 鹵5.6pgmg / g / g, respectively. Conclusion different levels of serum uric acid above normal can decrease the ability of learning and memory in rats, but with the increase of serum uric acid level, the ability of learning and memory decreases. The mechanism may be related to the antioxidant and pro-oxidation characteristics of uric acid.
【作者單位】： 南昌大學(xué)瑪麗女王學(xué)院;南昌大學(xué)第一臨床醫(yī)學(xué)院;南昌大學(xué)第二附屬醫(yī)院;
【分類號(hào)】：R589.7

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