本文選題：富硒益生菌 + 肥胖�。� 參考：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：在過(guò)去的幾十年里,肥胖及其并發(fā)癥的發(fā)生率在全球范圍內(nèi)大大增加了。其原因除了食物攝入的增多和體力活動(dòng)的減少,越來(lái)越多的證據(jù)表明胃腸道中的微生物(即腸道菌群)與肥胖癥的發(fā)生和相關(guān)代謝息息相關(guān)。微量元素硒是人和動(dòng)物重要的的必須營(yíng)養(yǎng)素。由于許多國(guó)家的飲食中硒往往低于最佳含量,因此通過(guò)在食物中補(bǔ)充或強(qiáng)化硒的添加已經(jīng)越來(lái)越普遍。市售硒補(bǔ)充劑包括無(wú)機(jī)礦物鹽(例如亞硒酸鈉和硒酸鹽)和有機(jī)硒(如富硒酵母)。研究目的這項(xiàng)研究的目的是評(píng)估富硒益生菌(SP)對(duì)高脂肪飲食(HFD)老鼠的脂類(lèi)代謝,抗氧化狀態(tài),組織病理學(xué)損傷和生化指標(biāo)的影響。試驗(yàn)設(shè)計(jì)80只4周齡,平均體重23克的雌性白化ICR老鼠被隨機(jī)分成5個(gè)組(n=16)。A組老鼠飼喂正常飼料作為陰性對(duì)照組,B組老鼠飼喂HFD(15%)作為陽(yáng)性對(duì)照組,C組老鼠飼喂HFD的同時(shí)給予益生菌(P,包含嗜酸乳桿菌1011/mL CFU和釀酒酵母菌109/mLCFU),D組和E組老鼠分別飼喂HFD的同時(shí)給予亞硒酸鈉(SS)/SP(折算的硒含量都為0.3μg/g)�；A(chǔ)飲食的硒含量以國(guó)家研究委員會(huì)(NRC,1995)制定的0.05 mg/kg為標(biāo)準(zhǔn)。根據(jù)動(dòng)物實(shí)驗(yàn)室和南京農(nóng)業(yè)大學(xué)動(dòng)物福利委員會(huì)(經(jīng)江蘇省科學(xué)技術(shù)廳批準(zhǔn))的要求飼養(yǎng)動(dòng)物并開(kāi)展動(dòng)物試驗(yàn)。老鼠被安置在室溫25±2℃的室內(nèi),并提供12小時(shí)黑暗/12小時(shí)光照。用于本研究的SP和P中包含兩個(gè)益生菌菌株,嗜酸乳桿菌1011/mL(CFU)和釀酒酵母菌109/mL(CFU)。使用AF-610原子熒光光譜儀進(jìn)行檢測(cè),SP中的硒含量為10.0 mg/L,且其中大于90%是有機(jī)硒,大于75%是硒代蛋氨酸。C組和E組中益生菌的量是相同的,嗜酸乳桿菌為0.25*1011/mL(CFU),釀酒酵母菌為0.25*109/mL(CFU)。SP和SS與高脂肪日糧混勻后碾成粉末。檢測(cè)總蛋白(TP)、總膽固醇(TC)、丙氨酸轉(zhuǎn)氨酶(AST)、天門(mén)冬氨酸轉(zhuǎn)氨酶(ALT)的水平。LDL,HDL和TG試劑盒由南京建成生物工程研究所提供。測(cè)定GSH-Px,SOD,CAT活性和MDA水平。為了確定SP影響脂質(zhì)內(nèi)穩(wěn)態(tài)機(jī)制,我們分析了與脂質(zhì)代謝相關(guān)基因的mRNA水平(CPT1,CPT2,ACOX2,ACAT2,FAS,LPL,PPARα,PPARγ and SREBP2)。引物序列是通過(guò)Primer 5.0在線軟件設(shè)計(jì)的,序列見(jiàn)表1。在試驗(yàn)的結(jié)束時(shí)(第5周)采集老鼠血液和肝臟樣品,用于檢測(cè)脂質(zhì)代謝、抗氧化狀態(tài)、病理學(xué)變化和相關(guān)基因的表達(dá)。試驗(yàn)結(jié)果B組老鼠的體重比其他幾個(gè)組(A,C,D和E)大。肝組織的病理學(xué)檢查表明,與B組相比C,D,E組的肝組織損傷更小,肝細(xì)胞內(nèi)的脂肪空泡更少。C,D,E組的血清AST,ALT,TC,TG和LDL-C比B組顯著下降,TP和HDL-C比B組顯著上升。C,D,E組的SOD,CAT和GSH-Px酶活性比B組顯著提高,MDA水平則比B.組顯著下降。與B組相比,C,D,E組緩解了肝細(xì)胞內(nèi)脂質(zhì)的堆積。大體而言,添加P,SS或 SP 上調(diào)了 CPT1,CPT2,ACAT2 ACOX2 和 PPARα 的 mRNA 表達(dá)水平,下調(diào)了FAS,LPL,PPARy和SREBP1的mRNA表達(dá)水平,這些基因都參與了脂質(zhì)的代謝。在這幾組中,增加SP對(duì)改善高脂飲食老鼠的脂質(zhì)代謝,抗氧化狀態(tài),病理學(xué)變化和相關(guān)基因表達(dá)的效果最理想。結(jié)論高脂飲食可以顯著促進(jìn)肥胖、氧化應(yīng)激的發(fā)生,造成脂質(zhì)重分布。而益生菌、富硒益生菌和亞硒酸鈉可以顯著改善高脂飲食誘導(dǎo)肥胖老鼠的脂質(zhì)分布、氧化狀態(tài)和相關(guān)基因的表達(dá)。
[Abstract]:In the past few decades, the incidence of obesity and its complications has increased worldwide. In addition to the increase in food intake and decrease in physical activity, more and more evidence suggests that microorganisms in the gastrointestinal tract (intestinal flora) are associated with obesity and related metabolism. Trace elements selenium is human and active. Important nutrients are essential. As selenium is often lower in the diet of many countries, it is becoming more and more common to add or strengthen selenium in food. The market selenium supplements include inorganic mineral salts (such as sodium selenite and selenate) and organic selenium (such as selenium enriched yeast). The purpose of this study is to study the purpose of this study The effects of selenium enriched probiotics (SP) on lipid metabolism, antioxidant status, histopathological damage and biochemical indexes in high fat diet (HFD) mice were evaluated. 80 4 weeks old female albino ICR mice with an average weight of 23 grams were randomly divided into 5 groups (n=16) group.A rats fed normal diet as negative control group, and B group mice fed HFD (15). As a positive control group, group C rats were fed HFD with probiotics (P, including Lactobacillus acidophilus 1011/mL CFU and Saccharomyces cerevisiae 109/mLCFU), D group and E group were fed HFD (SS) /SP (SS) /SP (0.3 mu g/g). The selenium content of basic diet was formulated by National Research Committee (1995) 0.05 mg/kg was the standard. Animals were raised in accordance with animal laboratories and the animal welfare committee of Nanjing Agricultural University (approved by the Jiangsu Department of science and Technology). Animals were fed and conducted animal tests. Mice were placed in room at room temperature of 25 + 2 and provided 12 hours of dark /12. Two probiotic strains were included in the study's SP and P. Lactobacillus acidophilus 1011/mL (CFU) and Saccharomyces cerevisiae 109/mL (CFU) were detected by AF-610 atomic fluorescence spectrometer. The selenium content in SP was 10 mg/L, and more than 90% was organic selenium, and greater than 75% was the same amount of probiotics in the selenium methionine.C group and the E group, and the Lactobacillus acidophilus was 0.25*1011/mL (CFU) and the Saccharomyces cerevisiae was 0.25*109/mL. (CFU).SP and SS were mixed with high fat diet to powder. The total protein (TP), total cholesterol (TC), alanine aminotransferase (AST), the level.LDL of aspartate aminotransferase (ALT), HDL and TG kits were provided by the Institute of bioengineering in Nanjing. We analyzed the mRNA levels of lipid metabolism related genes (CPT1, CPT2, ACOX2, ACAT2, FAS, LPL, PPAR a, PPAR gamma and SREBP2). The primer sequence was designed through the Primer 5 online software. The sequence was shown in table 1. to collect blood and liver samples at the end of the test (fifth weeks) for the detection of lipid metabolism, antioxidant status, and pathological changes. The body weight of the B group was larger than that of the other groups (A, C, D and E). The pathological examination of the liver tissue showed that the liver tissues of the group of C, D, and E were smaller than the B group, and the lipid vacuoles in the liver cells were less.C, D. The activity of SOD, CAT and GSH-Px was significantly higher than that in the B group, while the MDA level was significantly lower than that in the B. group. The levels of C, D, E groups relieved the accumulation of lipid in the liver cells. In these groups, increasing SP has the best effect on improving lipid metabolism, antioxidant status, pathological changes and related gene expression in high fat diet mice. Conclusion high fat diet can significantly promote obesity, oxidative stress and lipid redistribution, while probiotics, selenium enriched probiotics and sodium selenite can be used. To significantly improve lipid distribution, oxidative status and related gene expression in obese rats induced by high-fat diet.

【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S816;R589.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Stephan Ebner;Linda N Smug;Wolfgang Kneifel;Seppo J Salminen;Mary Ellen Sanders;;Probiotics in dietary guidelines and clinical recommendations outside the European Union[J];World Journal of Gastroenterology;2014年43期

2 ;Protective effect of selenium-enriched lactobacilluson CCI_4-induced liver injury in mice and its possible mechanisms[J];World Journal of Gastroenterology;2005年37期

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本文編號(hào)：1846824