本文選題：PRF + 富血小板纖維蛋白��； 參考：《安徽醫(yī)科大學學報》2017年07期

【摘要】：目的觀察富血小板纖維蛋白(PRF)對糖尿病動物模型皮膚創(chuàng)面的治療作用及療效。方法將20只大白兔隨機分為空白對照組(4只)、糖尿病模型組(4只)、重組人表皮生長因子凝膠(rhEGF)組(4只),PRF組(8只),將糖尿病模型組、rhEGF組、PRF組建立糖尿病動物創(chuàng)面模型,4組動物創(chuàng)面分別予以1%碘伏清創(chuàng)、1%碘伏清創(chuàng)、rhEGF外敷、PRF外敷。觀察各組創(chuàng)面面積及容積變化情況,標本予以HE染色觀察創(chuàng)面組織生長情況,免疫組化標記血管內(nèi)皮生長因子(VEGF)蛋白,計算VEGF平均光密度。結果干預后3、7 d顯示,rhEGF組、PRF組創(chuàng)面面積和容積縮小的速度高于空白對照組及糖尿病模型組(P0.05)。第2、3周末大體病理觀察rhEGF組、PRF組創(chuàng)面組織表皮覆蓋完全,真皮結構致密,皮膚結構基本恢復正常,有大量毛細血管存在。第2、3周末標本VEGF平均光密度蛋白含量,rhEGF組、PRF組顯著高于空白對照組、糖尿病模型組(P0.05)。結論 PRF可顯著促進糖尿病兔皮膚創(chuàng)面的愈合。
[Abstract]:Objective to observe the therapeutic effect and efficacy of platelet rich fibrin (PRF) on skin wound of diabetic animal model. Methods Twenty rabbits were randomly divided into control group (n = 4), diabetic model group (n = 4), recombinant human epidermal growth factor gel (rhEGF) group (n = 4) and PRF group (n = 8). The wounds of the 4 groups were treated with 1% Iodophor debridement and 1% Iodophor debridement with rhEGF and PRF respectively. The changes of wound area and volume in each group were observed. The growth of wound tissue was observed by HE staining. Vascular endothelial growth factor (VEGF) protein was labeled with immunohistochemistry and the mean optical density of VEGF was calculated. Results 3 days after intervention, the rate of wound area and volume reduction in rhEGF group was higher than that in blank control group and diabetic model group (P 0.05). At the end of the 2nd week, the histopathological observation showed that in rhEGF group, the wound tissue was completely covered by epidermis, the dermis structure was compact, the skin structure basically returned to normal, and a large number of capillaries existed. At the end of the 2nd week, the average optical density protein content of VEGF in the rhEGF group was significantly higher than that in the blank control group, and that in the diabetic model group was significantly higher than that in the control group (P 0.05). Conclusion PRF can significantly promote the healing of diabetic rabbit skin wounds.
【作者單位】： 安徽醫(yī)科大學第一附屬醫(yī)院整形外科;
【基金】：國家自然科學基金(編號:81171829)
【分類號】：R587.2

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