本文選題：高低濃度波動的葡萄糖 + 心肌細(xì)胞　； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究阿托伐他汀鈣(Atorvastatin,atv)聯(lián)合替米沙坦(Telmisartan,TEL)對波動性高糖誘導(dǎo)心肌細(xì)胞凋亡的影響。方法:心肌細(xì)胞在低濃度葡萄糖(正常濃度)環(huán)境下貼壁成長,取對數(shù)期生長的心肌細(xì)胞分為空白對照組A組,波動性高糖組B組,波動性高糖環(huán)境下不同藥物干預(yù)(分別為高濃度阿托伐他汀鈣10.0umol/l、高濃度替米沙坦1×10-6mol/l、高濃度的二者聯(lián)合使用)的C1-C3組(阿托伐他汀鈣及替米沙坦?jié)舛染鶕?jù)查找相關(guān)參考文獻(xiàn)所定的高濃度)。建立起波動性高糖的損傷模型,以及藥物干預(yù)后繼續(xù)培養(yǎng)24h后通過細(xì)胞增殖及細(xì)胞毒性檢測試劑盒(CCK-8)檢測心肌細(xì)胞的增殖,細(xì)胞凋亡檢測試劑盒(TUNEL)檢測心肌細(xì)胞的凋亡。結(jié)果:(1)與對照組比較,波動性高糖組及各藥物干預(yù)組的心肌細(xì)胞增殖均減低。(2)與單純的波動性高糖組比較,阿托伐他汀鈣、替米沙坦及二者聯(lián)合的干預(yù)組對心肌細(xì)胞的增殖均有保護(hù)作用。(3)二者聯(lián)合的藥物干預(yù)組與單一的藥物干預(yù)組比較,二者聯(lián)合使用組對心肌細(xì)胞的凋亡的保護(hù)作用更加明顯,要優(yōu)于單一使用任一種藥物。討論:波動性高糖的環(huán)境對心肌細(xì)胞具有明顯的損傷作用,阿托伐他汀鈣、替米沙坦及二者聯(lián)合后均能降低波動性高糖對心肌細(xì)胞的損傷作用,且二者聯(lián)合對心肌細(xì)胞的保護(hù)作用更加顯著,明顯優(yōu)于單一使用任一種藥物。
[Abstract]:Aim: to study the effects of Atto vastvastatin calcium and telmisartanol on apoptosis of cardiomyocytes induced by volatile hyperglycemia. Methods: cardiomyocytes grew in low concentration glucose (normal concentration). The cardiomyocytes growing in logarithmic phase were divided into control group A and volatile high glucose group B, respectively. The C1-C3 group (Atto vastatin calcium and telmisartan concentrations) in volatile high glucose environments (high concentration of Atto vastatin calcium 10.0 umoll / l, high concentration of telmisartan 1 脳 10 -6 mol / L, high concentration of both) were based on phase finding. The high concentration determined in the relevant references. The damage model of volatile high glucose was established, and the proliferation of cardiomyocytes was detected by cell proliferation and cytotoxicity test kit CCK-8, and the apoptosis of cardiomyocytes was detected by Tunel after drug intervention for 24 hours. Results compared with the control group, the proliferation of cardiomyocytes in the volatile high glucose group and the drug intervention group was decreased. (2) compared with the volatile high glucose group, Atto vastatin calcium was found in the control group. The protective effect of telmisartan and its combination on cardiomyocyte proliferation was more obvious than that of single drug intervention group, and the protective effect on cardiomyocyte apoptosis was more obvious in the combined drug intervention group than in the single drug intervention group, and the protective effect of the two groups on cardiomyocyte apoptosis was more obvious than that in the single drug intervention group. It is better to use any drug alone. Discussion: fluctuating high glucose environment has obvious damage effect on cardiomyocytes. Atto vastatin calcium, telmisartan and the combination of both can reduce the damage effect of fluctuating high glucose on cardiomyocytes. The protective effect of the two combination on cardiomyocytes was more significant than that of any single drug.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R587.2;R54

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本文編號：1790723