發(fā)布時(shí)間：2018-04-23 02:13

  本文選題：sfrp5 + 脂聯(lián)素��； 參考：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:脂聯(lián)素和分泌型卷曲相關(guān)蛋白5(secreted frizzled related protein,sfrp5)是近年來(lái)研究較熱的脂肪因子,在2型糖尿病等疾病的發(fā)生發(fā)展過(guò)程中起著重要作用。本研究通過(guò)測(cè)定正常人及2型糖尿病患者血漿脂聯(lián)素和sfrp5的水平探究其與2型糖尿病及2型糖尿病合并大血管病變的相關(guān)性。方法:1.本研究共納入2015年11月-2016年7月在川北醫(yī)學(xué)院附屬醫(yī)院內(nèi)分泌科門診及住院部就診的81例2型糖尿病患者(女38例,男43例),平齡:52.15±11.91歲。根據(jù)有無(wú)血管病變分為單純2型糖尿病組(n MVC)和2型糖尿病合并大血管病變組(MVC)。并根據(jù)大血管病變程度將MVC分為三個(gè)亞組。另于川北醫(yī)學(xué)院附屬醫(yī)院體檢中心隨機(jī)選取健康體檢者80例為正常對(duì)照組(NC組)。2.采用酶聯(lián)免疫吸附法(ELISA)測(cè)定各組空腹血漿脂聯(lián)素和sfrp5濃度,同時(shí)采集各組體重指數(shù)、空腹血糖、糖化血紅蛋白、甘油三酯、膽固醇、高密度脂蛋白、低密度脂蛋白等一般臨床指標(biāo)。3.應(yīng)用SPSS19.0統(tǒng)計(jì)軟件對(duì)所有數(shù)據(jù)進(jìn)行分析,正態(tài)分布的資料以±s表示,非正態(tài)分布資料通過(guò)對(duì)數(shù)轉(zhuǎn)換后進(jìn)行分析。多組間比較采用方差分析。相關(guān)分析采用spearman相關(guān)分析。多因素分析采用多元逐步回歸分析。以logistic回歸分析2型糖尿病合并大血管病變的主要影響因素。均以P0.05為差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:1.各組間sfrp5比較:NC與n MVC、MVC任意兩兩比較均有統(tǒng)計(jì)學(xué)意義,且呈逐漸降低趨勢(shì)(F=34.957,P=0.000);各組間脂聯(lián)素比較:NC與n MVC、MVC任意兩兩比較均有統(tǒng)計(jì)學(xué)意義,且呈逐漸降低趨勢(shì)(F=48.317,P=0.000)。2.各亞組間sfrp5比較:M1與M2、M3任意兩兩比較均有統(tǒng)計(jì)學(xué)意義,且呈逐漸降低趨勢(shì)(F=6.783,P=0.000);各亞組間脂聯(lián)素比較:M1與M2、M3任意兩兩比較均有統(tǒng)計(jì)學(xué)意義,且呈逐漸降低趨勢(shì)(F=5.067,P=0.003)。3.以sfrp5為因變量,以脂聯(lián)素、BMI、TG、TC、HDL-C、LDL-C、FBG、Hb A1c為自變量,進(jìn)行多元線性逐步回歸顯示:脂聯(lián)素、LDL-C、Hb A1c是血漿sfrp5的顯著影響因素(R2=0.621,P0.05)。4.以脂聯(lián)素為因變量,以sfrp5、BMI、TG、TC、HDL-C、LDL-C、FBG、Hb A1c為自變量,進(jìn)行多元線性逐步回歸顯示:sfrp5、LDL-C、Hb A1c是血漿脂聯(lián)素的顯著影響因素(R2=0.614,P0.05)。5.以2型糖尿病是否合并大血管病變?yōu)橐蜃兞?以sfrp5、BMI、TG、TC、HDL-C、LDL-C、FBG、Hb A1c為自變量,進(jìn)行l(wèi)ogistic回歸分析,結(jié)果表明sfrp5、BMI、TG、HDL-C進(jìn)入方程,表明他們是2型糖尿病合并大血管病變的重要影響因素。結(jié)論:1.血漿sfrp5、脂聯(lián)素均與肥胖、血糖、血脂異常、大血管病變相關(guān),或許可作為臨床上對(duì)于肥胖、2型糖尿病、血脂異常、動(dòng)脈粥樣硬化的診斷、治療及預(yù)后提供一些依據(jù),但其作用機(jī)制尚未十分清楚,需進(jìn)一步完善相關(guān)基礎(chǔ)實(shí)驗(yàn)探究其作用機(jī)制。2.sfrp5與脂聯(lián)素呈顯著正相關(guān),可能是由于二者均有脂肪細(xì)胞分泌,當(dāng)脂肪細(xì)胞功能紊亂時(shí),二者分泌均出現(xiàn)異常;同時(shí),二者在發(fā)揮生物學(xué)作用時(shí),可能為協(xié)同作用。
[Abstract]:Objective: adiponectin (adiponectin) and secretory crimp associated protein (5(secreted frizzled related protein frp5) are hot fat factors and play an important role in the development of type 2 diabetes. In this study, plasma adiponectin and sfrp5 levels were measured in normal subjects and patients with type 2 diabetes to explore their correlation with type 2 diabetes and type 2 diabetes with macroangiopathy. Method 1: 1. From November 2015 to July 2016, 81 patients with type 2 diabetes mellitus (38 female and 43 male), aged 52.15 鹵11.91 years, were enrolled in the Department of Endocrinology, Department of Endocrinology, affiliated Hospital of North Sichuan Medical College. The patients were divided into two groups: type 2 diabetes mellitus group (n MVC) and type 2 diabetes mellitus complicated with macrovascular disease group. MVC was divided into three subgroups according to the degree of macrovascular disease. In addition, 80 healthy subjects were randomly selected as normal control group (NC group) in the center of medical examination center of affiliated hospital of North Sichuan Medical College. Fasting plasma adiponectin and sfrp5 levels were measured by Elisa. Body mass index, fasting blood glucose, glycosylated hemoglobin, triglyceride, cholesterol, high density lipoprotein were collected. Low density lipoprotein and other general clinical indicators. The data of normal distribution is expressed as 鹵s, and the data of non-normal distribution is analyzed by the transformation of numbers. ANOVA was used to compare the two groups. Correlation analysis was performed by spearman correlation analysis. Multivariate stepwise regression analysis was used. The main influencing factors of type 2 diabetes with macroangiopathy were analyzed by logistic regression analysis. All with P0.05 as the difference was statistically significant. The result is 1: 1. There was a significant difference in sfrp5 comparison between each group (% NC) and n MVC (n MVC), and there was a trend of decreasing gradually (P < 0. 000), and there was a significant difference in adiponectin comparison between each group (% NC) and n MVCMVC (arbitrary pairwise comparison), and there was a decreasing trend of FN (48.317P0. 000) 路2.The ratio of adiponectin in each group was 48.317P0. 000 ~ 0. 000 and the ratio of adiponectin to n MVCMVC was significantly lower. The comparison of sfrp5 between M2M3 and M2M3 had statistical significance, and showed a decreasing trend (F = 6.783), and adiponectin was significantly higher than that of M2M3 (P < 0.01), and showed a decreasing trend of F5.067P0. 003N 路3.The ratio of adiponectin between the two subgroups was statistically significant in comparison with that of M2M3, and there was a trend of decrease in F5. 067P0. 003p0. 3 in each subgroup. The multivariate stepwise regression analysis showed that adiponectin (sfrp5) was a significant influencing factor for plasma sfrp5, and HDL-CnLDL-CU HbA1c was an independent variable. It was found that adiponectin was a significant factor affecting plasma sfrp5 (R20.621P0.05N. 4). With adiponectin as dependent variable, and sfrp5BMITGN TGG (TGN) HDL-CU LDL-CU FBGU HbA1c as independent variable, multivariate linear stepwise regression analysis showed that: sfrp5, LDL-CmHbA1c was a significant factor affecting plasma adiponectin (R20.614, P0.05. 5), and a multivariate linear stepwise regression analysis was carried out to show that: sfrp5HbA1c was a significant influence factor of plasma adiponectin. Taking type 2 diabetes mellitus with macrovascular disease as dependent variable, using sfrp5 BMITGN TGGN HDL-CnLDL-CnLDL-C FBGG HbA1c as independent variable, logistic regression analysis was carried out. The results showed that sfrp5BMITGN HDL-C entered the equation, indicating that they were important factors in type 2 diabetes mellitus with macroangiopathy. Conclusion 1. Plasma sfrp5 and adiponectin are all associated with obesity, blood glucose, dyslipidemia, macroangiopathy, which may provide some evidence for diagnosis, treatment and prognosis of obesity type 2 diabetes mellitus, dyslipidemia and atherosclerosis. However, the mechanism of adiponectin is not very clear. It is necessary to further improve the basic experiment to find out the positive correlation between the mechanism. 2. Sfrp5 and adiponectin, which may be due to both adipocytes secreting adipocytes, when the function of adipocytes is disordered. At the same time, they may be synergistic when they play a biological role.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R587.2

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相關(guān)期刊論文 前2條

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本文編號(hào)：1789985