發(fā)布時(shí)間：2018-04-19 19:52

  本文選題：系統(tǒng)性紅斑狼瘡 + Tim-　； 參考：《中國(guó)醫(yī)科大學(xué)學(xué)報(bào)》2017年07期

【摘要】：目的探討Tim-1蛋白表達(dá)及其基因多態(tài)性與系統(tǒng)性紅斑狼瘡(SLE)患者營(yíng)養(yǎng)功能指標(biāo)的相關(guān)性。方法收集126例SLE患者外周血樣;采用ELISA法檢測(cè)血清中人Tim-1蛋白水平;采用PCR-RFLP法檢測(cè)外周血DNA樣本Tim-1基因-416GC和-1454GA多態(tài)性;應(yīng)用免疫比濁法檢測(cè)血清標(biāo)本中前白蛋白、銅藍(lán)蛋白、視黃醇結(jié)合蛋白的水平;采用電化學(xué)發(fā)光法檢測(cè)血清標(biāo)本中鐵蛋白、1,25-二羥維生素D3的水平。結(jié)果血清Tim-1蛋白、前白蛋白、銅藍(lán)蛋白、視黃醇結(jié)合蛋白、鐵蛋白及1,25-二羥維生素D3的濃度分別為(249.7±30.2)pg/m L、(226±42)μg/m L、(363±95)μg/m L、(29.4±13.2)μg/m L、(355±164)ng/m L、(26.4±11.5)ng/m L;-416GC位點(diǎn)上GG基因型占11.9%,GC基因型占57.1%,CC基因型占31.0%;-1454GA位點(diǎn)上GG基因型占67.5%,GA基因型占26.2%,AA基因型占6.3%。-416GC位點(diǎn)不同基因型之間(F=0.575,P=0.564)及-1454GA位點(diǎn)不同基因型之間(F=1.255,P=0.289)Tim-1蛋白濃度均無(wú)統(tǒng)計(jì)學(xué)差異。Tim-1與前白蛋白顯著負(fù)相關(guān)(r=-0.176,P=0.033),與銅藍(lán)蛋白(r=0.205,P=0.014)和1,25-二羥維生素D3(r=0.166,P=0.042)均顯著正相關(guān)。-1454GA位點(diǎn)GG基因型患者血清中前白蛋白水平顯著降低(P=0.027),AA基因型患者血清中1,25-二羥維生素D3水平顯著降低(P=0.024)。結(jié)論血清Tim-1蛋白水平及Tim-1基因-1454GA位點(diǎn)多態(tài)性與SLE患者營(yíng)養(yǎng)功能指標(biāo)存在相關(guān)性。
[Abstract]:Objective to investigate the relationship between Tim-1 protein expression and gene polymorphism in patients with systemic lupus erythematosus (SLE). Methods Peripheral blood samples were collected from 126 patients with SLE, serum Tim-1 protein levels were detected by ELISA method, Tim-1 gene -416GC and -1454GA polymorphisms in peripheral blood DNA samples were detected by PCR-RFLP method, prealbumin and ceruloplasmin in serum samples were detected by immunoturbidimetric method. The level of retinol binding protein and the level of ferritin 1 / 25-dihydroxyvitamin D _ 3 in serum samples were determined by electrochemiluminescence (ECL). Results Serum Tim-1 protein, prealbumin, ceruloplasmin, retinol binding protein, The concentrations of ferritin and 1-dihydroxyvitamin D _ 3 were 249.7 鹵30.2)pg/m / L ~ (226 鹵42) 渭 g / mL ~ (63 鹵95) 渭 g / m ~ (-1)) 29.4 鹵13.2) 渭 g / m ~ (1 / 3) ~ (355) 鹵164)ng/m ~ (26.4) 鹵11.5)ng/m / L ~ (26.4) 鹵11.5)ng/m / L ~ (416GC), respectively. There was no significant difference in the protein concentration between the two genotypes. Tim-1 was negatively correlated with prealbumin, and was significantly negatively correlated with prealbumin, and positively correlated with r0.205P0.014) and 125-dihydroxyvitamin D3HV 0.166P0.042) in the serum of GG genotype patients with GG locus 1454GA. The level of albumin in serum of patients with AA genotype was significantly lower than that of control group (P < 0. 027), and the serum level of vitamin D 3 was significantly lower than that of control group (P < 0. 024). Conclusion the level of serum Tim-1 protein and the polymorphism of Tim-1 gene -1454GA are correlated with the nutritional function of SLE patients.
【作者單位】： 承德醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科;承德縣醫(yī)院骨外科;承德醫(yī)學(xué)院附屬醫(yī)院輸血科;
【基金】：承德市科學(xué)技術(shù)研究與發(fā)展計(jì)劃(201601A031)
【分類號(hào)】：R593.241

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 熊勤;;Tim-1蛋白在SLE患者血清中的表達(dá)及其基因多態(tài)性分析[J];數(shù)理醫(yī)藥學(xué)雜志;2016年01期

2 陳婉婷;彭傳梅;高輝;;Tim基因家族與系統(tǒng)性紅斑狼瘡關(guān)系的研究進(jìn)展[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2015年11期

3 彭傳梅;高輝;付曉野;曹向紅;董玉琳;王楊;;云南漢族人群Tim-1基因多態(tài)性與系統(tǒng)性紅斑狼瘡關(guān)聯(lián)性研究[J];中國(guó)實(shí)驗(yàn)診斷學(xué);2014年12期

4 陳杰;胡娜;蘇斌濤;崔天盆;;系統(tǒng)性紅斑狼瘡患者血清中Tim-1蛋白水平及其基因多態(tài)性[J];中國(guó)免疫學(xué)雜志;2014年01期

5 陳治中;卿吉琳;胡麗華;;Interactions of Human T Cell Immunoglobin Mucins with Apoptotic Cells[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2012年01期

6 李圣楠;黃慈波;;系統(tǒng)性紅斑狼瘡的診斷治療進(jìn)展[J];臨床藥物治療雜志;2010年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊輝;謝志軍;溫成平;;病證結(jié)合治療系統(tǒng)性紅斑狼瘡探析[J];中醫(yī)雜志;2017年13期

2 陳向華;王建吉;耿學(xué)麗;丁萌;華正祥;;Tim-1蛋白表達(dá)及其基因多態(tài)性與系統(tǒng)性紅斑狼瘡患者營(yíng)養(yǎng)功能的相關(guān)性[J];中國(guó)醫(yī)科大學(xué)學(xué)報(bào);2017年07期

3 陶鵬輝;;系統(tǒng)性紅斑狼瘡患者血清IL-17、IL-23、HMGB1表達(dá)水平及臨床意義[J];實(shí)驗(yàn)與檢驗(yàn)醫(yī)學(xué);2017年02期

4 龐盼;朱sソ，

本文編號(hào)：1774483