本文選題：糖尿病 + 巨噬細(xì)胞�。� 參考：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年12期

【摘要】：目的·通過外源性阻斷糖基化終末產(chǎn)物受體(RAGE)后,觀察RAGE效應(yīng)對糖尿病小鼠創(chuàng)面愈合過程中巨噬細(xì)胞浸潤的影響。方法·96只雄性8周齡C57BL/6J小鼠隨機(jī)分成糖尿病組(n=72)和正常對照組(n=24)。多次小劑量鏈脲佐菌素腹腔注射誘導(dǎo)糖尿病小鼠模型,并用滅菌9 mm直徑環(huán)鉆于小鼠背部制造全層皮膚缺損創(chuàng)面模型。傷后正常對照組創(chuàng)面局部用0.9%氯化鈉溶液(N組);糖尿病組再隨機(jī)分成3組,糖尿病空白對照組(C組)創(chuàng)面局部外用0.9%氯化鈉溶液,糖尿病Ig G對照組(I組)創(chuàng)面局部外用兔Ig G,糖尿病RAGE干預(yù)組(R組)創(chuàng)面局部外用RAGE抗體。再于傷后第3日和第7日,給予相同處理。通過大體評估創(chuàng)面愈合情況;于傷后第1、3、7日切取創(chuàng)緣皮膚樣本,免疫組織化學(xué)染色標(biāo)記并計(jì)數(shù)創(chuàng)面中巨噬細(xì)胞數(shù)量,透射電子顯微鏡下觀察巨噬細(xì)胞超微結(jié)構(gòu)。結(jié)果·(1)傷后第14日,R組創(chuàng)面愈合面積百分比顯著高于C組和I組(均P=0.000)。(2)傷后第1日,N組和R組CD68+巨噬細(xì)胞數(shù)均顯著多于C組(均P=0.000);傷后第14日,N組和R組巨噬細(xì)胞數(shù)均顯著少于C組(均P=0.000)。(3)電子顯微鏡下對比創(chuàng)面組織的巨噬細(xì)胞形態(tài)及結(jié)構(gòu),存在明顯不同。結(jié)論·糖尿病創(chuàng)面環(huán)境中,RAGE效應(yīng)與創(chuàng)面巨噬細(xì)胞浸潤數(shù)量及形態(tài)的異常密切相關(guān),從而參與了糖尿病創(chuàng)面的難愈。
[Abstract]:Objective to investigate the effect of RAGE effect on macrophage infiltration during wound healing in diabetic mice after exogenous blockade of glycosylation end product receptor (rage).Methods 96 male 8-week-old C57BL/6J mice were randomly divided into diabetic group (n = 72) and normal control group (n = 24).The model of diabetic mice was induced by intraperitoneal injection of small dose streptozotocin (STZ), and the full-thickness skin defect wound model was made by sterilizing 9 mm diameter circular drilling on the back of mice.The normal control group was treated with 0.9% sodium chloride solution and the diabetic group was randomly divided into 3 groups: the control group was treated with 0.9% sodium chloride solution, and the control group was treated with 0.9% sodium chloride solution.Diabetic IGG control group (group I) wound local topical use of rabbit IGG, diabetic RAGE intervention group (group R) wound topical application of RAGE antibody.The same treatment is given on the 3rd and 7th day after injury.The wound healing was evaluated roughly, and the wound skin samples were taken on the 3rd and 7th day after injury. The number of macrophages in the wound was labeled with immunohistochemical staining, and the ultrastructure of macrophages was observed under transmission electron microscope.Results 1) the percentage of wound healing area in group R on the 14th day after injury was significantly higher than that in group C and group I (P0. 0000.000). The number of CD68 macrophages in group N and group R was significantly higher than that in group C (P0. 000), and the number of macrophages in group N and group R on the 14th day after injury was significantly higher than that in group C (P 0. 000).The number of macrophages in group C was significantly lower than that in group C (P0. 000, P < 0. 000), and the morphology and structure of macrophages in wound tissue were compared under electron microscope.There are obvious differences.Conclusion the rage effect in diabetic wound environment is closely related to the abnormal number and morphology of macrophage infiltration in the wound surface, thus participating in the refractory wound healing of diabetes mellitus.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院急診科;上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院上海燒傷研究所;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81272111,81671917)~~
【分類號】：R587.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 顧U喲，

本文編號：1766910