本文選題：高效液相色譜-電化學檢測　切入點：百草枯　出處：《分析測試學報》2017年09期

【摘要】：建立了單胺類神經(jīng)遞質的高效液相色譜-電化學檢測方法,探討了大蒜素對百草枯中毒小鼠腦組織內的單胺類神經(jīng)遞質及其代謝產(chǎn)物的影響。健康ICR小鼠隨機分為4組:正常對照(Con)組、大蒜素(DAS)組、百草枯染毒(PQ)組和大蒜素治療(PQ+DAS)組,每組6只。PQ組灌胃PQ 35 mg/kg建立中毒模型,Con組和DAS組灌服同體積生理鹽水。DAS組和PQ+DAS組小鼠于造模2、26、50 h后腹腔內各注射1次DAS(25 mg/kg),PQ組和正常組小鼠腹腔內注射等量無菌生理鹽水。造模12 h后處死小鼠。收集小鼠腦組織樣品并稱重,處理后進樣。色譜分析采用Acclaim 120 C18(150 mm×2.1 mm,3μm)色譜柱,以離子對緩沖鹽溶液-甲醇(93∶7,體積比)為流動相,流速為0.27 m L/min;電化學檢測器檢測,工作電壓為450 m V。結果表明,與正常組和DAS組比較,PQ組部分神經(jīng)遞質水平降低;DAS治療后能夠改變上述生化指標的變化;與PQ組相比,PQ+DAS組神經(jīng)遞質含量增高,差異均具有統(tǒng)計學意義(P0.05)。腦組織樣品中5-HT、DA、5-HIAA、DOPAC和HVA低、中、高3水平的加標回收率均為79.8%~112.1%,相對標準偏差(RSD)小于10%。實驗表明,大蒜素對百草枯中毒后小鼠體內含量降低的神經(jīng)遞質有明顯的上調作用。
[Abstract]:A high performance liquid chromatography-electrochemical detection method for monoamine neurotransmitters was established. The effects of allicin on monoamine neurotransmitters and their metabolites in the brain of paraquat poisoned mice were investigated. Healthy ICR mice were randomly divided into 4 groups: normal control group (Cona), allicin group (Das). Paraquat (PQQ) group and allicin treatment group (PQ DAS) group, Six mice in each group were given PQ35 mg/kg by intragastric administration of PQ35 mg/kg in each group. The mice in the Con group and the DAS group were given the same volume of normal saline. DAS and PQ DAS for 50 hours after the model was made. There was no equal amount of intraperitoneal injection of DAS(25 mg / kg PQ and normal mice in the control group and once a DAS(25 mg / kg group. The mice were killed after 12 hours of modeling. The brain tissue samples were collected and weighed. After treatment, the chromatographic analysis was performed on Acclaim 120C18C18mm 脳 2.1mm ~ (3 渭 m) column. The mobile phase consisted of ion-pair buffer salt solution and methanol (93: 7, volume ratio) at a flow rate of 0.27 mL / min. The working voltage was 450 MV by electrochemical detector. Compared with the normal group and the DAS group, the level of some neurotransmitters in the PQ group decreased and the changes of the above biochemical indexes were changed after treatment, and the neurotransmitter content in the PQ DAS group was higher than that in the PQ group, and that in the PQ DAS group was higher than that in the PQ group. The difference was statistically significant (P 0.05). The recoveries of 5-HTDDAN 5-HIAADOPAC and HVA in brain tissue samples were all 79.8% and 112.1, respectively, and the relative standard deviation (RSD) was less than 10%. Allicin significantly up-regulated the neurotransmitters of paraquat poisoning in mice.
【作者單位】： 河北醫(yī)科大學公共衛(wèi)生學院;
【基金】：國家自然科學基金資助項目(81573202)
【分類號】：O657;R595.4

【相似文獻】

相關期刊論文 前5條

1 趙燕燕;劉麗艷;韓媛媛;白潔;杜光玲;高茜;;高效液相色譜-熒光檢測法同時測定大鼠不同腦區(qū)中的8種單胺類神經(jīng)遞質[J];色譜;2011年02期

2 韋姍姍;陸雪萍;彭玲芳;屈會化;孫曄;王慶國;;HPLC-ECD法測定大鼠腦組織中單胺類神經(jīng)遞質的含量[J];中國藥房;2014年25期

3 李金昶;石晶;王廣;;大鼠腦組織中不同部位單胺類神經(jīng)遞質含量的HPLC測定[J];化學研究與應用;1993年02期

4 林麗,楊小鳳,李永,仇佩虹,曹旭妮,金利通;微滲析活體取樣-高效液相色譜電化學檢測法測定鼠腦中的單胺類神經(jīng)遞質[J];分析化學;2005年05期

5 張東明,付敏,馬萬云,陳瓞延;膠束電動毛細管色譜半導體激光誘導熒光測定單胺類神經(jīng)遞質[J];分析科學學報;2002年04期

相關碩士學位論文 前5條

1 武東;單胺類神經(jīng)遞質和尿酸色譜/光譜分析新方法的研究[D];安徽醫(yī)科大學;2015年

2 徐倩倩;基于功能化磁性納米粒子分離富集的尿樣單胺類神經(jīng)遞質分析研究[D];湖南師范大學;2015年

3 陳翼幫;單胺類神經(jīng)遞質高靈敏分析方法研究[D];湖南師范大學;2013年

4 蔣力維;功能化磁性粒子—高效液相色譜分離分析體液中的單胺類神經(jīng)遞質[D];湖南師范大學;2014年

5 王艷;制劑中γ-氨基丁酸和生物樣品中單胺類神經(jīng)遞質含量檢測方法的研究[D];河北大學;2013年

，

本文編號：1698703