本文選題：糖尿病性骨質(zhì)疏松癥　切入點(diǎn)：沙格列汀　出處：《山東醫(yī)藥》2017年28期

【摘要】：目的觀察沙格列汀對高糖環(huán)境下成骨細(xì)胞增殖、分化和凋亡的影響,為糖尿病性骨質(zhì)疏松患者選擇合理的降糖藥物提供理論依據(jù)。方法取成骨細(xì)胞MC3T3-E1置于a-MEM培養(yǎng)基中常規(guī)培養(yǎng)、傳代。取傳3代的對數(shù)生長期細(xì)胞,隨機(jī)分為正常對照組、高糖組、高糖聯(lián)合沙格列汀組,即刻更換a-MEM培養(yǎng)基,正常對照組更換為葡萄糖濃度5.5 mmol/L的培養(yǎng)液,高糖組更換為葡萄糖濃度30.0 mmol/L的培養(yǎng)液,高糖聯(lián)合沙格列汀組更換為葡萄糖濃度30.0 mmol/L+沙格列汀1μmol/L的培養(yǎng)液,繼續(xù)培養(yǎng)48 h。收集各組細(xì)胞,分別采用CCK-8法和流式細(xì)胞術(shù)檢測細(xì)胞增殖能力和細(xì)胞凋亡率,ELISA法檢測堿性磷酸酶(ALP)活性,qRT-PCR法檢測成骨細(xì)胞分化相關(guān)基因Ⅰ型膠原(COL-Ⅰ)、成骨特異性轉(zhuǎn)錄因子2(Runx2)、骨鈣蛋白(OCN)、骨橋蛋白(OPN)mRNA表達(dá)。結(jié)果與正常對照組比較,高糖組、高糖聯(lián)合沙格列汀組細(xì)胞增殖能力、ALP活性及成骨細(xì)胞分化相關(guān)基因COL-Ⅰ、Runx2、OCN、OPN mRNA相對表達(dá)量均明顯降低,細(xì)胞凋亡率均明顯升高(P均0.05);與高糖組比較,高糖聯(lián)合沙格列汀組細(xì)胞增殖能力、ALP活性和成骨細(xì)胞分化相關(guān)基因COL-Ⅰ、Runx2、OCN、OPN mRNA相對表達(dá)量均明顯升高,細(xì)胞凋亡率均明顯降低(P均0.05)。結(jié)論沙格列汀能在一定程度上逆轉(zhuǎn)高糖環(huán)境對小鼠成骨細(xì)胞增殖、分化和凋亡的影響,為糖尿病性骨質(zhì)疏松患者的降糖治療提供了理論依據(jù)。
[Abstract]:Objective to observe the effects of salgletine on the proliferation, differentiation and apoptosis of osteoblasts in high glucose environment, and to provide a theoretical basis for the selection of appropriate hypoglycemic drugs in diabetic osteoporosis patients. Three passages of logarithmic growth cells were randomly divided into normal control group, high glucose group, high glucose combined with salglutin group, and a-MEM medium was changed immediately. The normal control group was replaced with 5. 5 mmol/L glucose culture medium. The high glucose group was replaced with glucose concentration of 30.0 mmol/L, and the high glucose combined with salglutin group with glucose concentration of 30 mmol/L and 1 渭 mol/L. The cells of each group were collected and cultured for 48 hours. CCK-8 assay and flow cytometry were used to detect cell proliferation ability and apoptosis rate. Elisa was used to detect alkaline phosphatase (ALP) activity and qRT-PCR was used to detect osteoblast differentiation related gene type I collagen, osteoblast-specific transcription factor 2G Runx 2, bone. The expression of osteopontin, osteopontin, and osteopontin mRNA were compared with those in normal control group. In high glucose group and high glucose combined with salglutin group, the activity of ALP and the relative expression of osteoblast differentiation related gene COL- 鈪，

本文編號：1696547