本文選題：中暑　切入點：烏司他丁　出處：《解放軍醫(yī)學(xué)雜志》2017年04期

【摘要】：目的研究烏司他丁對重癥中暑所致小鼠心功能損害的保護(hù)作用,并進(jìn)一步探討其可能機(jī)制。方法 BALB/c小鼠20只按隨機(jī)數(shù)字表法分為常溫+生理鹽水組(Sham+NS組)、常溫+烏司他丁組(Sham+UTI組)、熱打擊+生理鹽水組(HS+NS組)、熱打擊+烏司他丁組(HS+UTI組)4組,每組5只。在熱打擊開始前腹腔注射烏司他丁10~5U/kg進(jìn)行預(yù)處理。常溫兩組置于環(huán)境溫度23.0±0.5℃條件下,熱打擊兩組置于高溫氣候動物培養(yǎng)箱(溫度36.5±0.5℃,濕度65.0%±2.0%)內(nèi),以直腸溫度(T_r)達(dá)42℃作為重癥中暑標(biāo)準(zhǔn)。達(dá)中暑標(biāo)準(zhǔn)后,移至常溫(23.0±0.5℃)進(jìn)行自然降溫處理,降溫6h后進(jìn)行心臟超聲檢測,分離心肌組織,進(jìn)行病理學(xué)觀察,Western blotting檢測總p38及磷酸化p38(p-p38)含量。結(jié)果在中暑熱打擊組中,HS+UTI組小鼠體溫達(dá)42℃的時間較HS+NS組明顯延長(P=0.044)。與Sham+NS組相比,HS+UTI組與HS+NS組動物心輸出量(CO)均明顯下降(P=0.017),炎癥損傷評分明顯升高(P0.001),p-p38/p38比值升高(P0.001),而HS+UTI組CO下降程度明顯低于HS+NS組(P=0.030),對應(yīng)的炎癥損傷評分(P0.001)及p-p38/p38比值(P=0.001)也均明顯低于HS+NS組。結(jié)論重癥中暑熱打擊可導(dǎo)致小鼠心肌炎性損傷和心功能障礙,而烏司他丁對重癥中暑小鼠心功能具有保護(hù)作用,可能與其下調(diào)p-p38水平,減輕心肌組織炎癥損傷有關(guān)。
[Abstract]:Objective to study the protective effect of ulinastatin on cardiac function damage induced by severe heat stroke in mice. Methods Twenty BALB/c mice were randomly divided into normal temperature NS group, normal temperature normal saline group, normal temperature ulinastatin group Sham UTI group, heat attack normal saline group HS-NS group and heat attack group. Ulinastatin group, HS UTI group, group 4, Five rats in each group were pretreated by intraperitoneal injection of ulinastatin 10~5U/kg before the beginning of the heat attack. The two groups were placed in the ambient temperature of 23.0 鹵0.5 鈩，

本文編號：1694728