本文選題：LMP-1基因　切入點(diǎn)：骨髓間充質(zhì)干細(xì)胞　出處：《山西醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:觀察攜帶LMP-1基因的骨髓間充質(zhì)干細(xì)胞對(duì)骨質(zhì)疏松大鼠的治療效果方法:健康三月齡雌性SD大鼠40只,由山西醫(yī)科大學(xué)動(dòng)物實(shí)驗(yàn)中心提供。隨機(jī)分成4組,每組各10只:1、卵巢切除組(A組):(1)股骨髓腔注射骨髓間充質(zhì)干細(xì)胞組(A1組);(2)股骨髓腔注射攜帶LMP-1基因的骨髓間充質(zhì)干細(xì)胞組(A2組);(3)股骨髓腔注射生理鹽水組(A3組);2、假手術(shù)組(B組)。3、各組大鼠在股骨髓腔注射相關(guān)物質(zhì)后第12周分別測(cè)量其體重,骨密度,外周血鈣、磷濃度,并進(jìn)行比較。之后再用空氣栓塞法處死大鼠,肉眼觀察各大鼠股骨大體標(biāo)本變化情況,HE染色法觀察股骨頭病理標(biāo)本組織學(xué)變化情況。結(jié)果:髓腔內(nèi)注射相關(guān)物質(zhì)12周時(shí)A1、A2、A3組大鼠骨質(zhì)各項(xiàng)指標(biāo)均較B組差,但是A組大鼠之間骨質(zhì)狀況A2優(yōu)于A1,A1優(yōu)于A3(P0.05)。結(jié)論:攜帶LMP-1基因的骨髓間充質(zhì)干細(xì)胞對(duì)骨質(zhì)疏松大鼠有一定的治療效果。
[Abstract]:Objective: To observe the therapeutic effect of cells carrying method osteoporosis rat mesenchymal stem LMP-1 gene in bone marrow: 3 month old healthy female SD rats of 40, provided by the animal experimental center of Shanxi Medical University. Randomly divided into 4 groups, 10 rats in each group: 1, ovariectomized group (A group): (1) femoral cavity injection of bone marrow mesenchymal stem cells group (A1 group); (2) femoral medullary cavity injection with LMP-1 gene in bone marrow mesenchymal stem cells group (A2 group); (3) femoral medullary cavity injection saline group (A3 group); 2, sham operation group (group B).3 group the rats in the femoral marrow cavity injection related substances were measured after twelfth weeks, weight, bone density, peripheral blood calcium, phosphorus concentration, and compared. Then the rats were killed by air embolism, each rat femur specimens of femoral head were observed by naked eye observation, pathological specimens histological changes of HE staining method results: intramedullary injection related substances At 12 weeks, all indexes of bone in A1, A2 and A3 groups were all worse than those in B group, but the bone status A2 in A group was superior to A1 and A1 was better than A3 (P0.05). Conclusion: bone marrow mesenchymal stem cells carrying LMP-1 gene have certain therapeutic effect on osteoporotic rats.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R580

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 梁長(zhǎng)生;向川;魏增永;侯慧銘;秦迎澤;衛(wèi)小春;;LMP-1基因慢病毒重組體對(duì)大鼠骨髓間充質(zhì)干細(xì)胞的增殖影響及其表達(dá)[J];中國(guó)骨傷;2013年12期

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本文編號(hào)：1689943