本文選題：氨基胍　切入點：破骨細(xì)胞　出處：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究不同濃度氨基胍對大鼠體外全骨髓誘導(dǎo)破骨細(xì)胞分化的影響,為骨質(zhì)疏松性疾病藥物治療提供實驗依據(jù)。方法:選用全骨髓誘導(dǎo)法培養(yǎng)破骨細(xì)胞(Osteoclast,OC),在培養(yǎng)當(dāng)天各組分別加入濃度為0mM、0.1mM、1mM、2.5mM的氨基胍(Aminoguanidine,AG),于第10天行抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,TRAP)染色,觀察TRAP陽性細(xì)胞數(shù)的差異;于培養(yǎng)第10天進(jìn)行掃描電鏡檢測骨陷窩發(fā)生的情況;于培養(yǎng)第10天收集細(xì)胞行RT-PCR檢測,測定OC特異性標(biāo)志物核因子-κB受體活化因子(Receptor activator of nuclear,RANK)和組織蛋白酶K(CathepsinK,CK)mRNA的表達(dá)量;于培養(yǎng)第10天收集細(xì)胞行Western Blot檢測OC活化信號通道蛋白P38和P65的表達(dá)量,及其測晚期糖基化終末產(chǎn)物(Advanced glycation end products,AGEs)和晚期糖基化終末產(chǎn)物受體(Receptor for advanced glycation end products,AGER)的表達(dá)量。結(jié)果:TRAP染色觀察示:不同濃度AG干預(yù)后,能夠起到促進(jìn)OC生成的作用,隨AG濃度增加,出現(xiàn)紅染細(xì)胞數(shù)逐漸增多,各組間差異有統(tǒng)計學(xué)意義,P0.05;骨吸收實驗示:不同濃度AG干預(yù)后,能夠促進(jìn)骨陷窩生成;RT-PCR檢測結(jié)果示:不同濃度AG干預(yù)后,能夠促進(jìn)OC形成,隨AG濃度的增加,OC特異標(biāo)志物RANK和CathepsinK的mRNA表達(dá)量逐漸增加,各組間差異有統(tǒng)計學(xué)意義;Western Blot檢測結(jié)果示:不同濃度AG干預(yù)后,能夠促進(jìn)OC形成,隨AG濃度的增加,P38和P65的表達(dá)量逐漸增加,同時檢測AGEs及其受體AGER的表達(dá)量逐漸降低,差異有統(tǒng)計學(xué)意義。結(jié)論:1.AG可以降低體外全骨髓誘導(dǎo)OC中AGEs和AGER的含量,在本實驗中起到了抑制AGEs及其受體AGER的作用,呈劑量依賴性。2.AG能夠增加體外全骨髓誘導(dǎo)OC的形成,在本實驗濃度范圍內(nèi)促進(jìn)了OC分化,呈劑量依賴性。
[Abstract]:Objective: to study the effects of different concentrations of aminoguanidine on osteoclast differentiation induced by whole bone marrow of rats in vitro. Methods: osteoclasts of osteoclasts were cultured by whole bone marrow induction method. Aminoguanidine Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Aminoguanidine (Aminoguanidine) Amin@@. The number of TRAP positive cells was observed, the incidence of bone lacuna was detected by scanning electron microscope on the 10th day of culture, and the RT-PCR was detected by RT-PCR on the 10th day of culture. The expression of nuclear factor 魏 B receptor activator activator of nuclear receptor activator activator of nuclear receptor (RANKK) and cathepsin Knk mRNA were measured, and the expression of OC-activated signal channel proteins P38 and p65 were detected by Western Blot on the 10th day of culture, and the expression of OC-activated signal channel protein p38 and p65 were detected by Western Blot. The expression of advanced glycation end products (ages) and late glycosylation end product receptor (Receptor for advanced glycation end products agers) were measured. The number of red stained cells increased gradually, and the difference was statistically significant (P 0.05). The results of bone absorption test showed that AG at different concentrations could promote the formation of bone lacunae by RT-PCR. With the increase of AG concentration, the mRNA expression of RANK and CathepsinK, the specific markers of OC, increased gradually. The results of Western Blot analysis showed that the different concentrations of AG could promote the formation of OC. With the increase of AG concentration, the expression of P38 and p65 increased, and the expression of AGEs and its receptor AGER decreased gradually. Conclusion: 1. AG can reduce the content of AGEs and AGER in bone marrow induced OC in vitro. In this experiment, AGEs and its receptor AGER were inhibited in a dose-dependent manner. 2. AG could increase the formation of OC induced by whole bone marrow in vitro, and promote the differentiation of OC in a dose-dependent manner.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R580

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曾艷平;楊超;楊紅軍;樊勇;吳子祥;劉斌;蘇菲;白博;張文強;桑宏勛;;阿司匹林體外抑制破骨細(xì)胞生成的分子機制研究[J];中國骨質(zhì)疏松雜志;2015年03期

2 趙帥;何愛詠;陶澄;;不同劑量骨保護(hù)素對破骨細(xì)胞內(nèi)一氧化氮生成及內(nèi)皮型一氧化氮合酶的影響[J];中國組織工程研究;2014年42期

3 張志偉;劉昌戎;廖金輝;沈為棟;;骨質(zhì)疏松癥與一氧化氮、內(nèi)皮素、降鈣素基因相關(guān)肽的關(guān)系研究進(jìn)展[J];中華實驗外科雜志;2014年09期

4 韋蓉梅;董麗平;吳紅慧;徐燕群;張健清;;強直性脊柱炎患者骨代謝生化指標(biāo)和RANKL-RANK-OPG系統(tǒng)表達(dá)之間的相關(guān)性[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2013年13期

5 張凌云;曲秀娟;劉云鵬;侯科佐;;PI3K/Akt信號通路在RANKL誘導(dǎo)的MCF-7乳腺癌細(xì)胞遷移中的作用[J];現(xiàn)代腫瘤醫(yī)學(xué);2012年02期

6 仲蕾蕾;楊冰;黃曉斌;孫元明;;OPG/RANKL/RANK系統(tǒng)在成骨細(xì)胞和破骨細(xì)胞相互調(diào)節(jié)中的作用[J];中國骨質(zhì)疏松雜志;2011年11期

7 陳建勇;王聰;王娟;曹禮榮;;MAPK信號通路研究進(jìn)展[J];中國醫(yī)藥科學(xué);2011年08期

8 劉中浩;高紅偉;邢德國;趙錫武;王若義;宮明智;;糖尿病性骨質(zhì)疏松模型雌激素、一氧化氮及轉(zhuǎn)化生長因子β1的變化[J];中國組織工程研究與臨床康復(fù);2011年11期

9 葛冬霞;李良;吳江;陳槐卿;;1,25(OH)_2維生素D_3誘導(dǎo)大鼠骨髓單核細(xì)胞形成破骨細(xì)胞[J];四川大學(xué)學(xué)報(醫(yī)學(xué)版);2007年02期

10 楊海平,王東,蔚晉斌,張登峰,武展雄;同種異體骨復(fù)合氨基胍對兔橈骨缺損愈合的影響[J];中國矯形外科雜志;2005年04期

，

本文編號：1684337