本文選題：心鈉素　切入點(diǎn)：哮喘　出處：《重慶醫(yī)學(xué)》2017年22期 　論文類型：期刊論文

【摘要】：目的探討外源性心鈉素對(duì)過敏性哮喘小鼠模型氣道炎癥的影響作用及其作用機(jī)制。方法選取SPF級(jí)BALBc小鼠48只,采用隨機(jī)數(shù)字表法分為對(duì)照組(等量生理鹽水干預(yù))、模型組(等量生理鹽水干預(yù))、實(shí)驗(yàn)組A(外源性心鈉素0.5μg/g)、實(shí)驗(yàn)組B(外源性心鈉素0.5μg/g+A71915)。模型組、實(shí)驗(yàn)組A、實(shí)驗(yàn)組B建模成功后給予對(duì)應(yīng)處理措施,在最后一次激發(fā)實(shí)驗(yàn)后24h,采集各組血清、支氣管肺泡灌洗液檢查炎癥因子,采用HE染色觀察肺組織病理變化,采用Western blot技術(shù)檢測(cè)肺組織中鋅指轉(zhuǎn)錄因子(GATA3)蛋白的表達(dá)水平。結(jié)果模型組小鼠的血液中EOS、淋巴細(xì)胞、中性粒細(xì)胞所占比例均高于對(duì)照組(P0.05),實(shí)驗(yàn)組A的血液中EOS、淋巴細(xì)胞、中性粒細(xì)胞所占比例均高于模型組、實(shí)驗(yàn)組B(P0.05);模型組小鼠的肺泡灌洗液中EOS、淋巴細(xì)胞、中性粒細(xì)胞計(jì)數(shù)、IL-6、TNF-α水平均高于對(duì)照組(P0.05),實(shí)驗(yàn)組A的肺泡灌洗液中EOS、淋巴細(xì)胞、中性粒細(xì)胞計(jì)數(shù)、IL-6、TNF-α水平均高于模型組、實(shí)驗(yàn)組B(P0.05);模型組小鼠的肺組織中GATA3蛋白表達(dá)水平均高于對(duì)照組(P0.05),實(shí)驗(yàn)組A肺組織中GATA3蛋白表達(dá)水平高于模型組、實(shí)驗(yàn)組B(P0.05)。結(jié)論外源性心鈉素會(huì)進(jìn)一步加劇過敏性哮喘小鼠模型的氣道炎癥反應(yīng),與激活炎性細(xì)胞、炎癥因子釋放及增強(qiáng)肺組織中GATA3蛋白表達(dá)有關(guān)。
[Abstract]:Objective to investigate the effect and mechanism of exogenous atrial natriuretic peptide (ANP) on airway inflammation in allergic asthmatic mice. Methods 48 SPF grade BALBc mice were selected. The rats were randomly divided into control group, model group, experimental group A (external atrial natriuretic peptide 0.5 渭 g / g) and experimental group B (external atrial natriuretic peptide 0.5 渭 g / g A71915). Experimental group A and group B were given corresponding treatment after successful modeling. 24 hours after the last exciting experiment, the serum and bronchoalveolar lavage fluid of each group were collected to examine the inflammatory factors, and the pathological changes of lung tissue were observed by HE staining. Western blot technique was used to detect the expression of zinc finger transcription factor (GATA3) protein in lung tissue. Results the percentage of EOS, lymphocytes and neutrophils in the blood of the model group was higher than that in the control group (P 0.05), and that in the blood of the experimental group A was higher than that of the control group. The percentage of neutrophils in the model group was higher than that in the model group, and the levels of EOS, lymphocytes and neutrophils in the alveolar lavage fluid of the model group were higher than those in the control group, and the levels of EOS and lymphocytes in the alveolar lavage fluid of the experimental group were higher than those in the control group. The level of IL-6 TNF- 偽 in the model group was higher than that in the model group, and the expression level of GATA3 protein in the lung tissue of the model group was higher than that of the control group (P 0.05), and the expression level of GATA3 protein in the lung tissue of the experimental group was higher than that of the model group. Conclusion exogenous atrial natriuretic peptide can further aggravate the airway inflammation in allergic asthma mice, which is related to the activation of inflammatory cells, the release of inflammatory factors and the enhancement of GATA3 protein expression in lung tissue.
【作者單位】： 天津市第五中心醫(yī)院呼吸內(nèi)科;
【基金】：天津市衛(wèi)生局資助基金項(xiàng)目(tj20150823)
【分類號(hào)】：R562.25

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