本文選題：GLP-1　切入點(diǎn)：高糖　出處：《福建醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的探討胰高血糖素樣肽(glucagon-like peptide-1,GLP-1)對高糖環(huán)境誘導(dǎo)兔胸主動脈內(nèi)皮細(xì)胞增殖、凋亡和熱休克蛋白(Heat shock Protein,HSP)70表達(dá)的影響。方法膠原酶消化法培養(yǎng)兔胸主動脈內(nèi)皮細(xì)胞,加入不同處理因素進(jìn)行分組,TUNEL法測定內(nèi)皮細(xì)胞的凋亡,BRDU法測定內(nèi)皮細(xì)胞的增殖,Western blot法測定內(nèi)皮細(xì)胞HSP70的表達(dá)水平。結(jié)果與對照組相比,高糖(33 mmol/L)培養(yǎng)24 h后兔胸主動脈內(nèi)皮細(xì)胞增殖下降、細(xì)胞凋亡率增加、細(xì)胞HSP70的表達(dá)水平增加(P0.05);高糖條件下加入GLP-1(10-8M)共培養(yǎng),與高糖組相比較,內(nèi)皮細(xì)胞增殖增加、細(xì)胞凋亡率減少、細(xì)胞HSP70表達(dá)水平減少(P0.05);加入GLP-1前,先用GLP-1受體抑制劑(10-7M)預(yù)處理,與高糖組相比,內(nèi)皮細(xì)胞的增殖、凋亡與HSP70表達(dá)則無明顯區(qū)別(P㧐0.05)。結(jié)論GLP-1通過GLP-1受體依賴途徑促進(jìn)高糖條件下兔胸主動脈內(nèi)皮細(xì)胞增殖,抑制細(xì)胞凋亡,減低高糖HSP70表達(dá)水平;GLP-1對延緩糖尿病血管病變可能與其降低高糖下內(nèi)皮細(xì)胞HSP70表達(dá)水平有關(guān)。
[Abstract]:Objective to investigate the effects of glucagon-like peptide-1 (GLP-1) on the proliferation, apoptosis and heat shock protein (HSP70) expression of rabbit thoracic aortic endothelial cells induced by high glucose. Methods the rabbit aortic endothelial cells were cultured by collagenase digestion. The apoptosis of endothelial cells was determined by Tunel method and the proliferation of endothelial cells was determined by BRDU method. The expression of HSP70 in endothelial cells was determined by Western blot assay. Results compared with the control group, the expression of HSP70 in endothelial cells was determined by TUNEL-mediated dUTP Nick end labeling (Tunel). After high glucose 33 mmol / L culture for 24 h, the proliferation of rabbit thoracic aorta endothelial cells decreased, the apoptosis rate increased, and the expression level of HSP70 increased in rabbit thoracic aorta, and GLP-1 + 10-8 M-1 was added in high glucose condition. Compared with high glucose group, the proliferation of endothelial cells increased. The rate of apoptosis was decreased and the expression of HSP70 was decreased (P 0.05). Before the addition of GLP-1, pretreatment with GLP-1 receptor inhibitor 10 ~ (-7) M, compared with high glucose group, the proliferation of endothelial cells, apoptosis and HSP70 expression were not significantly different. Conclusion GLP-1 can promote the proliferation of rabbit thoracic aortic endothelial cells and inhibit apoptosis through GLP-1 receptor dependent pathway. To reduce the level of high glucose HSP70 expression and the effect of GLP-1 on delaying diabetic vascular disease may be related to the decrease of HSP70 expression in endothelial cells under high glucose.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R587.2

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