本文關(guān)鍵詞： 脂肪細胞型脂肪酸結(jié)合蛋白 irisin 3-羧-4-甲-5-丙基呋喃戊酮酸 2型糖尿病 胰島β細胞功能　出處：《南方醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：背景最近幾年來,越來越多的研究表明,肥胖與細胞因子中的FABP4密切有關(guān),而且肥胖相關(guān)的胰島素抵抗也與FABP4有著緊密的關(guān)聯(lián)。Irisin是新近發(fā)現(xiàn)的一種細胞因子,在體內(nèi)和體外的研究中發(fā)現(xiàn),運動誘導(dǎo)irisin的大量分泌,在一定程度上改善胰島素抵抗。然而,脂肪細胞因子FABP4與irisin之間又有怎樣的關(guān)聯(lián)呢?另外,CMPF在2型糖尿病一級親屬這一高危人群中的血清濃度尚不清楚,并且關(guān)于CMPF與人體胰島β細胞分泌功能的研究尚少。因此,本研究旨在探討血清irisin和FABP4之間的關(guān)聯(lián),以及不同糖代謝狀態(tài)下CMPF的濃度和其與糖脂代謝和胰島β細胞分泌功能之間的關(guān)聯(lián)。目的1.探討在社區(qū)人群中血清FABP4和irisin水平,與糖脂代謝的關(guān)聯(lián)以及兩者的相關(guān)性;2.檢測在2型糖尿病患者的血清中細胞因子FABP4與irisin的水平,探討這兩個指標(biāo)之間的關(guān)聯(lián);3.探討不同糖代謝狀態(tài)下CMPF的濃度和其與糖脂代謝和胰島β細胞分泌功能之間的關(guān)聯(lián)。方法招募上海市奉賢區(qū)40-60歲近半年內(nèi)體檢身體健康志愿者111名,其中男性56名,女性55名。另招募上海市奉賢區(qū)新診斷T2DM患者99名,糖尿病前期患者99名,T2DM—級親屬99名,糖耐量正常者99名。按照2003年ADA的診斷標(biāo)準(zhǔn)來診斷糖尿病前期和T2DM。采用酶聯(lián)免疫吸附試驗ELISA法檢測血清中細胞因子FABP4、CMPF及irisin的表達水平。結(jié)果1.血清FABP4濃度與性別相關(guān)(r=0.348,P0.001),女性明顯高于男性。矯正性別后的偏相關(guān)分析提示血清FABP4水平與舒張壓(r=0.270,P=0.005)、體脂百分比(r=0.251,P=0.012)呈正相關(guān),與骨骼肌質(zhì)量(r=-0.335,P=0.001)、irisin(r=-0.203,P=0.033)呈負相關(guān)。2.與糖耐量正常組相比較,血清CMPF濃度在糖尿病前期(P=0.019)、新診斷T2DM組(P0.001)和T2DM—級親屬組(P0.001)顯著升高。雙相關(guān)分析表明血清CMPF水平與體重指數(shù)(r=-0.129,P=0.037)、甘油三酯(r=-0.203,P=0.001)、早相胰島素分泌指數(shù)(r=-0.150,P=0.015)、晚相胰島素分泌指數(shù)(r=-0.127,P=0.041)呈負相關(guān)。結(jié)論1.在我國40-60歲中年社區(qū)人群中,血清irisin水平與FABP4呈獨立相關(guān)。血清irisin表達水平與糖脂代謝以及腎功能好壞的關(guān)系十分密切。新診斷T2DM患者血清irisin與FABP4呈獨立負相關(guān),提示irisin可能具有拮抗FABP4的作用。2.血清CMPF水平在糖尿病前期、T2DM及T2DM—級親屬中濃度明顯升高。血清CMPF水平與胰島β細胞分泌功能呈獨立相關(guān),隨著CMPF水平的升高β細胞分泌功能逐漸下降。因此,本研究表明血清CMPF濃度與糖代謝紊亂相關(guān),CMPF濃度的升高可能是導(dǎo)致高血糖及β細胞功能障礙的相關(guān)因素。
[Abstract]:Background in recent years, more and more studies have shown that obesity is closely related to FABP4 in cytokines, and obesity related insulin resistance is closely related to FABP4. Irisin is a newly discovered cytokine. In vivo and in vitro, it has been found that exercise induces a large amount of irisin secretion and improves insulin resistance to some extent. However, what is the relationship between the adipocyte factor FABP4 and irisin? In addition, the serum concentration of CMPF in first-degree relatives of type 2 diabetes is not clear, and there are few studies on the relationship between CMPF and 尾 -cell secretion in human pancreatic islets. Therefore, the purpose of this study is to explore the relationship between serum irisin and FABP4. And the relationship between the concentration of CMPF and the glucose and lipid metabolism and the secretion function of islet 尾 cells under different glucose metabolism. Objective 1. To explore the levels of serum FABP4 and irisin in community population. The relationship between glucose and lipid metabolism and the correlation between them. 2. The levels of cytokines FABP4 and irisin in the serum of patients with type 2 diabetes mellitus were detected. To explore the correlation between these two indexes. To study the concentration of CMPF in different glucose metabolism state and its relationship with glucose and lipid metabolism and 尾 -cell secretory function. Methods physical examination was conducted in Fengxian District of Shanghai within nearly half a year of 40-60 years old. 111 healthy volunteers, Among them, 56 were males and 55 were females. 99 newly diagnosed T2DM patients were recruited from Fengxian District of Shanghai, 99 relatives of pre-diabetic patients with T2DM- grade, 99 subjects with normal glucose tolerance were diagnosed as prediabetic and T2DMaccording to the diagnostic criteria of ADA in 2003. The expression levels of cytokine FABP4CMPF and irisin in serum were detected by enzyme linked immunosorbent assay (ELISA). 1. Serum FABP4 concentration and sex. The results of partial correlation analysis showed that the serum FABP4 level was positively correlated with diastolic blood pressure (DBP) 0.270, and the percentage of body fat was 0.251% (P < 0.012). There was a negative correlation with the skeletal muscle mass, ru -0.335P ~ (0.001) and ~ (0.203) ~ (P ~ (0.033)). 2. Compared with the normal glucose tolerance group, there was a negative correlation between the two groups, and compared with the normal glucose tolerance group, there was no significant difference between the two groups. Serum CMPF level was significantly higher in prediabetic patients than in newly diagnosed T2DM group (P 0.001) and T2DM-grade relatives group (P 0.001). Double correlation analysis showed that serum CMPF level and body mass index (BMI) were 0.129%, 0.129% and 0.037%, triglyceride level -0.203P0.001, early phase insulin secretion index (r = -0.150), late phase insulin secretion, and late phase insulin secretion, respectively. Conclusion 1. In the middle aged community population of 40-60 years old, there is a negative correlation between r and 0.127 (P = 0.041). Serum irisin level was independently correlated with FABP4, serum irisin expression level was closely related to glycolipid metabolism and renal function. Serum irisin was negatively correlated with FABP4 in newly diagnosed T2DM patients. The results suggest that irisin may antagonize FABP4. 2. Serum CMPF level is significantly increased in prediabetic patients with T2DM and T2DM- grade relatives. Serum CMPF level is independent of islet 尾 -cell secretory function. As the level of CMPF increased, 尾 -cell secretory function decreased gradually. Therefore, this study suggests that the increase of serum CMPF concentration and glucose metabolism disorder may be the related factors of hyperglycemia and 尾 -cell dysfunction.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R587.1

【參考文獻】

相關(guān)期刊論文 前2條

1 王文娟;王思揚;劉潔瓊;張偉光;董哲毅;張利;朱晗玉;蔡廣研;陳香美;;糖尿病腎病血清鳶尾素水平的變化及其影響因素的研究[J];中國中西醫(yī)結(jié)合腎病雜志;2016年05期

2 黃衛(wèi)東;劉毅;辛超;杜朝升;張君毅;宋延彬;陶凌;;Irisin對2型糖尿病小鼠血糖的影響及機制[J];心臟雜志;2014年04期

相關(guān)碩士學(xué)位論文 前1條

1 潘紅霞;PCOS患者血清irisin水平及其與胰島素抵抗的關(guān)系研究[D];重慶醫(yī)科大學(xué);2015年

，

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