本文關(guān)鍵詞： miR-a 細(xì)胞周期阻滯 纖維化 糖尿病腎病　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：目的研究糖尿病腎病(DN)患者腎組織miR-451a表達(dá)量與腎功能、腎纖維化的關(guān)系,探討其臨床應(yīng)用價(jià)值。方法對(duì)DN患者進(jìn)行腎活檢,活檢組織進(jìn)行病理分級(jí)的同時(shí)提取RNA通過(guò)q PCR分析miR-451a、p15INK4b(p15)、E-caherin、α-SMA的表達(dá)變化;提取總蛋白分析各指標(biāo)蛋白水平變化;并分析尿素、肌酐、尿酸、尿微量白蛋白/肌酐比(ACR)的改變。結(jié)果共收集DN患者46例,年齡39~64歲,男28例,女18例。病理分級(jí)為I級(jí)2例,Ⅱa級(jí)6例,Ⅱb級(jí)8例,Ⅲ級(jí)26例,IV級(jí)4例。DN組腎組織miR-451a表達(dá)量為對(duì)照組的0.2倍(P0.05),p15的mRNA表達(dá)量為5.3倍(P0.05)。隨纖維化加重,miR-451a、E-caherin水平逐步下降,p15、α-SMA逐步升高(趨勢(shì)分別為42.21、35.36、31.43、45.96,P0.001)。miR-451a水平與p15、α-SMA、尿素、ACR呈負(fù)相關(guān)(r=-0.88,-0.83,-0.87,-0.86,P0.05),與E-cadherin呈正相關(guān)(r=0.86,P0.05)。P15的蛋白表達(dá)量(I級(jí)為對(duì)照組的2.62倍,Ⅱa級(jí)3.00倍,Ⅱb級(jí)3.76倍,Ⅲ級(jí)3.88倍,IV級(jí)4.58倍)、α-SMA的蛋白水平(升至2.24倍,2.82倍,3.00倍,3.10倍,3.64倍),而E-cadherin依次降至0.96,0.61,0.53,0.45,0.29。結(jié)論 DN腎纖維化過(guò)程中腎miR-451a呈低表達(dá),與周期阻滯、EMT、腎功能存在相關(guān)性,可能成為纖維化新的評(píng)價(jià)指標(biāo)及治療靶標(biāo)。
[Abstract]:Objective to study the relationship between renal miR-451a expression and renal function and renal fibrosis in patients with diabetic nephropathy (DN). RNA was extracted from the biopsy tissues with pathological grading and E-caherin was analyzed by Q PCR analysis of miR-451ap15INK4bP15p15p15cherin. Changes of 偽 -SMA expression; Total protein was extracted to analyze the changes of protein levels. The changes of urea, creatinine, uric acid and urinary albumin / creatinine ratio (ACRs) were analyzed. Results there were 46 DN patients aged 3964 years and 28 males. 18 cases were female. Pathological grading was grade I in 2 cases, grade 鈪，

本文編號(hào)：1488982