本文關(guān)鍵詞： 糖尿病 型 骨質(zhì)疏松 骨密度 椎間盤退化 組織工程 組織構(gòu)建 型糖尿病 鏈脲佐菌素 椎間盤退變 河北省自然科學(xué)基金　出處：《中國(guó)組織工程研究》2017年12期 　論文類型：期刊論文

【摘要】：背景:高脂飼料配合低劑量鏈脲佐菌素誘導(dǎo)大鼠2型糖尿病模型是目前常用的2型糖尿病動(dòng)物模型,目前尚無(wú)此模型對(duì)椎間盤結(jié)構(gòu)影響的相關(guān)報(bào)道。椎體終板硬化是造成椎間盤退變的重要因素之一。目的:評(píng)估高脂飼料配合低劑量鏈脲佐菌素誘導(dǎo)2型糖尿病大鼠模型是否適用于2型糖尿病相關(guān)椎間盤退變研究。方法:32只3月齡雌性SPF級(jí)SD大鼠隨機(jī)分為4組,即假手術(shù)組、雙側(cè)卵巢切除組、糖尿病組、雙側(cè)卵巢切除+糖尿病組,每組8只。分別進(jìn)行雙側(cè)卵巢切除術(shù)和/或高脂飼料配合低劑量鏈脲佐菌素處理。監(jiān)測(cè)各組大鼠體質(zhì)量、血糖及糖耐量,鏈脲佐菌素處理后8周檢測(cè)骨密度后處死動(dòng)物,取L_(5-6)節(jié)段脫鈣后正中矢狀位切片行VG染色并進(jìn)行椎間盤退變?cè)u(píng)分,分析椎間盤高度和終板厚度。結(jié)果與結(jié)論:(1)糖尿病組和雙側(cè)卵巢切除+糖尿病組大鼠空腹血糖、隨機(jī)血糖分別顯著高于假手術(shù)組和雙側(cè)卵巢切除組(P0.05),糖尿病組和雙側(cè)卵巢切除+糖尿病組大鼠胰島素敏感性分別顯著低于假手術(shù)組和雙側(cè)卵巢切除組(P0.05);(2)雙側(cè)卵巢切除組L_4-L_6椎體骨密度顯著低于假手術(shù)組(P0.05),糖尿病組和雙側(cè)卵巢切除+糖尿病組L_5-L_6椎體骨密度均顯著低于假手術(shù)組(P0.05);(3)雙側(cè)卵巢切除組和雙側(cè)卵巢切除+糖尿病組L_5-L_6椎間盤退變?cè)u(píng)分分別顯著高于假手術(shù)組和糖尿病組(P0.05);雙側(cè)卵巢切除+糖尿病組表現(xiàn)與雙側(cè)卵巢切除組相仿,可見髓核細(xì)胞軟骨化生及黏液變性,其評(píng)分結(jié)果顯著高于假手術(shù)組和糖尿病組(P均0.05),但與雙側(cè)卵巢切除組相比差異未見顯著性意義;(4)與假手術(shù)組相比,雙側(cè)卵巢切除組和雙側(cè)卵巢切除+糖尿病組椎間盤高度明顯降低(P0.05);4組間終板厚度差異無(wú)顯著性意義;(5)綜上所述,雖然高脂飼料配合低劑量鏈脲佐菌素誘導(dǎo)2型糖尿病大鼠模型具備糖尿病特征表現(xiàn),但未觀察到其間盤組織較正常大鼠有顯著改變,可能不適于2型糖尿病相關(guān)椎間盤退變研究。
[Abstract]:Background: high fat diet with low dose of streptozotocin induced rat model of type 2 diabetes mellitus is a common animal model of type 2 diabetes, there is no reports about the effect of this model on the intervertebral disc structure at present. Vertebral endplate sclerosis is one of the important factors of intervertebral disc degeneration. Objective: To evaluate the high fat diet with low the dose of streptozotocin induced type 2 diabetic rat model is suitable for the study of type 2 diabetes related intervertebral disc degeneration. Methods: 32 male 3 month old female SPF SD rats were randomly divided into 4 groups: sham operation group, ovariectomy group, ovariectomy + diabetes group, diabetes group, n = 8 only. Respectively were subjected to bilateral ovariectomy and / or high-fat diet combined with low dose of streptozotocin. The rats body weight monitoring, blood glucose and glucose tolerance, STZ bone density after 8 weeks of treatment detection after the death of animal, L_ (5-6) day Section of decalcified sagittal sections were stained by VG staining and disc degeneration score, analysis of disc height and endplate thickness. Results and conclusion: (1) diabetic group and ovariectomy + fasting blood glucose in diabetic rats, random blood glucose were significantly higher than that of sham group and bilateral ovariectomy group (P0.05), the diabetic group and ovariectomy + insulin sensitivity in diabetic rats were significantly lower than that of sham group and ovariectomized group (P0.05); (2) ovariectomy group L_4-L_6 vertebral bone mineral density was significantly lower than that in the sham operation group (P0.05), diabetes mellitus group and ovariectomized + diabetes group L_5-L_6 vertebral bone mineral density significantly compared with sham operation group (P0.05); (3) ovariectomized group and ovariectomy + diabetes group L_5-L_6 intervertebral disc degeneration scores were significantly higher than those of sham operation group and diabetes group (P0.05); ovariectomy + diabetes Disease group performance and ovariectomy group were nucleus pulposus cells cartilage metaplasia and mucoid degeneration, the scores were significantly higher than that in sham operation group and diabetic group (P 0.05), but with the ovariectomy group compared to the difference was not significant; (4) compared with the sham operation group, ovariectomized group and ovariectomy + diabetes group of intervertebral disc height significantly decreased (P0.05); there was no significant difference in endplate thickness between the 4 groups; (5) to sum up, although the high fat diet with low dose of streptozotocin induced type 2 diabetic rat model with diabetes features, but not observed in disc compared with normal rats had a significant change, may not be suitable for study of type 2 diabetes related intervertebral disc degeneration.

【作者單位】： 華北理工大學(xué);
【基金】：河北省自然科學(xué)基金資助項(xiàng)目(H2013209257) 唐山市科技創(chuàng)新團(tuán)隊(duì)培養(yǎng)計(jì)劃(15130208C)~~
【分類號(hào)】：R-332;R587.1
【正文快照】： 文章快速閱讀:高脂飼料配合低劑量鏈脲佐菌素誘導(dǎo)2型糖尿病大鼠模型是否適用于2型糖尿病相關(guān)椎間盤退變研究分組:干預(yù):材料:假手術(shù)組;分別進(jìn)行雙側(cè)卵巢切除術(shù)32只SD大鼠隨機(jī)分為4雙側(cè)卵巢切除組;和/或高脂飼料配合低劑量組,每組8只。糖尿病組;鏈脲佐菌素處理。雙側(cè)卵巢切除+糖

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