本文關(guān)鍵詞： RNA干擾 Toll樣受體 哮喘 支氣管 凋亡　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2017年09期 　論文類型：期刊論文

【摘要】：為了研究RNA干擾基因表達(dá)對(duì)哮喘小鼠支氣管上皮細(xì)胞凋亡的影響,本研究擬通過RNA干擾技術(shù)降低Toll樣受體表達(dá),檢測其是否對(duì)緩解哮喘小鼠的支氣管上皮細(xì)胞凋亡發(fā)揮作用。本實(shí)驗(yàn)將30只小鼠,雌雄各半,隨機(jī)分為對(duì)照組、哮喘組以及干預(yù)組3組。哮喘造模成功后,原代培養(yǎng)各組小鼠的支氣管上皮細(xì)胞,4~8代后進(jìn)行si RNA干擾。通過RT-PCR以及TUNEL的方法分別檢測小鼠細(xì)胞內(nèi)Toll樣受體的基因表達(dá)量和細(xì)胞凋亡情況。結(jié)果顯示,哮喘的小鼠Toll樣受體的表達(dá)明顯升高,支氣管上皮細(xì)胞凋亡比例也比較高,與對(duì)照組小鼠進(jìn)行比較,兩種指標(biāo)均有統(tǒng)計(jì)學(xué)差異;而si RNA進(jìn)行干擾后,相較于哮喘組,Toll樣受體的表達(dá)量明顯下降;且凋亡細(xì)胞的比例也明顯下降,與哮喘組進(jìn)行比較,兩組間有明顯的統(tǒng)計(jì)學(xué)差異。本研究在小鼠模型中驗(yàn)證了用RNA干擾技術(shù)對(duì)Toll樣受體基因表達(dá)的干預(yù)作用,并且也證明此技術(shù)能明顯改善哮喘小鼠的病理表型,是非常有前景的臨床治療方法,為臨床推廣提供了較為可靠的基礎(chǔ)實(shí)驗(yàn)證據(jù)。
[Abstract]:In order to study the effect of RNA interference gene expression on the apoptosis of bronchial epithelial cells in asthmatic mice, we intend to reduce the expression of Toll like receptor by RNA interference technique. In this experiment, 30 mice, half male and half female, were randomly divided into three groups: control group, asthma group and intervention group. Primary culture of bronchial epithelial cells of each group of mice. Si RNA interference was performed after 8 passages. The expression of Toll like receptor gene and apoptosis in mouse cells were detected by RT-PCR and TUNEL. The expression of Toll like receptor and the percentage of apoptosis of bronchial epithelial cells in asthmatic mice were significantly higher than those in control mice. The expression of Toll-like receptor was significantly decreased after si RNA interference compared with asthmatic group. The percentage of apoptotic cells also decreased significantly, compared with asthma group. There was significant statistical difference between the two groups. This study demonstrated the effect of RNA interference on the expression of Toll like receptor gene in mouse model. It is also proved that this technique can obviously improve the pathological phenotype of asthmatic mice. It is a very promising clinical treatment and provides more reliable experimental evidence for clinical popularization.
【作者單位】： 湘南學(xué)院附屬醫(yī)院;武漢大學(xué)人民醫(yī)院呼吸內(nèi)科;
【基金】：湘南學(xué)院 武漢大學(xué)人民醫(yī)院資助
【分類號(hào)】：R562.25
【正文快照】： *通訊作者,s_sfang@163.comEffect of RNA Interference Gene Expression on Apoptosis of Epithelial Cellin Asthmatic MiceYan Jianhui1Huang Lijuan1Liu Jianping1Chen Hongbin2Luo Gaomei1Wang Wenting1Chen Yanbin1*1 Affiliated Hospital of Xiangnan University,Chenz

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