本文關(guān)鍵詞： Th細(xì)胞 Th細(xì)胞 哮喘 肺功能　出處：《中國免疫學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:探討Th17、Th9細(xì)胞水平在支氣管哮喘患者中的表達(dá)及其臨床意義。方法:選取67例哮喘患者為研究對象,30例健康體檢者為對照組,根據(jù)全球哮喘防治倡議(GINA)將哮喘患者分為間歇與輕度持續(xù)組20例,中度持續(xù)組25例和重度持續(xù)組22例。收集患者臨床資料,完成哮喘控制測試(ACT)評分、肺功能和呼出氣一氧化氮(Fe NO)值測定,流式細(xì)胞儀檢測外周血PBMC中CD3~+CD8~-IL-17~+、CD3~+CD8~-IL-9~+細(xì)胞水平。結(jié)果:與健康對照組相比,哮喘患者Th17、Th9細(xì)胞水平均明顯升高(P0.05);中重度哮喘患者Th17及Th9細(xì)胞水平顯著高于間歇與輕度哮喘組(P0.05),而間歇與輕度哮喘組患者與健康對照組比較,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。哮喘患者外周血Th17及Th9細(xì)胞的表達(dá)均與FeNO值正相關(guān)(r值依次為0.501、0.438;P0.05),與ACT評分、肺功能FEV1、FEV1/FVC及PEF負(fù)相關(guān)(r值依次為-0.441、-0.362;-0.307、-0.377;-0.419、-0.411;-0.428、-0.324;P0.05),同時(shí)Th17與Th9細(xì)胞在哮喘患者外周血中的表達(dá)率正相關(guān)(r值為0.443,P0.05)。結(jié)論:Th17、Th9細(xì)胞在哮喘患者中高表達(dá),具有協(xié)同致炎作用,且與癥狀嚴(yán)重程度相關(guān),提示Th17及Th9細(xì)胞對哮喘患者病情評估有潛在的參考價(jià)值。
[Abstract]:Objective: to investigate the expression of Th17 Th9 cells in patients with bronchial asthma and its clinical significance. Methods: 67 asthmatic patients were selected as the study object and 30 healthy persons as the control group. According to the Global Initiative for the Prevention and treatment of Asthma (GINA), asthmatic patients were divided into intermittent and mild persistent group (n = 20), moderate persistent group (n = 25) and severe persistent group (n = 22). The asthma control test was completed with ACT-score, pulmonary function and exhalation nitric oxide (no) value. The levels of CD3 ~ CD8 + -IL-17 ~ + CD3 ~ CD8 + -IL-9 ~ ~ cells in peripheral PBMC were detected by flow cytometry. Results: compared with the healthy control group. Th17 Th 9 cells were significantly increased in asthmatic patients (P 0.05). The levels of Th17 and Th9 cells in moderate and severe asthmatic patients were significantly higher than those in intermittent and mild asthma groups, while those in intermittent and mild asthma groups were significantly higher than those in healthy controls. There was no significant difference (P 0.05). The positive correlation between the expression of Th17 and Th9 cells in peripheral blood and FeNO value was 0.501 and 0.438 respectively. P0.05, and ACT score, FEV1 / FEV1 / FVC and PEF negative correlation r values were -0.441- 0.362; -0.307, -0.377; -0.419 + -0.411; -0.428 + -0.324; The positive correlation between the expression rate of Th17 and Th9 cells in the peripheral blood of asthmatic patients was 0.443P0.050.Conclusion: 1. The high expression of Th9 cells in asthmatic patients has synergistic inflammatory effect and is related to the severity of symptoms. It suggests that Th17 and Th9 cells have potential reference value for the evaluation of asthma patients' condition.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院呼吸與危重癥醫(yī)學(xué)科;
【基金】：國家臨床重點(diǎn)�？茖ｍ�(xiàng)資助(民辦醫(yī)政函[2012]649號)
【分類號】：R562.25
【正文快照】： (1)本文受國家臨床重點(diǎn)�？茖ｍ�(xiàng)資助(民辦醫(yī)政函[2012]649號)。支氣管哮喘是由多種細(xì)胞(如氣道上皮細(xì)胞、成纖維細(xì)胞、嗜酸性粒細(xì)胞、中性粒細(xì)胞、T淋巴細(xì)胞、肥大細(xì)胞和平滑肌細(xì)胞等)及其細(xì)胞組分參與的一種慢性氣道炎癥性疾病。免疫功能異常是其重要的發(fā)病機(jī)制[1]。輔助性T

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