發(fā)布時間：2018-01-29 11:39

  本文關(guān)鍵詞： 唑來膦酸 聚乳酸-羥基乙酸聚合物 β-磷酸三鈣 去勢大鼠 骨缺損　出處：《中國骨質(zhì)疏松雜志》2017年08期 　論文類型：期刊論文

【摘要】：目的唑來膦酸、聚乳酸-羥基乙酸聚合物、β-磷酸三鈣(ZA、PLGA、β-TCP)復(fù)合支架對去勢大鼠股骨干骺端骨缺損的修復(fù)作用。方法雌性SD大鼠經(jīng)過雙側(cè)去卵巢手術(shù)后飼養(yǎng)3個月建立骨質(zhì)疏松模型,隨后在大鼠雙側(cè)股骨干骺端建立直徑為3 mm圓形骨缺損,上述大鼠隨機分為4組;分別置入ZA、PLGA支架,β-TCP支架和ZA、PLGA、β-TCP復(fù)合支架,不置入支架材料的為對照組。術(shù)后12周取材,通過Micro-CT掃描重建和病理組織學(xué)評價三組支架材料的成骨作用。結(jié)果術(shù)后觀察發(fā)現(xiàn)空白對照組骨缺損未修復(fù),骨缺損斷端硬化,而其他3組骨缺損均有不同程度修復(fù)。植入骨質(zhì)疏松大鼠體內(nèi)12周后,三組材料隨著材料的降解均有新生骨長入,且三組新骨生成率均顯著優(yōu)于對照組(P0.05),缺損區(qū)域都有較高BV/TV、Tb.Th、Tb.N、Conn.D、骨礦化沉積率(MAR)和較低的Tb.Sp,其中以ZA、PLGA、β-TCP組最高。Micro-CT和病理組織學(xué)結(jié)果提示ZA、PLGA、β-TCP組骨修復(fù)效果較ZA、PLGA與β-TCP組更好。結(jié)論 ZA、PLGA、β-TCP復(fù)合支架具有明顯促進去勢大鼠股骨干骺端骨缺損修復(fù)的作用。
[Abstract]:Objective Zoledronic acid, polylactic acid-glycolic acid polymer, 尾 -tricalcium phosphate ZAAPLGA. Methods Osteoporosis model was established in female SD rats after bilateral ovariectomy. Then the 3 mm circular bone defect was established in the bilateral femoral metaphysis of rats. The above rats were randomly divided into 4 groups. ZAA-PLGA stents, 尾 -TCP stents and ZAA-PLGA, 尾 -TCP composite stents were implanted respectively. The osteogenesis of the three groups of scaffolds was evaluated by Micro-CT scan reconstruction and histopathology. Results the bone defects in the blank control group were not repaired and the broken end of the bone defects were sclerosis. The other three groups of bone defects were repaired in varying degrees. After 12 weeks of implantation into the body of osteoporosis rats, the three groups of materials with the degradation of materials with the new bone growth into the three groups. The rate of new bone formation in the three groups was significantly higher than that in the control group (P 0.05), and there was a higher BV / TVT Tb. Tb. Bone mineralization deposition rate (Mar) and lower Tb.Sp.among them, ZAL-PLGA, 尾 -TCP group was the highest. Micro-CT and histopathological results suggested ZAA-PLGA. The bone repair effect of 尾 -TCP group was better than that of ZAA-PLGA group and 尾 -TCP group. Conclusion ZAA-PLGA is better than 尾 -TCP group. 尾-TCP composite scaffolds can significantly promote the repair of femoral metaphyseal bone defects in ovariectomized rats.
【作者單位】： 濟南市第四人民醫(yī)院骨科;
【分類號】：R580
【正文快照】： 骨質(zhì)疏松癥(osteoporosis,OP)是因多種原因引發(fā)的一組以骨量減少、骨脆性增加及骨微結(jié)構(gòu)變化為基礎(chǔ)病理的代謝性骨病變疾病,病人臨床主要表現(xiàn)為腰背疼痛、身長縮短、駝背及骨折并發(fā)癥。隨著世界經(jīng)濟的快速發(fā)展、人類生活水平及衛(wèi)生事業(yè)水平巨大提高,人們的年齡也在不斷的提高,，

本文編號：1473331