本文關(guān)鍵詞： 食物過敏 Trek 腸道屏障功能 益生菌　出處：《重慶醫(yī)學(xué)》2017年22期 　論文類型：期刊論文

【摘要】：目的探討產(chǎn)丁酸的益生菌酪酸梭菌通過調(diào)節(jié)腸上皮細(xì)胞雙孔鉀通道Trek1的表達(dá)對過敏小鼠腸道屏障功能的影響。方法建立小鼠食物過敏模型,ELISA法、流式細(xì)胞儀檢測相關(guān)指標(biāo)驗證造模效果,尤斯室檢測小腸組織通透性變化,Western blot及免疫熒光法檢測Na6ve組、Saline組、SIT組、SIT/SB組、SB組、SIT/CB組、CB組、SIT/CB/Spadin組小鼠空腸組織中Trek1的表達(dá);Transwell系統(tǒng)中使用T84細(xì)胞建立單層上皮細(xì)胞層,分別暴露于過敏介質(zhì),qRT-PCR和Western blot檢測Trek1mRNA和蛋白的表達(dá);分別使用生理鹽水、SIT、SIT/SB、SB、SIT/CB、CB、SIT/CB/Spadin不同療法對過敏小鼠進(jìn)行治療,了解小鼠腸道Trek1的表達(dá)情況、腸道屏障功能及過敏反應(yīng)指標(biāo)的變化情況。結(jié)果相對于對照組,食物過敏小鼠小腸Trek1蛋白表達(dá)水平顯著下降,腸黏膜通透性顯著升高,差異有統(tǒng)計學(xué)意義(P0.05);T84細(xì)胞暴露于過敏介質(zhì)后,Trek1mRNA和蛋白的表達(dá)顯著下降(P0.05),但預(yù)先加入p38抑制劑可以拮抗這種改變;SIT治療、酪酸梭菌和丁酸鈉單獨應(yīng)用均可以提高食物過敏小鼠腸道Trek1的表達(dá),減輕過敏反應(yīng)(P0.05),但SIT與酪酸梭菌或丁酸鈉的聯(lián)合應(yīng)用效果更加顯著(與SIT組相比P0.05),并且可以顯著降低小腸黏膜通透性,改善腸道屏障功能(P0.05)。結(jié)論丁酸鈉或者產(chǎn)丁酸的益生菌酪酸梭菌可以通過提高Trek1的表達(dá)來恢復(fù)過敏小鼠的腸道屏障功能,減輕過敏反應(yīng),并且加強(qiáng)SIT治療的效果。
[Abstract]:Objective to investigate the effects of Clostridium caseinate, a probiotics producing butyric acid, on intestinal barrier function in allergic mice by regulating the expression of double-pore potassium channel Trek1 in intestinal epithelial cells. ELISA and flow cytometry were used to test the effect of model making. The changes of intestinal permeability were detected by Eus chamber. The changes of intestinal permeability were detected by Western blot and immunofluorescence were used to detect the Na6ve group. The expression of Trek1 in jejunum of Saline / sit / sit / SB / SB / CB / CB / CB group. T84 cells were used in the Transwell system to establish a single layer of epithelial cells, which were exposed to allergy mediators respectively. QRT-PCR and Western blot were used to detect the expression of Trek1mRNA and protein. The allergic mice were treated with different treatments of normal saline SITS / SBT / SBT / CBT / CBST / CB/ Spadin. Results compared with the control group, the expression of Trek1 protein in the small intestine of mice with food allergy was significantly lower than that in the control group. The permeability of intestinal mucosa was significantly increased (P 0.05). The mRNA and protein expression of Trek1 in T84 cells decreased significantly after exposure to allergy mediators, but p38 inhibitor could antagonize the changes. SIT treatment, Clostridium caseinate and sodium butyrate alone can increase the expression of Trek1 in the intestine of food allergy mice and alleviate the allergic reaction (P0.05). However, the combination of SIT with Clostridium caseinate or sodium butyrate was more effective (compared with SIT group, P 0.05), and could significantly reduce intestinal mucosal permeability. Conclusion Sodium butyrate or butyric acid-producing probiotics Clostridium caseate can restore the intestinal barrier function of allergic mice by increasing the expression of Trek1 and alleviate the allergic reaction. And enhance the effect of SIT treatment.
【作者單位】： 鄭州大學(xué)第五附屬醫(yī)院消化科;
【基金】：國家自然科學(xué)基金資助項目(81370494) 國家973計劃前期研究專項(2011CB512006) 河南省科技創(chuàng)新杰出人才基金(134200510022) 鄭州市創(chuàng)新型科技人才隊伍建設(shè)工程基金(131PLJRC656)
【分類號】：R593.1
【正文快照】： 近年來,過敏性疾病的發(fā)病率逐漸增高,其中約有8%的兒童和5%的成年人發(fā)生過針對某些特定食物抗原的食物過敏。食物過敏是一種Th2細(xì)胞因子驅(qū)動的免疫紊亂疾病,癥狀包括腹痛、嘔吐、蕁麻疹、腹瀉等,嚴(yán)重者可發(fā)生過敏性休克。腸道屏障功能對于維持機(jī)體的免疫穩(wěn)態(tài)有著重要的意義,研

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