本文關鍵詞：年齡、肥胖及糖尿病對胰島素樣生長因子結(jié)合蛋白2在脂肪組織中表達的影響　出處：《吉林大學》2016年碩士論文　論文類型：學位論文

  更多相關文章： 肥胖 衰老 胰島素樣生長因子結(jié)合蛋白2 內(nèi)臟脂肪 mRNA

【摘要】：目的:肥胖是一種復雜的、由多種因素共同作用導致的慢性非傳染性疾病。肥胖可以導致體內(nèi)多種代謝紊亂而增加許多疾病的患病風險,大量流行病學調(diào)查顯示肥胖人群比正常人群更容易出現(xiàn)葡萄糖耐量減低和胰島素抵抗。目前研究發(fā)現(xiàn),衰老、肥胖和胰島素抵抗是共存的。而胰島素樣生長因子結(jié)合蛋白2(IGFBP2)作為由白色脂肪組織分泌的脂肪因子,可以有效預防高脂飲食誘導的肥胖及年齡相關性胰島素抵抗的發(fā)生與發(fā)展。但是,IGFBP2在發(fā)生胰島素抵抗的組織中的表達情況尚不明確。因此,此項研究的目的在于明確IGFBP2在內(nèi)臟白色脂肪組織的表達情況是否受到肥胖、胰島素抵抗及衰老的調(diào)控,及其與血液循環(huán)中IGFBP2濃度的關系。材料與方法:全部小鼠均選取雄性,分別選取4月齡、12月齡和24月齡的C57BL6小鼠各12只;4月齡ob/ob,db/db和野生型小鼠各6只;4月齡高糖高脂飲食誘導的肥胖小鼠(HFD-ob)及正常飲食對照的C57BL6小鼠各6只。全部小鼠禁食8小時后斷頸處死,并立即留取其血液(心臟穿刺)和內(nèi)臟白色脂肪組織及皮下白色脂肪組織,分別使用離心后冷藏及直接液氮冷藏的方式存儲標本。脂肪組織提取RNA,反轉(zhuǎn)錄為c DNA進行real-time PCR檢測m IGFBP2的表達情況,并通過ELISA試劑盒對相應小鼠IGFBP2的血漿水平進行測定。使用方差分析、t檢驗及單因素相關性回歸分析進行數(shù)據(jù)統(tǒng)計,所有數(shù)據(jù)使用SPSS17版本軟件進行分析,P0.05認為有統(tǒng)計學意義。結(jié)果:(1)年齡對IGFBP2在脂肪組織中表達的影響:研究發(fā)現(xiàn)非肥胖小鼠組,與4月齡的小鼠比較,12月齡和24月齡小鼠的內(nèi)臟白色脂肪組織中IGFBP2 m RNA的表達明顯減少(P0.05),而在皮下脂肪組織無明顯差別;(2)肥胖和糖尿病對IGFBP2在脂肪組織表達的影響:與同為4月齡的正常飲食小鼠組比較,HFD-ob小鼠內(nèi)臟脂肪組織IGFBP2 m RNA表達水平顯著降低,與野生型小鼠相比,ob/ob、db/db小鼠內(nèi)臟脂肪組織IGFBP2 m RNA表達水平顯著降低,而IGFBP2在皮下脂肪組織中的表達不受肥胖和糖尿病的影響,此差異在皮下脂肪組織未獲得;(3)IGFBP2在上述小鼠血液中的檢測結(jié)果與其在內(nèi)臟白色脂肪組織中的表達情況呈現(xiàn)高度一致性。結(jié)論:IGFBP2的基因轉(zhuǎn)錄調(diào)節(jié)具有組織特異性,其在內(nèi)臟白色脂肪組織及血液循環(huán)中的表達受年齡、肥胖及糖代謝異常的影響,且具有高度一致性。這一結(jié)果暗示年齡和糖脂代謝異常對調(diào)控IGFBP2的表達具有重要的作用。
[Abstract]:Objective: obesity is a complex chronic non-communicable disease caused by multiple factors. Obesity can lead to multiple metabolic disorders in the body and increase the risk of many diseases. A large number of epidemiological studies have shown that obese people are more likely than normal to develop impaired glucose tolerance and insulin resistance. Obesity and insulin resistance coexist, and insulin-like growth factor-binding protein 2IGFBP2is a fatty factor secreted by white adipose tissue. It can effectively prevent the occurrence and development of obesity and age-related insulin resistance induced by high fat diet. However, the expression of IGFBP2 in insulin-resistant tissues is not clear. The aim of this study was to determine whether the expression of IGFBP2 in visceral white adipose tissue is regulated by obesity, insulin resistance, and aging. Materials and methods: all the male mice were selected, 12 C57BL6 mice of 4 months old and 24 months old C57BL6 mice were selected. Each of the 4 month old ob-obo db- db and wild-type mice was composed of 6 mice and 6 wild-type mice. Four months old high-sugar high-fat diet induced obesity mice (HFD-ob) and normal diet control C57BL6 mice each 6. All the mice were killed after 8 hours fasting. The blood (heart puncture), visceral white adipose tissue and subcutaneous white adipose tissue were collected immediately and stored in centrifugation and direct liquid nitrogen storage respectively. RNA was extracted from adipose tissue. The expression of m IGFBP2 was detected by real-time PCR with c DNA reverse transcription. The plasma level of mouse IGFBP2 was measured by ELISA kit, and the data were analyzed by ANOVA t test and single factor correlation regression analysis. All data is analyzed using SPSS17 version software. Results the effect of age on the expression of IGFBP2 in adipose tissue: the study found that the non-obese mice group was compared with the 4-month-old mice. The expression of IGFBP2 m RNA in white adipose tissue of mice aged 12 months and 24 months decreased significantly, but there was no significant difference in subcutaneous adipose tissue. (2) the effect of obesity and diabetes on the expression of IGFBP2 in adipose tissue: compared with normal diet mice of 4 months of age. The expression of IGFBP2 m RNA in visceral adipose tissue of HFD-ob mice was significantly lower than that in wild type mice. The expression of IGFBP2 m RNA in visceral adipose tissue of db/db mice was significantly decreased, while the expression of IGFBP2 in subcutaneous adipose tissue was not affected by obesity and diabetes. This difference was not obtained in subcutaneous adipose tissue. 3). The results of IGFBP2 in the blood of the above mice were highly consistent with their expression in visceral white adipose tissue. Conclusion the gene transcriptional regulation of 1: IGFBP2 has tissue specificity. Its expression in visceral white adipose tissue and blood circulation is affected by age, obesity and abnormal glucose metabolism. These results suggest that age and abnormal glucose and lipid metabolism play an important role in regulating the expression of IGFBP2.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R589.2;R587.1

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