【摘要】：目的:通過(guò)檢測(cè)大鼠腎缺血再灌注損傷模型后不同時(shí)段腎功能、血清血管內(nèi)皮生長(zhǎng)因子的表達(dá)量及腎臟病理,了解血管內(nèi)皮生長(zhǎng)因子在腎缺血再灌注損傷發(fā)生后的功能及川芎嗪的干預(yù)作用。方法:制作大鼠腎缺血再灌注損傷模型,采用夾心雙抗免疫酶聯(lián)吸附法測(cè)定不同組別血清血管內(nèi)皮生長(zhǎng)因子的表達(dá),與腎臟病理、腎功能的相關(guān)指標(biāo)進(jìn)行分析。結(jié)果:1、缺血再灌注組和川芎嗪組大鼠術(shù)后12和24小時(shí)血鉀、血尿素氮高于同時(shí)段的假手術(shù)組,且有統(tǒng)計(jì)學(xué)意義(P0.05)。2、川芎嗪組大鼠血鉀、尿素氮均低于同時(shí)段缺血再灌注組,且有統(tǒng)計(jì)學(xué)意義。(P0.05)3、缺血再灌注組、川芎嗪組組內(nèi)12小時(shí)和24小時(shí)血鉀、尿素氮之間差異有統(tǒng)計(jì)學(xué)意義。(P0.05)4、缺血再灌注組和川芎嗪組各時(shí)段血肌酐平均值均高于同時(shí)段假手術(shù)組;川芎嗪組血肌酐各時(shí)段平均值小于缺血再灌注組,12小時(shí)內(nèi)血肌酐平均值上升小于缺血再灌注組。5、缺血再灌注組、川芎嗪組的大鼠腎組織Paller氏評(píng)分都分別明顯大于假手術(shù)組;川芎嗪組的腎小管Paller氏評(píng)分分別小于同時(shí)段缺血再灌注組。6、缺血再灌注組和川芎嗪大鼠組術(shù)后12小時(shí)組血清血管內(nèi)皮生長(zhǎng)因子的表達(dá)均高于同時(shí)段的假手術(shù)組;川芎嗪組大鼠術(shù)后12小時(shí)血清VEGF表達(dá)低于同時(shí)段缺血再灌注組。結(jié)論:1、脊柱旁開(kāi)約0.5cm、背雙側(cè)肋弓下緣縱行約1.5cm切口夾閉雙側(cè)大鼠腎動(dòng)靜脈60分鐘后恢復(fù)灌注可以成功制作大鼠腎缺血再灌注損傷模型,且此方法模型操作時(shí)簡(jiǎn)便易行,成功率高;2、缺血再灌注組和川芎嗪組術(shù)后12小時(shí)血清血管內(nèi)皮生長(zhǎng)因子表達(dá)量即有升高趨勢(shì),血管內(nèi)皮生長(zhǎng)因子可以作為早期腎臟缺血損傷的檢驗(yàn)指標(biāo);3、川芎嗪可以降低腎缺血再灌注損傷發(fā)生后的血鉀、肌酐、尿素氮的數(shù)值、腎小管損傷評(píng)分,并起到保護(hù)腎臟結(jié)構(gòu)和功能的作用。
[Abstract]:Objective: to investigate the expression of serum vascular endothelial growth factor (VEGF) and renal pathology in different periods after renal ischemia reperfusion injury in rats. To investigate the function of vascular endothelial growth factor (VEGF) after renal ischemia reperfusion injury and the intervention of ligustrazine. Methods: the rat model of renal ischemia-reperfusion injury was established. The expression of vascular endothelial growth factor (VEGF) in different groups of serum was measured by sandwich double immunosorbent assay (Elisa), and the correlation indexes of renal pathology and renal function were analyzed. Results: 1. The blood potassium and blood urea nitrogen of rats in ischemia reperfusion group and ligustrazine group were significantly higher than those in sham operation group at 12 and 24 hours postoperatively (P0.05). Urea nitrogen was lower than that of ischemia reperfusion group at the same time, and had statistical significance. (P0.05) 3, Ischemia-reperfusion group, Ligustrazine group 12 hours and 24 hours blood potassium, urea nitrogen difference was statistically significant. (P0.05) 4, The mean value of serum creatinine in ischemia-reperfusion group and ligustrazine group was higher than that in sham-operation group at the same time. The mean value of serum creatinine in ligustrazine group was lower than that in ischemia-reperfusion group, and the mean value of creatinine in 12 hours was lower than that in ischemia-reperfusion group. The Paller's score of renal tissue in ligustrazine group was significantly higher than that in sham operation group. The Paller's score of renal tubules in ligustrazine group was lower than that in ischemia-reperfusion group at the same time. 6. The expression of vascular endothelial growth factor (VEGF) in serum of ligustrazine group and ligustrazine group 12 hours after operation was higher than that in sham-operation group at the same time. The expression of serum VEGF in ligustrazine group was lower than that in ischemia reperfusion group 12 hours after operation. Conclusion: 1. The model of renal ischemia-reperfusion injury in rats can be made successfully after 60 minutes of bilateral renal arteriovenous occlusion by longitudinal 1.5cm incision along the lower margin of dorsal bilateral costal arch. The method is simple and easy to operate and has a high success rate. 2. The expression of vascular endothelial growth factor (VEGF) in serum of ischemia reperfusion group and ligustrazine group increased 12 hours after operation. Vascular endothelial growth factor could be used as an index of early renal ischemia injury. 3. Ligustrazine could reduce the values of serum potassium, creatinine, urea nitrogen, renal tubular injury score and protect the renal structure and function after renal ischemia-reperfusion injury.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R692

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