發(fā)布時(shí)間：2018-07-12 12:57

  本文選題：三氧化二砷 + 腎母細(xì)胞瘤�。� 參考：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年02期

【摘要】：目的觀察三氧化二砷(As2O3)對體外人腎母細(xì)胞瘤SK-NEP-1細(xì)胞株增殖和凋亡的影響。方法應(yīng)用0、1.25、2.5、5、10μmol/L As2O3(A、B、C、D、E組)作用SK-NEP-1細(xì)胞24 h。采用MTT比色法檢測不同濃度As2O3對SK-NEP-1細(xì)胞的增殖抑制率;流式細(xì)胞儀檢測凋亡率;ELISA法檢測SK-NEP-1細(xì)胞Bcl-2水平變化;Caspase 3試劑盒檢測細(xì)胞Caspase 3活性。結(jié)果 A、B、C、D、E組細(xì)胞增殖抑制率分別為(3.25±1.05)%、(11.19±2.27)%、(20.72±2.21)%、(27.24±0.57)%、(32.25±2.15)%,細(xì)胞凋亡率分別為(5.12±0.29)%、(13.42±1.03)%、(23.70±0.97)%、(32.44±0.82)%、(40.22±1.31)%,各組間差異均具有統(tǒng)計(jì)學(xué)意義(均P0.05)。B、C、D、E組Bcl-2蛋白表達(dá)水平均低于A組(均P0.05),Caspase 3活性均高于A組(均P0.05)。結(jié)論 As2O3能明顯抑制腎母細(xì)胞瘤細(xì)胞的增殖,并進(jìn)一步誘導(dǎo)其凋亡,其抗腫瘤機(jī)制可能與下調(diào)Bcl-2表達(dá)及激活Caspase 3有關(guān)。
[Abstract]:Objective to observe the effect of arsenic trioxide (as _ 2O _ 3) on proliferation and apoptosis of human nephroblastoma cell line SK-NEP-1 in vitro. Methods SK-NEP-1 cells were treated with 10 渭 mol / L as _ 2O _ 3 10 渭 mol 路L ~ (2 +) C _ 2O _ 3 for 24 h. MTT assay was used to detect the proliferation inhibition rate of SK-NEP-1 cells at different concentrations of as _ 2O _ 3, and flow cytometry (FCM) was used to detect the apoptosis rate and the level of Bcl-2 in SK-NEP-1 cells. Caspase 3 activity was detected by Caspase 3 kit. 緇撴灉 A,B,C,D,E緇勭粏鑳?yōu)澧瀸D栨姂鍒剁巼鍒嗗埆涓，

本文編號(hào)：2117230