本文選題：E-cadherin + N-cadherin　； 參考：《蘭州大學》2014年碩士論文

【摘要】：目的：研究上皮型鈣粘蛋白(E-cadherin)和神經(jīng)元型鈣粘蛋白(N-cadherin)在良性前列腺增生組織及前列腺癌組織中的表達情況,以探討E-cadherin和N-cadherin在前列腺癌發(fā)生發(fā)展過程中的作用及意義。 方法：采用免疫組織化學法(SP法)檢測51例前列腺癌組織和47例良性前列腺增生組織中E-cadherin和N-cadherin的表達,根據(jù)半定量積分法判斷陽性結(jié)果。根據(jù)前列腺癌的病理分化等級及臨床分期,采用x2檢驗對E-cadherin、 N-cadherin與前列腺癌的關(guān)系進行統(tǒng)計分析。采用RT-PCR方法檢測E-cadherin mRNA以及N-cadherin mRNA在前列腺增生和前列腺癌中的表達,并進行統(tǒng)計學分析。 結(jié)果：1.在良性前列腺增生組織中,E-cadherin陽性表達率為87.23%,N-cadherin的陽性表達率為4.26%；在前列腺癌組織中,E-cadherin陽性表達率為31.37%, N-cadherin的陽性表達率為86.27%。與前列腺增生組織中的表達結(jié)果相比較,E-cadherin和N-cadherin在前列腺癌中的表達具有顯著差異(P0.01)。2. E-cadherin在前列腺癌組織中的高分化組、中分化組以及低分化組的陽性表達率分別為75.00%、46.67%以及10.71%; N-cadherin在前列腺癌組織中的高分化組、中分化組以及低分化組的陽性表達率分別為37.5%、80.00%及96.43%。E-cadherin和N-cadherin在前列腺癌高、中、低分化組各組之間進行兩兩比較,均有差異(P0.05)。3. E-cadherin在前列腺癌臨床分期A、B、C、D各期的陽性表達率分別為100.00%、75.00%、36.84%及4.55%, N-cadherin在前列腺癌臨床分期A、B、C、D各期的陽性表達率分別為50.00%、37.50%、57.89%及86.36%。4. E-cadherin mRNA和N-cadherin mRNA的表達,在前列腺癌組織中的Gleason評分低分化組與前列腺增生組織相比較具有顯著性差異(P0.01); Gleason評分中分化組與前列腺增生組相比較具有統(tǒng)計學意義(P0.05)；而Gleason評分高分化組與前列腺增生組相比較無顯著性差異。 結(jié)論：1. E-cadherin與前列腺癌的惡性程度及臨床分期呈負相關(guān)。2.N-cadherin與前列腺癌的惡性程度及臨床分期呈正相關(guān)。
[Abstract]:Objective: to investigate the expression of E-cadherin and N-cadherin in benign prostatic hyperplasia (BPH) and prostate cancer, and to explore the role and significance of E-cadherin and N-cadherin in the development of prostate cancer. Methods: the expression of E-cadherin and N-cadherin in 51 cases of prostate cancer and 47 cases of benign prostatic hyperplasia were detected by immunohistochemical method (SP method). The relationship between E-cadherin, N-cadherin and prostate cancer was statistically analyzed by using x2 test according to the grade of pathological differentiation and clinical stage of prostate cancer. The expression of E-cadherin mRNA and N-cadherin mRNA in prostatic hyperplasia and prostate cancer were detected by RT-PCR. The result is 1: 1. The positive expression rate of E-cadherin was 4.26 in benign prostatic hyperplasia, 31.37 in prostate cancer and 86.27 in N-cadherin. The expression of E-cadherin and N-cadherin in prostate cancer was significantly different from that in benign prostatic hyperplasia (P0.01). The positive expression rates of E-cadherin in prostate cancer tissues were 75.00%, 46.67% and 10.71%, respectively, and the positive rates of N-cadherin in prostate cancer tissues were 75.00% and 10.71%, respectively. The positive expression rates of E-cadherin and N-cadherin in moderately differentiated group and poorly differentiated group were 37.50.000% and 96.43km. E-cadherin and N-cadherin were significantly higher than those in poorly differentiated group (P0.05). The positive expression rate of E-cadherin in clinical stage of prostate cancer was 100.000.The positive rate of N-cadherin in clinical stage of prostate cancer was 50.0057.50% and 86.360.45%, respectively. The positive expression rate of E-cadherin in clinical stage of prostate cancer was 57.89% and 86.36.4.The positive expression rate of E-cadherin in clinical stage of prostate cancer was 57.89% and 86.36.4.The positive expression rate of E-cadherin in clinical stage of prostate cancer was 57.89% and 86.36.4. The expression of E-cadherin mRNA and N-cadherin mRNA in prostate cancer was significantly higher than that in prostatic hyperplasia (P0.01), and there was significant difference in Gleason score between differentiated group and prostatic hyperplasia group (P0.05). There was no significant difference in Gleason score between high differentiation group and benign prostatic hyperplasia group. Conclusion 1. E-cadherin was negatively correlated with the malignant degree and clinical stage of prostate cancer. 2.N-cadherin was positively correlated with the malignant degree and clinical stage of prostate cancer.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.25

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