本文選題：膀胱癌 + PTEN　； 參考：《蚌埠醫(yī)學(xué)院》2017年碩士論文

【摘要】：背景:膀胱癌是我國泌尿外科臨床上最常見的腫瘤之一,并且90%以上為移行細(xì)胞癌,目前的一些研究表明,第10號染色體缺失的磷酸酶和張力蛋白同源基因(Phosphatase and tension homology deleted from chromosome 10,PTEN)和自噬基因Beclin1在膀胱移行細(xì)胞癌的發(fā)生、發(fā)展起著重要的作用,PTEN和Beclin1可能是膀胱移行細(xì)胞癌的重要標(biāo)志物和潛在的治療靶點(diǎn)。目的:檢測膀胱癌組織、正常膀胱組織中PTEN和Beclin1的表達(dá)情況并結(jié)合膀胱癌臨床病理特征,分析二者在膀胱癌發(fā)生發(fā)展中的作用及相關(guān)性。方法:收集患者膀胱癌標(biāo)本52例(經(jīng)病理復(fù)檢均為移行細(xì)胞癌),另取正常膀胱組織標(biāo)本10例作為對照,用免疫組織化學(xué)檢測PTEN和Beclin1在上述組織標(biāo)本中的表達(dá)情況,結(jié)合膀胱移行細(xì)胞癌相關(guān)臨床病理因素,研究二者在膀胱移行細(xì)胞癌組織中的表達(dá)與膀胱移行細(xì)胞癌其他生物學(xué)特點(diǎn)之間的關(guān)系。結(jié)果:1.膀胱移行細(xì)胞癌組織和正常膀胱組織中PTEN的陽性表達(dá)率,二者分別為53.84%和100%,差異顯著,有統(tǒng)計(jì)學(xué)意義(P0.05);2.膀胱移行細(xì)胞癌組織和正常膀胱組織中Beclin1的陽性表達(dá)率,二者分別為59.62%和100%,差異顯著,有統(tǒng)計(jì)學(xué)意義(P0.05);3.PTEN的表達(dá)與組織病理學(xué)分級、TNM分期明顯相關(guān)(P0.05),而與患者性別、年齡無明顯相關(guān)(P0.05);Beclin1的表達(dá)與組織病理學(xué)分級、腫瘤TNM分期明顯相關(guān)(P0.05),而與患者性別、年齡無明顯相關(guān)(P0.05);4.在膀胱移行細(xì)胞癌中,PTEN和Beclin1的陽性表達(dá)率呈明顯的正相關(guān)(r=0.378,P0.05)。結(jié)論:1.與正常膀胱組織相比,PTEN基因在膀胱移行細(xì)胞癌組織中表達(dá)水平明顯降低,且與腫瘤組織病理學(xué)分級、TNM分期明顯相關(guān)(P0.05)。2.與正常膀胱組織相比,Beclin1基因在膀胱移行細(xì)胞癌組織中表達(dá)水平明顯降低,且與腫瘤組織病理學(xué)分級、TNM分期明顯相關(guān)(P0.05)。3.膀胱移行細(xì)胞癌組織中PTEN與Beclin1的表達(dá)存在正相關(guān)性;臨床上聯(lián)合檢測這兩種標(biāo)志物在膀胱腫瘤中的表達(dá)對腫瘤的診斷具有一定的意義。
[Abstract]:Background: bladder cancer is one of the most common tumors in urology in China, and more than 90% are transitional cell carcinoma. Phosphatase and tension homology deleted from chromosome 10PTEN and autophagy Beclin1 are present in transitional cell carcinoma of bladder. PTEN and Beclin1 may be important markers and potential therapeutic targets for bladder transitional cell carcinoma. Objective: to detect the expression of PTEN and Beclin1 in bladder cancer and normal bladder tissues, and to analyze the role of PTEN and Beclin1 in the carcinogenesis and development of bladder cancer. Methods: the expression of PTEN and Beclin1 were detected by immunohistochemistry in 52 cases of bladder cancer (transitional cell carcinoma) and 10 cases of normal bladder tissue as control. Combined with the clinicopathological factors related to transitional cell carcinoma of bladder, the relationship between their expression in transitional cell carcinoma of bladder and other biological characteristics of transitional cell carcinoma of bladder was studied. The result is 1: 1. The positive expression rate of PTEN in transitional cell carcinoma of bladder and normal bladder was 53.84% and 100, respectively. The difference was significant (P0.05). The positive expression rates of Beclin1 in transitional cell carcinoma of bladder and normal bladder were 59.62% and 100, respectively. The difference was significant (P0.05). 3. The expression of PTEN was significantly correlated with the TNM stage of histological grade (P0.05), but with the sex of the patients. There was no significant correlation between age (P0.05) and tumor TNM stage (P0.05), but there was no significant correlation between age and age (P0.05). The positive expression rates of PTEN and Beclin1 in transitional cell carcinoma of bladder were positively correlated (r = 0.378P 0.05). Conclusion 1. Compared with normal bladder tissue, the expression level of PTEN gene in bladder transitional cell carcinoma (TCC) was significantly lower than that in normal bladder tissue, and was significantly correlated with TNM stage of tumor histopathology (P0.05). Compared with normal bladder tissue, the expression of Beclin1 gene was significantly decreased in transitional cell carcinoma of bladder, and was significantly correlated with TNM stage of tumor histopathology (P0.05). There is a positive correlation between the expression of PTEN and Beclin1 in bladder transitional cell carcinoma, and the detection of the expression of these two markers in bladder cancer has some significance in the diagnosis of bladder cancer.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.14

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