本文選題：前列腺腫瘤 + 前列腺癌細(xì)胞　； 參考：《第二軍醫(yī)大學(xué)學(xué)報》2017年06期

【摘要】：目的制備一種用于對人前列腺癌PC3細(xì)胞進(jìn)行體外檢測的粒細(xì)胞靶向介導(dǎo)的磁性-熒光納米探針。方法制備以Fe_3O_4為核、表面包被SiO_2和異硫氰酸羅丹明的具有磁性且攜帶紅色熒光的納米材料,將其與正常人外周血粒細(xì)胞按不同比例混合,分別孵育不同時間以檢測納米材料對粒細(xì)胞的毒性。選擇最佳比例將納米材料和粒細(xì)胞體外結(jié)合,得到粒細(xì)胞靶向介導(dǎo)的磁性-熒光納米探針;將PC3細(xì)胞與正常人全血細(xì)胞分別以不同比例混合,加入上述探針,于熒光顯微鏡下觀察探針的靶向情況。結(jié)果在實驗所用的濃度和作用時間范圍內(nèi),制備的納米探針對粒細(xì)胞存活率無明顯影響。鏡下可見納米探針在PC3細(xì)胞周圍富集,形成"花瓣樣"結(jié)構(gòu),而全血細(xì)胞周圍無探針富集。結(jié)論本研究制備的磁性-熒光納米探針不會對粒細(xì)胞本身產(chǎn)生明顯毒性,利用粒細(xì)胞對腫瘤細(xì)胞的生物靶向作用可使納米探針有效地靶向檢測腫瘤細(xì)胞。
[Abstract]:Objective to prepare a magnetic fluorescent nano probe mediated by granulocyte targeting for human prostate cancer PC3 cells in vitro, and to prepare nano materials with magnetic and red fluorescence with SiO_2 and isothiocyanate Luo Danming as the core of Fe_3O_4 and mixed with normal human peripheral blood granulocytes in a different proportion. In order to detect the toxicity of nanomaterials to granulocytes at different time, the optimum proportion was chosen to combine the nanomaterial and granulocyte in vitro, and the magnetic fluorescent nano probe mediated by granulocyte targeting was obtained, and the PC3 cells were mixed with the normal human whole blood cells in different proportions, and the probe was observed under the fluorescence microscope. The results showed that there was no obvious effect on the survival rate of granulocytes in the concentration and time range used in the experiment. The nano probe was enriched around PC3 cells and formed a petal like structure under the microscope. There was no probe enrichment around the whole blood cells. It has obvious toxicity to granulocytes. The biological targeting effect of granulocytes on tumor cells can make nanomaterials effectively detect tumor cells.
【作者單位】： 同濟(jì)大學(xué)醫(yī)學(xué)院生物醫(yī)學(xué)工程與納米科學(xué)研究院;維克森林大學(xué)醫(yī)學(xué)院病理系;辛辛那提大學(xué)工程與應(yīng)用科學(xué)學(xué)院機(jī)械與材料工程系;
【基金】：國家自然科學(xué)基金(31671029) 2016國家大學(xué)生創(chuàng)新訓(xùn)練項目(1500107123/001)~~
【分類號】：R730.4;R737.25
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本文編號：2068049