本文選題：弱精子癥 + 精子DNA碎片��； 參考：《中南大學》2014年碩士論文

【摘要】：目的：1.研究弱精子癥患者精子DNA碎片率(DNA fragmentation index, DFI)與精液常規(guī)參數(shù)、受精結(jié)局、胚胎發(fā)育及精子非整倍體率的相關(guān)關(guān)系,以評價弱精子癥患者中精子DFI的臨床預測價值；2.比較上游、密度梯度離心、密度梯度離心聯(lián)合上游三種常規(guī)精液處理方法對精子DFI的影響,對臨床精液處理方法的選擇起到指導作用。 方法：1.收集82例弱精子癥患者的新鮮精液標本,每份標本進行精液常規(guī)參數(shù)檢測后均分為三份,分別進行①DFI檢測(吖啶橙染色法)；②卵胞漿內(nèi)單精子注射,并記錄受精及胚胎發(fā)育情況；③精子熒光原位雜交檢測(26例)非整倍體(18/21/X/Y)。根據(jù)DFI指數(shù)分組：A組(DFI≤15%,n=18)、B組(15%DFI30%, n=38)、C組(DFI≥30%,n=26)。2.收集38例精子完整性差(DFI≥30%)患者的新鮮精液標本,每份標本均分為三份,分別進行上游(CSW)、密度梯度離心(DGC)、密度梯度離心聯(lián)合上游(DGC+CSW)處理,檢測三種方法處理前后精液常規(guī)參數(shù)和DFI指數(shù)。 結(jié)果：1.C組的前向運動精子百分率(PR,%)及精子總活力(PR+NP,%)均顯著低于A組和B組(P0.05)；C組的優(yōu)胚率及精子非整倍體率均顯著低于A組(P0.05)；精子DFI與PR、PR+NP負相關(guān)(P0.05),與精子非整倍體率正相關(guān)(P0.05)。2.CSW、DGC、DGC+CSW三種方法處理后精子PR、PR+NP均升高(P0.05),濃度均降低(P0.05)。CSW、DGC+CSW處理后DFI均降低(P0.05),但DGC處理后DFI升高(P0.05)；三種方法處理結(jié)果兩兩比較時,CSW、DGC+CSW處理后PR、PR+NP均高于DGC(P0.05),但濃度均低于DGC(P0.05), DGC+CSW處理后DFI顯著低于CSW(P0.05)。 結(jié)論：1.弱精子癥患者精子DFI指數(shù)具有一定的臨床預測價值；2.與單純上游或密度梯度離心法相比,密度梯度離心聯(lián)合上游法能更有效篩選出活力好,DNA完整的精子。
[Abstract]:Purpose 1. To evaluate the clinical predictive value of sperm fragmentation fragment rate (DFI) in patients with asthenospermia, we studied the relationship between sperm fragmentation fragment rate and semen routine parameters, fertilization outcome, embryo development and sperm aneuploidy rate in order to evaluate the clinical predictive value of sperm fragmentation rate in patients with asthenospermia. Compared with the upstream, density gradient centrifugation combined with the upstream three conventional semen treatment methods on sperm DFI, the selection of clinical semen processing methods play a guiding role. Method 1: 1. Fresh semen samples from 82 patients with asthenospermia were collected. Each specimen was divided into three samples after routine semen parameters were measured. The sperm was injected into oocytes by 1DFI (acridine orange staining). The fertilization and embryo development were recorded by fluorescence in situ hybridization (26 cases) and aneuploidy (18 / 21% X / Y). According to DFI index, group A (DFI 鈮，

本文編號：2066656