本文選題：染料木素 + 尿毒癥��； 參考：《重慶醫(yī)學(xué)》2017年07期

【摘要】：目的 探討染料木素對(duì)尿毒癥大鼠細(xì)胞外基質(zhì)(ECM)沉積的影響及其機(jī)制。方法 5/6腎切除術(shù)建立尿毒癥模型鼠,將模型鼠分為染料木素組(G組)和對(duì)照組(C組),假手術(shù)組(S組)作為正常對(duì)照。檢測(cè)各組術(shù)前及術(shù)后第4、8周尿蛋白和生化等指標(biāo),第8周觀察腎組織病理變化情況,半定量法評(píng)估腎小球硬化指數(shù)(GSI)。免疫組化法檢測(cè)Ⅳ型膠原(ColⅣ)、纖維連接蛋白(FN)的沉積情況。應(yīng)用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)法、Western blot法分別檢測(cè)腎組織中轉(zhuǎn)化生長因子β1(TGF-β1)mRNA轉(zhuǎn)錄、蛋白質(zhì)表達(dá)情況。結(jié)果 術(shù)后第4周與C組比較,G組尿蛋白排泄量減少[(11.63±2.07)vs.(19.93±3.19)mg/d,P0.01],血清尿素氮、肌酐水平降低[(9.39±0.59)vs.(12.09±0.78)mmol/L,(65.11±3.79)vs.(77.63±3.20)μmol/L,P0.01]。至術(shù)后第8周,尿蛋白排泄量減少,血清尿素氮、肌酐水平降低更為顯著。腎小球內(nèi)ColⅣ和FN沉積減少[(17.30±1.96)%vs.(24.68±3.97)%,(18.26±2.31)%vs.(29.35±4.15)%,P0.01],腎小球硬化明顯減輕。TGF-β1mRNA、蛋白質(zhì)表達(dá)減弱(P0.05)。結(jié)論 染料木素可以減少尿毒癥大鼠細(xì)胞ECM的蓄積,對(duì)腎臟具有保護(hù)作用。
[Abstract]:Objective to investigate the effect and mechanism of genistein on ECM deposition in uremic rats. Methods the uremic model rats were established by 5 / 6 nephrectomy. The rats were divided into genistein group (group G), control group (group C) and sham operation group (group S) as normal control. Urine protein and biochemical indexes were measured before and after operation, renal pathological changes were observed at the 8th week, and glomerulosclerosis index (GSI) was evaluated by semi-quantitative method. The deposition of Col 鈪，

本文編號(hào)：2044690