本文選題：FcγRIB　切入點(diǎn)：基因多態(tài)性　出處：《吉林大學(xué)》2014年碩士論文

【摘要】：介紹：膜性腎病是成人原發(fā)性腎病綜合征最常見的病因。其病理顯示沿腎小球毛細(xì)血管壁四周可發(fā)現(xiàn)免疫沉積物包含免疫球蛋白IgG和補(bǔ)體，呈顆粒狀分布。Boruchov等的研究表明，F(xiàn)cγ受體IIB有助于維持B細(xì)胞的耐受性，同時(shí)提供了第一個(gè)論據(jù)證明它可能誘導(dǎo)T細(xì)胞發(fā)揮作用而限制樹突狀細(xì)胞的成熟。因此，這些結(jié)果表明Fcγ受體IIB在由免疫復(fù)合物介導(dǎo)的疾病發(fā)病機(jī)制中有重要作用。Fcγ受體IIBI232T是一個(gè)功能受損的變異。它已被證實(shí)與自身免疫性疾病的易感性相關(guān)，如系統(tǒng)性紅斑狼瘡。但到現(xiàn)在為止，還沒有太多的研究來探討其與免疫復(fù)合物介導(dǎo)疾病的相關(guān)性，如膜性腎病等。所以在本研究中，我們打算探討Fcγ受體IIBI232T基因多態(tài)性與膜性腎病易感性的關(guān)聯(lián)。 材料與方法：80名健康對(duì)照組血樣和26名膜性腎病患者組血樣分別來自于吉林大學(xué)中日聯(lián)誼體檢中心和腎內(nèi)科，標(biāo)本采集時(shí)間為2012年至2013年。膜性腎病的病理診斷由吉林大學(xué)基礎(chǔ)醫(yī)學(xué)院病理科診斷，組織標(biāo)本儲(chǔ)存于吉林大學(xué)中日聯(lián)誼醫(yī)院組織標(biāo)本庫。通過實(shí)時(shí)定量熒光PCR對(duì)Fcγ受體IIBI232T進(jìn)行基因分型。健康組和膜性腎病患者組的I232T基因頻率使用標(biāo)準(zhǔn)統(tǒng)計(jì)方法進(jìn)行比較。 結(jié)果：健康對(duì)照組中232I/I型為43例，占53.25%；232I/T型為37例，占46.25%；232T/T型為0例；其中I的基因頻率為76.88%，T的基因頻率為23.13%。膜性腎病患者組中232I/I型為8例，占30.77%；232I/T型為18例，占69.23%；232T/T型為0例；其中I的基因頻率為65.38%，T的基因頻率為34.62%。 結(jié)論：比較FcγRIIBI232T基因多態(tài)性在膜性腎病病例組與正常對(duì)照組之間的分布，按照基因型方法計(jì)算時(shí)發(fā)現(xiàn)兩者存在顯著差別，表示FcγRIIBI232T是膜性腎病的危險(xiǎn)因素（X2=4.151,P=0.0420.05,OR=2.591）。但按照等位基因呈現(xiàn)度和等位基因頻率方法計(jì)算時(shí)，，兩者則無統(tǒng)計(jì)學(xué)意義（P0.05）。
[Abstract]:This paper introduces that membranous nephropathy is the most common etiology of adult primary nephrotic syndrome. Studies showing granular distribution. Boruchov et al. suggest that FC 緯 receptor IIB contributes to the maintenance of B cell tolerance and provides the first evidence that it may induce T cells to function and limit the maturation of dendritic cells. These results suggest that FC 緯 receptor IIB plays an important role in the pathogenesis of disease mediated by immune complex. FC 緯 receptor IIBI232T is a functionally impaired variant, which has been confirmed to be associated with susceptibility to autoimmune diseases. For example, systemic lupus erythematosus. But so far, there has not been much research on its association with immune complex mediated diseases, such as membranous nephropathy. We intend to explore the association between FC 緯 receptor IIBI232T gene polymorphism and susceptibility to membranous nephropathy. Materials and methods Blood samples of 80 healthy controls and 26 patients with membranous nephropathy were collected from the Sino-Japanese Medical examination Center and the Department of Renal Medicine, Jilin University, respectively. Specimens were collected from 2012 to 2013. The pathological diagnosis of membranous nephropathy was diagnosed by the Department of Pathology, School of basic Medicine, Jilin University. Tissue samples were stored in the tissue library of Sino-Japanese Friendship Hospital of Jilin University. FC 緯 receptor IIBI232T was genotyped by real-time quantitative PCR. The frequency of I232T gene was compared between healthy group and patients with membranous nephropathy. Results: there were 43 cases of 232I/I type, 37 cases of 232I / T type and 0 case of 232T / T type of 46.25 cases in the healthy control group, in which the gene frequency of I was 76.88 T / T 23.13. In the membranous nephropathy group, there were 8 cases of 232I/I type, 18 cases of 30.77232I- / T type and 0 case of 69.23232T / T type. The gene frequency of I was 65.38 and the gene frequency of T was 34.62. Conclusion: the distribution of FC 緯 RIIBI232T gene polymorphism in patients with membranous nephropathy was compared with that in normal controls. The results showed that FC 緯 RIIBI232T was a risk factor for membranous nephropathy.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R692.3

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