發(fā)布時(shí)間：2018-03-31 14:13

  本文選題：膀胱尿路上皮癌　切入點(diǎn)：胸苷激酶1(TK1)　出處：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：探討胸苷激酶1(thymidine kinase1，TK1)和細(xì)胞核增殖相關(guān)抗原67(nuclear-associated antigen67，Ki-67)在膀胱尿路上皮癌中的表達(dá)及其相關(guān)性，了解TK1可否作為膀胱癌的預(yù)后因素。 方法：收集廣西醫(yī)科大學(xué)附屬腫瘤醫(yī)院2010-2013年間病理證實(shí)為膀胱尿路上皮癌的病理標(biāo)本47例和正常膀胱粘膜標(biāo)本10例。應(yīng)用免疫組化SP法檢測TK1、Ki-67在以上標(biāo)本中的表達(dá)，進(jìn)而研究TK1和Ki-67的表達(dá)與膀胱尿路上皮癌臨床病理參數(shù)的關(guān)系及其相關(guān)性。應(yīng)用SPSS16.0進(jìn)行統(tǒng)計(jì)處理，各組間計(jì)數(shù)資料的比較采用卡方檢驗(yàn)，相關(guān)分析采用Spearman分析，以α=0.05為顯著性檢驗(yàn)水準(zhǔn)，P0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果：1. TK1和Ki-67在膀胱尿路上皮癌組織中的表達(dá)明顯高于正常膀胱黏膜組織，差異有統(tǒng)計(jì)學(xué)意義（P0.05）。 2.膀胱尿路上皮癌組織中TK1的陽性表達(dá)與腫瘤分期有關(guān)(P0.05)；Ki-67的陽性表達(dá)與腫瘤分期和病理分級均有關(guān)（P0.05）。 3.TK1和Ki-67在膀胱尿路上皮癌中的表達(dá)成正相關(guān)(r=0.371,P=0.001)。 結(jié)論1.過表達(dá)的TK1和Ki-67可能與膀胱黏膜過度增殖和惡性轉(zhuǎn)化有關(guān)。 2．在病理分化越差的膀胱尿路上皮癌組織中，，其Ki-67表達(dá)水平越高。 3. TK1和Ki-67在與膀胱尿路上皮癌腫瘤分期的關(guān)系方面表現(xiàn)出相關(guān)性：在肌層浸潤性膀胱尿路上皮癌中的表達(dá)明顯高于非肌層浸潤性膀胱尿路上皮癌。 4.本研究用免疫組化方法顯示TK1在膀胱尿路上皮癌的表達(dá)與膀胱癌的分期密切相關(guān)，但在膀胱癌的不同病理分級中表達(dá)差異性無顯著性統(tǒng)計(jì)學(xué)意義。TK1是否能作為膀胱癌的預(yù)后評估因素，尚需擴(kuò)大樣本及對病人生存期等方面的研究。
[Abstract]:Objective: to investigate the expression of thymidine kinase (1(thymidine kinase1) and nuclear proliferation-associated antigen (67(nuclear-associated antigen67 Ki-67) in bladder urothelial carcinoma, and to investigate whether TK1 can be used as a prognostic factor of bladder cancer.Methods: 47 cases of bladder urothelial carcinoma and 10 cases of normal bladder mucosa were collected from Cancer Hospital affiliated to Guangxi Medical University from 2010 to 2013.Immunohistochemical SP method was used to detect the expression of TK1 Ki-67 in the above specimens, and to study the relationship between the expression of TK1 and Ki-67 and the clinicopathological parameters of bladder urothelial carcinoma.The statistics were processed by SPSS16.0, the data of counting were compared by chi-square test, the correlation analysis by Spearman analysis, using 偽 -0.05as significant test level (P0.05) as the difference was statistically significant.The result is 1: 1.The expression of TK1 and Ki-67 in bladder urothelial carcinoma was significantly higher than that in normal bladder mucosa (P 0.05).2.The positive expression of TK1 in bladder urothelial carcinoma was related to the tumor stage. The positive expression of Ki-67 was correlated with the tumor stage and pathological grade.The expression of 3.TK1 and Ki-67 in bladder urothelial carcinoma was positively correlated with that of 0.371P.Conclusion 1.Overexpression of TK1 and Ki-67 may be associated with excessive proliferation and malignant transformation of bladder mucosa.2.The lower the pathological differentiation, the higher the expression of Ki-67 in bladder urothelial carcinoma.3.The expression of TK1 and Ki-67 in bladder urothelial carcinoma was significantly higher than that in non-myometrial invasive bladder urothelial carcinoma.4.The expression of TK1 in bladder urothelial carcinoma was closely related to the stage of bladder cancer.However, there is no significant difference in the expression of TK1 in different pathological grades of bladder cancer. Whether TK1 can be used as a prognostic factor of bladder cancer, it is necessary to expand the sample and study the survival time of the patients.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.14

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