本文選題：Maresin　切入點(diǎn)：糖尿病腎病　出處：《山東醫(yī)藥》2017年46期

【摘要】：目的觀察Maresin1對(duì)高糖刺激小鼠腎小球系膜細(xì)胞炎癥反應(yīng)的影響及其對(duì)核苷酸結(jié)合寡聚化結(jié)構(gòu)域樣受體蛋白3(NLRP3)炎癥小體激活分泌炎癥因子的抑制作用。方法培養(yǎng)小鼠腎小球系膜細(xì)胞株,NC組培養(yǎng)于低糖DMEM培養(yǎng)基中;OP組在低糖DMEM培養(yǎng)基中加入甘露醇;HG組培養(yǎng)于高糖DMEM培養(yǎng)基中;M1+HG組、M2+HG組、M3+HG組分別用1、10、100 nmol/L的Maresin1預(yù)處理后在高糖培養(yǎng)基中培養(yǎng);M3+NC組用100nmol/L的Maresin 1預(yù)處理30 min后在低糖DMEM培養(yǎng)基中培養(yǎng);另設(shè)N-乙酰半胱氨酸(NAC)+HG組作為活性氧抑制作用的陽(yáng)性對(duì)照。用全光譜分光光度計(jì)和熒光顯微鏡觀察活性氧(ROS)表達(dá)情況;采用RT-PCR法檢測(cè)NLRP3、Caspase-1、IL-1βmRNA表達(dá);采用Western blotting法檢測(cè)NLRP3、pro-Caspase-1、Caspase-1、pro-IL-1β、IL-1β蛋白表達(dá)。結(jié)果與NC組相比,HG組細(xì)胞內(nèi)ROS水平與NLRP3、Caspase-1、IL-1β蛋白及mRNA表達(dá)均增加,pro-Caspase-1、pro-IL-1β蛋白表達(dá)降低(P均0.05);與HG組相比,M1+HG組、M2+HG組、M3+HG組ROS水平與NLRP3、Caspase-1、IL-1β蛋白及mRNA表達(dá)均降低,pro-Caspase-1、pro-IL-1β蛋白表達(dá)均增加(P均0.05),以M3+HG組作用最明顯。與HG組相比,M3+HG組NLRP3、Caspase-1、IL-1β蛋白及mRNA表達(dá)均降低,proCaspase-1、pro-IL-1β表達(dá)均增加(P均0.05)。結(jié)論 Maresin1可抑制高糖刺激導(dǎo)致的小鼠腎小球系膜細(xì)胞氧化應(yīng)激反應(yīng)及炎癥反應(yīng),其機(jī)制可能與降低ROS水平、抑制NLRP3炎癥小體及相關(guān)炎癥因子的表達(dá)有關(guān)。
[Abstract]:Objective To observe the effect of Maresin1 on glucose stimulated mouse mesangial cells and inflammatory reaction of nucleotide binding oligomerization domain protein like receptor 3 (NLRP3) inflammasome activation inhibition of inflammatory cytokine secretion. Methods cultured mouse mesangial cells, NC group cultured in low glucose DMEM medium in OP group; low sugar DMEM medium with mannitol medium; HG group cultured in high glucose DMEM medium; M1+HG group, M2+HG group, M3+HG group were pretreated with Maresin1 1,10100 nmol/L in high glucose medium; M3+NC group with 100nmol/L Maresin 1 pretreatment after 30 min in low sugar DMEM medium; the other set of N- acetylcysteine (NAC) positive control group +HG as inhibition of ROS. With full spectrum spectrophotometer and fluorescence microscope observation of reactive oxygen species (ROS) expression was detected by RT-PCR; NLRP3, Caspase-1, IL-1 beta mR The expression of NA; using Western blotting method to detect NLRP3, pro-Caspase-1, Caspase-1, pro-IL-1 beta, the expression of IL-1 beta protein. Results compared with NC group, ROS level and NLRP3, HG group, Caspase-1, expression of IL-1 protein and mRNA were increased, pro-Caspase-1 decreased the expression of pro-IL-1 beta protein (P 0.05); compared with HG group, M1+HG group, M2+HG group, M3+HG group, ROS level and NLRP3, Caspase-1, expression of IL-1 protein and mRNA were decreased, pro-Caspase-1 was increased, the expression of pro-IL-1 beta protein (P 0.05), the effect of M3+HG was most obvious. Compared with the HG group, M3+HG group, NLRP3, Caspase-1, expression of IL-1 protein and mRNA decreased proCaspase-1, pro-IL-1 expression were increased (P 0.05). Mouse mesangial cells in response to oxidative stress and inflammatory reaction. Conclusion: Maresin1 can inhibit high glucose induced, and its mechanism may reduce the level of ROS, inhibition of NLRP3 inflammasome and related inflammatory factors The expression is related.

【作者單位】： 核工業(yè)四一六醫(yī)院;西南醫(yī)科大學(xué)附屬醫(yī)院;
【分類(lèi)號(hào)】：R587.2;R692.9

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