本文選題：MiR-a　切入點(diǎn)：慢性腎衰竭　出處：《第三軍醫(yī)大學(xué)學(xué)報》2015年05期 　論文類型：期刊論文

【摘要】：目的分析microRNA-30a(miR-30a)在慢性腎衰竭患者透析前后血清中的表達(dá)差異,評價其在評估慢性腎衰竭血液透析效果中的作用。方法選取2012年10月至2013年12月本科收治的慢性腎衰竭患者40例,分別在患者透析前后采血并獲得血清樣本,采用mirV ana PARIS Kit提取血清樣本中總RNA,利用qRT-PCR法檢測miR-30a的表達(dá),臨床生化檢測患者透析前后血清中肌酐(Cr)、尿素氮(Ur)及β2微球蛋白(β2-MG)的含量,表達(dá)值行曼-惠特尼U檢驗(yàn),工作特征曲線(ROC curve)評估m(xù)iR-30a在慢性腎衰竭患者透析效果中的價值。結(jié)果 miR-30a、Ur和β2-MG在慢性腎衰竭患者透析后的表達(dá)顯著低于透析前(P0.05,P0.01),ROC curve顯示在患者透析后miR-30a的表達(dá)低于透析前0.25倍以上時患者可獲得較好的透析效果。結(jié)論 miR-30a可作為一種潛在的評估慢性腎衰竭血液透析效果的生物標(biāo)志物。
[Abstract]:Objective to analyze the difference of microRNA-30a miR-30a in serum of patients with chronic renal failure (CRF) before and after dialysis, and to evaluate the role of microRNA-30aAmiR-30a in evaluating the hemodialysis effect of chronic renal failure (CRF). Methods 40 patients with CRF were selected from October 2012 to December 2013. Serum samples were collected before and after dialysis. The total RNAs in serum samples were extracted by mirV ana PARIS Kit, and the expression of miR-30a was detected by qRT-PCR method. Before and after dialysis, the serum creatinine (Cr), urea nitrogen (Url) and 尾 _ 2-microglobulin (尾 _ 2-MG) in the serum of the patients were detected and the expression values were measured by Man-Whitney U test. Results the expression of miR-30aUr and 尾 2-MG in patients with chronic renal failure after dialysis was significantly lower than that in patients with chronic renal failure after dialysis. Conclusion miR-30a can be used as a potential biomarker to evaluate the hemodialysis effect of chronic renal failure.
【作者單位】： 第三軍醫(yī)大學(xué)新橋醫(yī)院腎內(nèi)科;
【基金】：國家自然科學(xué)基金(30800528)~~
【分類號】：R692.5

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 袁泉;柳滿然;曾宗躍;周旭春;;microRNA與胃癌耐藥的關(guān)系[J];國際檢驗(yàn)醫(yī)學(xué)雜志;2013年17期

2 Patrick-Denis St-Coeur;Mohamed Touaibia;Miroslava Cuperlovic-Culf;Pier Jr Morin;;Leveraging Metabolomics to Assess the Next Generation of Temozolomide-based Therapeutic Approaches for Glioblastomas[J];Genomics, Proteomics & Bioinformatics;2013年04期

3 楊先濤;張禎禎;詹學(xué);朱朝敏;;肺結(jié)核患兒外周血單個核細(xì)胞中microRNA表達(dá)的初步研究[J];第三軍醫(yī)大學(xué)學(xué)報;2013年19期

4 Xiao-Hui Zheng;Cui Cui;Xin-Xi Zhou;Yi-Xin Zeng;Wei-Hua Jia;;Centrifugation: an important pre-analytic procedure that influences plasma microRNA quantification during blood processing[J];Chinese Journal of Cancer;2013年12期

5 Wenwen Jia;Wen Chen;Jiuhong Kang;;The Functions of MicroRNAs and Long Non-coding RNAs in Embryonic and Induced Pluripotent Stem Cells[J];Genomics,Proteomics & Bioinformatics;2013年05期

6 林松;范磊;邵增務(wù);;微RNA調(diào)控自噬研究進(jìn)展[J];國際骨科學(xué)雜志;2013年05期

7 張世芳;魏彩虹;陸健;張小寧;周鑫磊;張淑珍;王光凱;曹家雪;趙福平;張莉;杜立新;;深度測序鑒定綿羊microRNA轉(zhuǎn)錄組[J];中國畜牧獸醫(yī);2013年09期

8 葉辛;張蕾;石磊;谷明莉;張薇薇;張建榮;秦琴;錢寶華;鄧安梅;;免疫性血小板減少性紫癜患者單個核細(xì)胞中microRNA-30a表達(dá)增高及其意義[J];國際檢驗(yàn)醫(yī)學(xué)雜志;2013年24期

9 Hong Zheng;Jia-Yu Liu;Feng-Ju Song;Ke-Xin Chen;;Advances in circulating microRNAs as diagnostic and prognostic markers for ovarian cancer[J];Cancer Biology & Medicine;2013年03期

10 徐t，

本文編號：1621687