本文選題：富血小板纖維蛋白 + 異種凍干骨��； 參考：《河北醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：通過對兔雙側(cè)下頜無牙區(qū)牙槽骨行骨極限缺損造模后分別植入富血小板纖維蛋白（Platelet-rich fibrin, PRF）異種凍干骨復(fù)合物及異種凍干骨,并同期植入鈦螺紋釘,觀察并檢測缺損區(qū)新骨形成及釘周骨整合情況,探討PRF和異種凍干骨在骨修復(fù)及骨整合中的作用機(jī)制，為臨床應(yīng)用提供實驗參考。 方法： 1分組：健康成年新西蘭大白兔12只，雌雄不限，隨機(jī)分為3組，每組4只，分別于術(shù)后4812周處死。所有實驗動物采取自身對照方法，右側(cè)下頜骨極限缺損區(qū)為實驗組，植入PRF"異種凍干骨復(fù)合物；左側(cè)下頜骨極限缺損區(qū)為對照組，，植入異種凍干骨。 2異種凍干骨獲取及制備：取自人骨組織，經(jīng)骨髓清洗深凍凍干和射線輻照等過程制備。 3PRF的制備：于兔耳中央動脈處快速抽取5ml血置于未添加抗凝劑的采血管中，迅速置于已配平的離心機(jī)中，以3000r／min的速度離心10min,停止后取出離心管靜置，可見管內(nèi)血液分為3層：最上層為貧血小板血漿層，中間層為纖維蛋白凝膠層，最底層為紅細(xì)胞碎片層。棄上清液，分離中間的纖維蛋白凝膠，即為PRF。5ml兔動脈血可制備約7.5×4.0×0.4mm的PRF。 4造模及干預(yù)：在每只實驗動物的雙側(cè)下頜骨門齒遠(yuǎn)中無牙區(qū)牙槽骨各造一約5×4×3mm的缺損，缺損后緣位于頦孔前約4mm處，其下緣與頦孔平齊。分別自缺損區(qū)近遠(yuǎn)中壁遠(yuǎn)離缺損區(qū)方向以鈦螺紋釘配套鉆針制備一約直徑1.5mm，深3.5mm的種植窩，將鈦螺紋釘擰入種植窩，右側(cè)為實驗組，缺損區(qū)充填PRF"異種凍干骨復(fù)合物；左側(cè)為對照組，缺損區(qū)僅植入異種凍干骨，逐層縫合，術(shù)后正常飲食。 5觀察結(jié)果：分別于術(shù)后4812周以空氣栓塞法處死實驗動物，沿兔口角做切口，分離出下頜骨，去凈其上附麗組織，大體肉眼觀察骨極限缺損區(qū)愈合修復(fù)情況；扭矩實驗測試鈦螺紋釘-骨界面的結(jié)合強(qiáng)度，收集數(shù)據(jù)進(jìn)行統(tǒng)計學(xué)分析；組織學(xué)觀察，各標(biāo)本切片行HE染色后，采集圖像，通過多功能真彩色細(xì)胞圖像分析管理系統(tǒng)分析計算新骨形成面積占視野區(qū)面積的百分比，進(jìn)行新生骨定量分析。 結(jié)果： 1標(biāo)本大體肉眼觀察結(jié)果：實驗組及對照組中所植入的鈦螺紋釘均無松動脫落，所造骨組織缺損區(qū)均逐漸被骨組織覆蓋，表面有不同程度凹陷，實驗組顯示表面形態(tài)恢復(fù)更為理想。 2術(shù)后4812周同一時間點扭矩測試實驗組種植體-骨結(jié)合強(qiáng)度高于對照組，結(jié)果有顯著性差異，有統(tǒng)計學(xué)意義（P＜0.05）。 3組織學(xué)觀察術(shù)后4812周同一時間點實驗組新生骨面積與對照組比較，有統(tǒng)計學(xué)意義（P＜0.05）。 結(jié)論： 1在下頜牙槽骨極限缺損情況下，PRF"異種凍干骨復(fù)合物及異種凍干骨均有助于骨缺損修復(fù)及鈦螺紋釘周骨整合。 2PRF"異種凍干骨復(fù)合物有更好的骨傳導(dǎo)和骨誘導(dǎo)能力，可以加快骨整合，促進(jìn)修復(fù)進(jìn)程，較單獨使用異種凍干骨效果好。
[Abstract]:Objective: to model the alveolar bone defect in bilateral edentulous region of rabbits and to implant platelet-rich fibrin (PRF) xenogeneic freeze-dried bone complex and xenogeneic freeze-dried bone respectively, and to implant titanium thread nail simultaneously. To investigate the mechanism of PRF and xenogeneic freeze-dried bone in bone repair and bone integration, to observe and detect the formation of new bone and bone integration around nail in defect area, and to provide experimental reference for clinical application. Methods: 1 A total of 12 healthy adult New Zealand white rabbits were randomly divided into 3 groups with 4 rabbits in each group. The rabbits were killed at 812 weeks after operation. All experimental animals were treated with self-control method. The right mandibular limit defect region was used as experimental group and PRF "xenogeneic freeze-dried bone complex" was implanted into the experimental group, while the left mandibular limiting defect area was used as the control group. Xenogeneic freeze-dried bone was implanted. 2 xenogeneic freeze-dried bone was obtained and prepared: from human bone tissue, cleaned by bone marrow? Deep freeze? Rapid extraction of 5ml blood from the central artery of rabbit ear and placement of 5ml blood in the blood vessel without anticoagulant, and in a leveled centrifuge. After centrifugation at 3000r/min speed for 10 mins, the centrifuge tube was taken out to rest, and the blood in the tube was divided into three layers: the highest layer was the platelet-poor plasma layer, the middle layer was the fibrin gel layer, and the bottom layer was the red blood cell fragment layer. The fibrin gel was separated from the supernatant, that is, PRF.5ml rabbit arterial blood could be used to prepare a PRF.4 model of 7.5 脳 4.0 脳 0.4mm and its intervention: a defect of about 5 脳 4 脳 3mm was made in the alveolar bone of each bilateral mandibular incisor in the distal part of the mandibular incisor. The posterior edge of the defect was located about 4mm in front of mental foramen, and the lower margin was level with mental foramen. Respectively from the defect area near? The distal wall is far away from the defect area and the titanium thread screw matching needle is used to prepare the implant nest, which is 1.5mm in diameter and deep in 3.5mm. The titanium thread nail is screwed into the implant nest, the right side is the experimental group, the defect area is filled with the 3.5mm "dissimilar freeze-dried bone complex", the left side is the control group, and the left side is the control group. Only allogeneic freeze-dried bone was implanted in the defect area and sutured layer by layer. 5 observation results: the experimental animals were killed by air embolization 12 weeks after operation, and the mandible was separated from the mandible by incision along the rabbit mouth angle. After removing the attached tissue, the healing and repairing of the ultimate defect area was observed with the naked eye; the binding strength of the interface between the titanium thread nail and the bone was measured by torque experiment, and the data was collected for statistical analysis; the histological observation was performed on the sections of each specimen after HE staining. The image was collected and the percentage of the area of new bone formation to the area of visual field was calculated by the analysis and management system of multifunctional true color cell image, and the quantitative analysis of new bone was carried out. Results: 1 the results of gross naked eye observation: the titanium screw implanted in the experimental group and the control group had no loosening and falling off, the defect area of the bone tissue was gradually covered by bone tissue, and the surface of the bone tissue was sunken in varying degrees. The surface morphology of the experimental group was better than that of the control group. (2) the strength of implant osseous bond in the experimental group was higher than that in the control group at the same time point at 12 weeks after operation, and there was significant difference in the results. There was significant difference between the experimental group and the control group (P < 0.05). 3 there was significant difference in bone area between the experimental group and the control group at the same time point at 12 weeks after operation (P < 0.05). Conclusion: 1 under the condition of limiting defect of mandibular alveolar bone, PRF "dissimilar freeze-dried bone complex and xenogeneic freeze-dried bone are helpful to repair bone defect and integrate bone around titanium screw. Better bone conduction and bone induction, It can speed up bone integration and promote the process of repair, which is better than using xenogeneic freeze-dried bone alone.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊穎;仲維劍;馬國武;;富血小板纖維蛋白用于骨組織再生研究進(jìn)展[J];中國實用口腔科雜志;2012年12期

2 廖鳳春;周諾;黃旋平;;凍干骨在骨缺損修復(fù)中的研究進(jìn)展[J];中國矯形外科雜志;2012年22期

3 鄂玲玲;王東勝;師占平;吳霞;呂燕;王家柱;劉洪臣;;一種新型的兔牙槽骨缺損模型的建立[J];中華老年口腔醫(yī)學(xué)雜志;2011年03期

4 毛晶晶;何家才;鄭先雨;周華杰;;富血小板纖維蛋白在犬上頜竇底提升中促骨缺損修復(fù)的作用[J];安徽醫(yī)科大學(xué)學(xué)報;2010年02期

5 宋珂;曹穎光;;多元化骨組織工程修復(fù)頜骨缺損的研究進(jìn)展[J];臨床口腔醫(yī)學(xué)雜志;2010年02期

6 康軍;沙月琴;歐陽翔英;;脫礦凍干骨粉與富血小板血漿聯(lián)合治療牙周骨下袋[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2010年01期

7 周延民;李琦;儲順禮;李春艷;王宇;;即刻種植間隙充填富血小板纖維蛋白修復(fù)骨缺損2例[J];中國實用口腔科雜志;2009年08期

8 周延民;;Choukroun's PRF在種植臨床的應(yīng)用[J];中國口腔種植學(xué)雜志;2009年02期

9 許亦權(quán);胡敏;孫立群;王陽;肖紅喜;李巖峰;姚昊;;同種異體下頜骨移植修復(fù)犬顳頜關(guān)節(jié)、下頜骨缺損的免疫學(xué)觀察[J];中國組織工程研究與臨床康復(fù);2008年53期

10 陳鐵樓;劉國勤;吳織芬;周以鈞;;異種骨移植對牙周骨缺損的作用及研究進(jìn)展[J];牙體牙髓牙周病學(xué)雜志;2007年08期

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