本文選題：血管性癡呆 + Morris水迷宮��； 參考：《延邊大學(xué)》2013年碩士論文

【摘要】：目的：觀察朝醫(yī)三神散(sanshensan,SSS)對(duì)血管性癡呆(Vascular Dementia, VD)模型大鼠血清中超氧化物歧化酶(supermxidedismutase, SOD)、丙二醛(malonaldchydc, MDA)、谷胱甘肽過(guò)氧化物酶(glulathione pcroxidase, GSH-PX)水平的影響,探討其治療血管性癡呆的療效機(jī)制。方法：采用wistar系雄性大鼠42只,隨機(jī)分為假手術(shù)組、手術(shù)組,手術(shù)組采用改良2-血管阻斷法(2-VO)+硝普鈉降壓法制作VD模型,假手術(shù)組動(dòng)物頸總動(dòng)脈切開(kāi)不夾閉。將VD模型大鼠隨機(jī)分為假手術(shù)組、模型組、三神散大劑量組、三神散中劑量組、三神散小劑量組、喜得鎮(zhèn)(Hyderginc)組,灌藥各組給予不同的藥物灌胃,假手術(shù)組及模型組給予等劑量的蒸餾水灌胃,連續(xù)灌胃28天,處死制取標(biāo)本。觀察VD模型大鼠學(xué)習(xí)記憶能力及血清中SOD、MDA、GSH-PX水平的變化,探討VD的發(fā)病機(jī)制和三神散對(duì)VD模型大鼠缺血腦組織的保護(hù)作用。結(jié)果：1.與假手術(shù)組比較,模型組學(xué)習(xí)記憶能力明顯降低(P0.05)。2.與模型組對(duì)照,各治療組學(xué)習(xí)記憶能力明顯改善(P0.05)。3.與假手術(shù)組比較,模型組血清SOD、GSH-PX含量明顯降低,MDA含量明顯升高(P0.05)。4.與模型組對(duì)照,各治療組血清SOD、GSH-PX含量均明顯升高(P＜0.01), MDA含量下降(P0.01)。5.與喜得鎮(zhèn)組比較,三神散小劑量組SOD, GSH-PX, GSH含量明顯降低(P＜0.05), MDA含量明顯升高(P0.05),而三神散大、中劑量組SOD、GSH-PX、MDA含量差異不顯著(P0.05)。結(jié)論：通過(guò)Morris水迷宮實(shí)驗(yàn),觀察和比較三神散對(duì)VD模型大鼠學(xué)習(xí)記憶能力的影響,說(shuō)明三神散能改善VD大鼠學(xué)習(xí)記憶的能力。三神散可明顯升高血管性癡呆模型大鼠血清中SOD、GSH-PX的活性,降低脂質(zhì)氧化產(chǎn)物MDA的含量。
[Abstract]:Objective: to observe the effect of Sanshensan San (SSSs) on the levels of superoxide dismutase (SOD), superoxide dismutase (SOD), malondialdehyde (malonaldchydcc), glutathione peroxidase (Glutathione peroxidase) pcroxidase (GSH-PX) in the serum of vascular dementia rats, and to explore the mechanism of their therapeutic effect in the treatment of vascular dementia. Methods: the serum levels of superoxide dismutase (SOD), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (Glutathione peroxidase) (GSH-PX) in the serum of vascular dementia rats were observed.Methods: Forty-two male wistar rats were randomly divided into two groups: sham operation group, operation group, and modified 2-VOO sodium nitroprusside sodium hypotensive method to make VD model. In sham operation group, the common carotid artery was not clipped.VD rats were randomly divided into sham operation group, model group, large dose group, middle dose group, low dose group and Hyderginc group.Sham operation group and model group were given the same dose of distilled water.To observe the changes of learning and memory ability and the level of GSH-PX in serum of VD model rats, to explore the pathogenesis of VD and the protective effect of San Shen San on ischemic brain tissue of VD model rats.The result is 1: 1.Compared with sham operation group, the learning and memory ability of model group was significantly lower than that of sham operation group.Compared with the model group, the ability of learning and memory in each treatment group was significantly improved (P 0.05. 3).Compared with the sham-operated group, the serum level of GSH-PX in the model group decreased significantly and the MDA content increased significantly.Compared with the model group, the serum level of GSH-PX in each treatment group was significantly higher than that in the control group (P < 0.01), and the content of MDA was decreased (P < 0.01).Compared with Xidazen group, the content of SOD, GSH-PXX and GSH in the small dose group of Sanshen Powder decreased significantly (P < 0.05), and the content of MDA increased significantly (P < 0.05), while the content of MDA in the medium dose group was not significantly different from that in the medium dose group (P 0.05).Conclusion: the effects of Sanshen Powder on the learning and memory ability of VD rats were observed and compared by Morris water maze experiment, which indicated that San Shen San could improve the learning and memory ability of VD rats.Sanshen powder could increase the activity of SOD GSH-PX and decrease the content of lipid oxidation product MDA in the serum of vascular dementia rats.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R749.13
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本文編號(hào)：1757316