本文選題：骨髓間充質(zhì)干細(xì)胞 + Notch信號(hào)通路��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年05期

【摘要】：為探討γ-分泌酶抑制劑(DAPT)阻斷Notch信號(hào)通路對(duì)BMSCs分化肺泡上皮細(xì)胞過程中的影響。本研究采用BMSCs與MLE-12非接觸共培養(yǎng),并在共培養(yǎng)中加入DAPT,實(shí)驗(yàn)分3組:空白對(duì)照組、共培養(yǎng)組、DAPT共培養(yǎng)組。共培養(yǎng)10 d后用光鏡觀察BMSCs的形態(tài)結(jié)構(gòu)變化,Western blotting和RT-qPCR檢測Notch信號(hào)通路相關(guān)基因Notch1,Ⅱ型肺泡上皮細(xì)胞特異性表面活性蛋白(surfactant protein C,SPC)、Ⅰ型肺泡上皮細(xì)胞標(biāo)志水通道蛋白(aquaporin 5,AQP5)的表達(dá)。結(jié)果表明共培養(yǎng)10 d后的BMSCs變?yōu)樗其伮肥瘶由掀ぜ?xì)胞形態(tài);和空白對(duì)照組相比,共培養(yǎng)組、DAPT共培養(yǎng)組中Notch1、SPC、AQP5蛋白及mRNA表達(dá)升高(p0.05);與共培養(yǎng)組相比,DAPT共培養(yǎng)組中Notch1、SPC、AQP5蛋白及mRNA的表達(dá)減少(p0.05)。因此推測BMSCs在MLE-12誘導(dǎo)下可以定向分化為肺泡上皮細(xì)胞,且分化的過程中Notch信號(hào)通路被激活,DAPT阻斷Notch信號(hào)通路能抑制BMSCs分化為肺泡上皮細(xì)胞。
[Abstract]:To investigate the effect of 緯 -secretase inhibitor (DAPT) blocking Notch signaling pathway on the differentiation of BMSCs into alveolar epithelial cells. In this study, BMSCs were co-cultured with MLE-12 and DAPT was added into co-culture. The experiment was divided into three groups: blank control group, co-culture group and DAPT co-culture group. After 10 days of co-culture, the morphological and structural changes of BMSCs were observed under light microscope. The expression of Notch signal transduction related gene Notch1, type 鈪，

本文編號(hào)：2080035