本文選題：膿毒癥休克 + 急性肺損傷��； 參考：《南京醫(yī)科大學學報(自然科學版)》2014年05期

【摘要】：目的:評價多巴胺和去甲腎上腺素對膿毒癥休克兔急性肺損傷的保護作用。方法:采用盲腸結扎穿孔(CLP)聯(lián)合靜脈注入內毒素的方法復制膿毒癥休克動物模型,將63只新西蘭大白兔分為假手術對照組(A組,n=7)、模型組(B組,n=14)、多巴胺治療組(C組,n=14)、去甲腎上腺素治療組(D組,n=14)、多巴胺聯(lián)合去甲腎上腺素治療組(E組,n=14),各組分別于造模后3、6 h處死7只。留取血清標本,用酶聯(lián)免疫吸附測定法(enzyme-linked immunosorbent assay,ELISA)檢測各時相點血清中肺泡表面活性蛋白A與肺泡表面活性蛋白D(surfactant protein A and surfactant protein D,SP-A and SP-D)的濃度值,并留取肺組織標本做光鏡和電鏡檢查。結果:各組血清中SP-A與SP-D的濃度值在各時相點均高于A組(P0.05);與B組比較,C組血清中SP-A與SP-D的濃度值在3、6 h差異均無統(tǒng)計學意義(P0.05);D、E組血清中SP-A與SP-D的濃度值在3、6 h均顯著低于B組(P0.05)。形態(tài)學觀察發(fā)現(xiàn)D、E組肺組織損傷明顯輕于B、C組。結論:膿毒癥休克時肺組織損傷嚴重;單獨應用去甲腎上腺素或聯(lián)合應用去甲腎上腺素和多巴胺可以有效減輕膿毒癥休克引起的肺組織損傷,對肺組織起保護作用。
[Abstract]:Aim: to evaluate the protective effects of dopamine and norepinephrine on acute lung injury in septic shock rabbits. Methods: septic shock model was established by cecal ligation and perforation combined with intravenous injection of endotoxin. 63 New Zealand white rabbits were divided into sham operation control group (group A), model group B (group B), dopamine group C (group C), noradrenaline group (group D), and dopamine plus norepinephrine treatment group (group E). 7 rats were killed at 6 hours after the model. Serum samples were collected and the concentrations of alveolar surfactant protein A and alveolar surfactant protein D(surfactant protein A and surfactant protein SP-A and SP-D were detected by enzyme-linked immunosorbent assaysa at different time points. The specimens of lung tissue were examined by light microscope and electron microscope. Results: the concentration of SP-A and SP-D in serum of each group was higher than that of group A at each time phase, and the concentration of SP-A and SP-D in group C was not significantly different from that in group B at 3h. There was no significant difference in the concentration of SP-A and SP-D between group A and group C at 36 h. All of them were significantly lower than group B (P 0.05). Morphological observation showed that the lung tissue injury in DU E group was significantly lighter than that in BU C group. Conclusion: the lung tissue injury is serious in septic shock and norepinephrine alone or combined with norepinephrine and dopamine can effectively attenuate the lung tissue injury induced by septic shock and play a protective role in the lung tissue.
【作者單位】： 南京醫(yī)科大學附屬南京醫(yī)院急診科;
【基金】：南京市衛(wèi)生局重大項目(2007-3-7)
【分類號】：R563.8

【參考文獻】

相關期刊論文 前1條

1 全竹富,汪義軍,劉放南,石群立,黎介壽;腹腔感染對機體器官結構和功能的影響——一種多器官功能障礙綜合征的動物模型[J];醫(yī)學研究生學報;2000年01期

【共引文獻】

相關期刊論文 前10條

1 全竹富,劉放南,陳永明,臧靜,黎介壽;腹腔感染伴多器官功能障礙綜合征時抗TNF抗體代謝調理作用的實驗研究[J];腸外與腸內營養(yǎng);2001年02期

2 黎剛;周文良;張費通;黃東健;鄺麗影;崔其亮;;新生豬多器官功能障礙綜合征模型建立的實驗研究[J];中國當代兒科雜志;2006年01期

3 林沛艾;廖婧華;;多巴胺聯(lián)合腎上腺素對43例感染性休克患者麻醉期循環(huán)功能的影響[J];福建醫(yī)藥雜志;2013年05期

4 孟繁u&;岑英文;郭應軍;黃小洵;周丹丹;;靜脈注射左西孟旦對膿毒癥休克患者心肌抑制影響的臨床療效[J];重慶醫(yī)學;2013年26期

5 劉利娟;陳怡;王偉;陳超;高明昊;張曉青;張娟琨;;肺表面活性蛋白A適配體的篩選與鑒定[J];分析化學;2013年11期

6 文強;古正濤;童華生;張興欽;蘇磊;;高分子截流持續(xù)靜脈靜脈血液濾過技術治療橫紋肌溶解癥的臨床觀察[J];廣東醫(yī)學;2013年19期

7 康U，

本文編號：1818086