研究背景:在世界范圍內子宮頸癌是一種常見的女性致死性惡性腫瘤,盡管子宮頸癌近年來的發(fā)病率和死亡率明顯下降,它依舊在女性常見腫瘤中位居第四位。microRNAs(miRNAs)是一類單鏈非編碼小RNA分子,其長度為18-24個堿基,可與靶m RNA通過堿基互補配對相結合,導致靶m RNA降解或者抑制其翻譯。在過去的十幾年中,在包括子宮頸癌在內的腫瘤領域,越來越多的研究表明miRNA扮演著一個重要調控者的角色。microRNA-501-5p(miR-501)是一種非保守的miRNA,作為腫瘤發(fā)生的潛在關鍵因子而出現(xiàn)。目前的證據表明miR-501在一些惡性腫瘤中過表達,包括肝癌,胃癌和通過促進細胞增殖作為癌細胞起作用的肺癌。此外,miR-501高表達可能是胃癌患者總體生存預測不良的指標。然而,miR-501對宮頸癌的影響和潛在機制尚未探索。CYLD基因位于染色體16q12.1上,編碼去泛素化酶。CYLD基因突變與家族性圓筒瘤(FC),多發(fā)性家族性毛發(fā)上皮瘤(MFT)和多發(fā)性骨髓瘤相關。在腎癌,HCC和子宮頸玻璃狀細胞癌細胞系中報道了CYLD的拷貝數減少。同時,在結直腸癌,乳腺癌和肺癌中發(fā)現(xiàn)抑...

【文章頁數】：89 頁

【學位級別】：碩士

【文章目錄】：
摘要
English Abstract
1 INTRODUCTION
Research justification
2 MATERIALS AND METHODS
    2.1 Materials
        2.1.1 Instruments, Equipments and Reagents
        2.1.2 Cells Line
    2.2 Methods
        2.2.1 Cell Culture
        2.2.2 Cell Transfection
        2.2.3 RNA Extraction
        2.2.4 mi R-501 detection by Taqman probe q RT-PCR
        2.2.5 SYBR Green dye method qRT-PCR detection of CYLD mRNA
        2.2.6 Western Blot analysis
        2.2.7 CCK-8 assay
        2.2.8 Colony formation assay
        2.2.9 Scratch Wound Healing assay
        2.2.10 Transwell migration and invasion assay
        2.2.11 Annexin V/Propidium iodide apoptosis assay
    2.3 Statistical analysis
3 RESULTS
    3.1 Expression of mi R-501 in Hela cells
    3.2 CYLD is downregulated by mi R-501 in cervical cancer cells
    3.3 miR-501 improves cell proliferative potential of cervical cancer via downregulating CYLD
    3.4 miR-501 promotes the migratory and invasive abilities of cervical cancer cells through downregulating CYLD
    3.5 miR-501 inhibits the apoptosis of He La cells mediated by the downregulation of CYLD
4 DISCUSSIONS
Conclusion
References
LITERATURE REVIEW
    1 Cervical Cancer and its molecular origins
    2 MicroRNA
        2.1 Biogenesis
    3 The role of micro RNA in cervical cancer
        3.1 Cell checkpoint and regulation of micro RNAs in cervical cancer
        3.2 The impact of micro RNA in the control of cells proliferation, migration, invasion and apoptosis in cervical cancer
        3.3 miRNAs associated with chemo-resistance in Cervical Cancer
        3.4 microRNA as a biomarker in cervical cancer diagnosis
        3.5 microRNA as a biomarker in cervical cancer prognosis
        3.6 Treatment with micro RNA supplementation
    4 The role of micro RNA-501 in Cancer
    SUMMARY
    REFERENCES
APPENDIX
ACKNOWLEDGMENT
List of Abbreviations
DEDICATION
PUBLICATION



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