目的:探討金雀異黃酮（Genistein,GEN）對(duì)體外培養(yǎng)的小鼠子宮內(nèi)膜異位細(xì)胞的抑制作用及其凋亡相關(guān)的機(jī)制。方法:選取30只6～7周齡,體重20g的雌性Balb/c小鼠,制備小鼠子宮內(nèi)膜異位癥動(dòng)物模型,分別從正常小鼠的子宮,異位癥小鼠子宮及異位癥小鼠的種植物中切取組織塊,降解,溫育并最終過(guò)濾獲得內(nèi)膜細(xì)胞,在DMEM培養(yǎng)基中進(jìn)行原代培養(yǎng),在電鏡下觀察其形態(tài)及生長(zhǎng)模式。不同劑量（0.1 μM,1μM,10 μM）的金雀異黃酮處理正常子宮內(nèi)膜細(xì)胞,異位癥在位細(xì)胞（EMs Eutopic cells）和異位癥異位細(xì)胞（EMs Ectopic cells）24小時(shí)后,用MTT法測(cè)定觀察GEN對(duì)內(nèi)膜細(xì)胞的抑制作用;采用ANNEXIN V/PI染色法觀察了各組內(nèi)膜細(xì)胞的凋亡率;利用PCR技術(shù)檢測(cè)高劑量（10 μM）金雀異黃酮處理前、后異位內(nèi)膜胞中Caspase-8和NF_κ B的表達(dá)。結(jié)果:1)體外成功培養(yǎng)了正常子宮內(nèi)膜、EMs在位內(nèi)膜及EMs異位內(nèi)膜細(xì)胞;電鏡下觀察異位癥內(nèi)膜間質(zhì)細(xì)胞較在位間及正常內(nèi)膜間質(zhì)細(xì)胞偏小,表面不規(guī)則,有較多微絨毛。2)正常子宮和EMs在位內(nèi)膜細(xì)胞生... 

【文章頁(yè)數(shù)】：59 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
ABSTRACT
LIST OF ABBREBRATION
CHAPTER 1-INTRODUCTION
CHAPTER 2-MATERIALS AND METHODS
    2.1 MATERIALS
        2.1.1 Animals
        2.1.2 Drugs and Reagents
        2.1.3 Instrument
    2.2 METHODS
        2.2.1 Establishing a model of endometriosis
        2.2.2 Obtaining the primary culture
        2.2.3 Cell morphology
        2.2.4 Treatment with different dose of GEN
        2.2.5 Determining cell viability by MTT Assay
        2.2.6 Quantification of apoptotic cell death by Annexin- V FITC/PI assay
        2.2.7 RNA extraction, Reverse transcription and Semi quantitative PCR
        2.2.8 Statistical Analysis
CHAPTER 3-RESULTS
    3.1 GROSS AND HISTOPATHOLOGICAL OBSERVATION
    3.2 GEN REDUCES THE ENDOMETRIOTIC CELL GROWTH IN HIGH DOSE
    3.3 GEN TRIGGERS THE APOPTOTIC CELL DEATH
    3.4 GEN AUGMENTED THE SIGNALING PATHWAYS
CHAPTER 4-DISCUSSION
CHAPTER 5-CONCLUSION
REFERENCES
ACKNOWLEDGEMENT
REVIEW ARTICLE
    References


【參考文獻(xiàn)】：
期刊論文
[1]Aberrant control of NF-κB in cancer permits transcriptional and phenotypic plasticity, to curtail dependence on host tissue: molecular mode[J]. Spiros A.Vlahopoulos.  Cancer Biology ＆ Medicine. 2017(03)
[2]Apoptosis and endometrial receptivity: Relationship with in vitro fertilization treatment outcome[J]. Yulia S Antsiferova,Natalya Y Sotnikova.  World Journal of Obstetrics and Gynecology. 2016(01)



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