目的成功的胚胎植入是正常妊娠的先決條件。胚泡植入子宮內(nèi)膜的過程涉及多種激素、細(xì)胞因子、粘附分子、免疫系統(tǒng)等的協(xié)同作用,與代謝途徑也密切相關(guān)。糖代謝是三大物質(zhì)代謝中重要組成部分,組織細(xì)胞在有氧條件下進(jìn)行糖酵解的現(xiàn)象稱為瓦氏效應(yīng)（Warburg effect）。M2型丙酮酸激酶（pyruvate kinase M2,Pkm2）是糖酵解過程中一個(gè)標(biāo)志性的代謝酶,在糖酵解過程中發(fā)揮重要作用。已有研究表明在早孕小鼠子宮內(nèi)膜蛻膜化過程中存在有氧糖酵解這一特殊現(xiàn)象,課題組前期基因芯片篩查結(jié)果初步顯示Pkm2在早孕D5小鼠子宮內(nèi)膜的表達(dá)呈現(xiàn)著床點(diǎn)高于著床旁的趨勢。但是,Pkm2在早孕小鼠子宮內(nèi)膜的表達(dá)規(guī)律、與子宮內(nèi)膜蛻膜化的關(guān)系并不清楚。因此,本研究初步探討Pkm2在早孕小鼠子宮內(nèi)膜的表達(dá)規(guī)律,為后續(xù)進(jìn)一步探討其在早孕小鼠子宮內(nèi)膜中的作用提供依據(jù)。方法（1）建立正常妊娠小鼠模型,分別收集孕D1、D4、D5、D6、D7小鼠子宮內(nèi)膜組織,采用Real-time PCR、免疫印跡法和免疫組織化學(xué)方法,檢測Pkm2在早孕小鼠子宮內(nèi)膜中的表達(dá)規(guī)律。（2）建立假孕小鼠模型,分別收集假孕PD1、PD4、PD5、PD... 

【文章來源】：重慶醫(yī)科大學(xué)重慶市

【文章頁數(shù)】：44 頁

【學(xué)位級別】：碩士

【部分圖文】：

Pkm2在早孕小鼠子宮內(nèi)膜的表達(dá)

圖 2 Pkm2 在早孕小鼠 D5、D6、D7 子宮內(nèi)膜著床點(diǎn)及著床旁的表達(dá)Fig.2 The result of relative expression of pkm2 protein and mRNA in the endometrium of miceat implantation site and inter-implantation site on D5、D6 and D7.（A-C）Real-time PCR 檢測 Pkm2 mRNA 在孕 D5、D6、D7 著床點(diǎn)及著床旁小鼠子宮內(nèi)膜表達(dá);(D-F)Western blot 檢測 Pkm2 蛋白孕 D5、D6、D7 著床點(diǎn)及著床旁小鼠子宮內(nèi)膜表達(dá);(G)D 圖的灰度分析結(jié)果;(H)E 圖的灰度分析結(jié)果;(I)F 圖的灰度分析結(jié)果。IS:著床點(diǎn)；IIS:著床旁.*P <0.05；**P <0.01；***P <0.001(A-C)Pkm2 mRNA levels determined by Real-time PCR in the endometrium of mice atimplantation site and inter-implantation site on D5 、D6 and D7; (D-F)Pkm2 protein levelsdetermined by Western blot in the endometrium of mice at implantation site andinter-implantation site on D5、D6 and D7; （G）Quantified the levels of Pkm2 protein in imageD; (H)Quantified the levels of Pkm2 protein in image E; (I)Quantified the levels of Pkm2

圖 3 Pkm2 蛋白在正常妊娠早孕小鼠子宮內(nèi)膜的表達(dá)Figure 3 The location of Pkm2 protein in the endometrium of mice during early pregnancy.D1、D4：Pkm2 蛋白在小鼠孕第 1、4 天子宮內(nèi)膜表達(dá)；D5IIS、D6IIS、D7IIS：Pkm2 蛋白在小鼠孕第 5、6、7 天著床旁子宮內(nèi)膜表達(dá)；D5IS、D6IS、D7IS：Pkm2 蛋白在小鼠孕第 5、6、7 天著床點(diǎn)子宮內(nèi)膜表達(dá)； CON：陰性對照；Le: 腔上皮；Ge: 腺上皮；S:基質(zhì)細(xì)胞；Em:胚胎；IS:著床點(diǎn)；IIS:著床旁D1、D4: Pkm2 protein location determined by IHC in the endometrium of mice during earlypregnancy; D5IIS 、 D6IIS 、 D7IIS:Pkm2 protein location determined by IHC in theendometrium of mice at inter-implantation site on D5、D6、D7;D5IS、D6IS、D7IS:Pkm2 proteinlocation determined by IHC in the endometrium of mice at implantation site on D5、D6、D7;CON: negative control; Le: luminal epithelium; Ge: gland epithelium; S: stromal cell;Em:embryo; IS:implantation site; IIS:inter-implantation site .

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期刊論文
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