目的:基于臨床樣本探討宮頸脫落細(xì)胞HPV E6/E7mR NA檢測能否作為HPV陽性女性的篩查手段。創(chuàng)新點(diǎn):首次比較了宮頸脫落細(xì)胞HPV E6/E7 mR NA檢測與宮頸脫落細(xì)胞學(xué)檢測對于HPV陽性女性宮頸活檢結(jié)果的預(yù)測價值。方法:所有入組的HPV陽性女性均進(jìn)行宮頸脫落細(xì)胞學(xué)及HPV E6/E7 mR NA檢測。比較兩種方法對于宮頸活檢結(jié)果的預(yù)測價值。結(jié)論:對于HPV陽性的女性,高級別鱗狀上皮內(nèi)病變（HSIL）HPV E6/E7 mR NA陽性率高于低級別鱗狀上皮內(nèi)病變（LSIL）及正常病例。HPV E6/E7mR NA與細(xì)胞學(xué)檢測相比,HSIL及以上級別病變的檢測敏感性（89.52%vs.86.67%,P=0.671）、特異性（48.96%vs.48.96%,P=1.000）、陽性預(yù)測值（39.00%vs.38.24%,P=1.000）及陰性預(yù)測值（92.76%vs.90.97%,P=0.678）均無顯著差異。HPV E6/E7 mR NA檢測或能成為HPV陽性女性的一種新的篩查手段。 

【文章來源】：Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2017,18(03)SCICSCD

【文章頁數(shù)】：7 頁

【文章目錄】：
1 Introduction
2 Materials and methods
    2.1 Patient recruitment and sample collection
    2.2 HPV testing
    2.3 Cytology test
    2.4 HPV E6/E7 m RNA detection
    2.5 Colposcopy and histological diagnosis
    2.6 Statistical analysis
3 Results
    3.1 Histological and cytological diagnoses
    3.2 Positive rate of HPV E6/E7 m RNA assay by histological diagnosis
    3.3 Concordance between HPV E6/E7 m RNA assay and cytological test
    3.4 Correlation of E6/E7 m RNA and cytology with histological diagnoses
4 Discussion
Compliance with ethics guidelines



本文編號：3101951