SCCE、Villin在宮頸腺癌中的表達(dá)及意義
發(fā)布時(shí)間：2019-06-04 22:51
【摘要】：目的：宮頸癌在女性惡性腫瘤中排名第二，基本分為腺癌和鱗癌，宮頸腺癌的發(fā)生率要明顯低于宮頸鱗癌，據(jù)統(tǒng)計(jì)顯示腺癌約占宮頸癌的5%-8%，但近年來(lái)，有研究表明，宮頸腺癌的發(fā)病率有所增加，由于宮頸腺癌，不容易被發(fā)現(xiàn)等原因，使宮頸腺癌的預(yù)后遠(yuǎn)遠(yuǎn)差于宮頸鱗狀細(xì)胞癌，對(duì)于宮頸腺癌做出準(zhǔn)確的病理診斷對(duì)于指導(dǎo)臨床尤為重要，本實(shí)驗(yàn)主要是研究人角化層糜蛋白酶（stratumcorneum chymotryptic enzyme, SCCE），絨毛蛋白（Villin）在宮頸腺癌發(fā)展過(guò)程的各階段中的表達(dá)情況，并分析它們對(duì)于診斷宮頸腺癌及判斷預(yù)后的重要意義。 方法：采用免疫組織化學(xué)法檢測(cè)宮頸腺癌45例、宮頸腺上皮內(nèi)瘤變（cervicalglandular intraepithelial neoplasia, CGIN）24例和正常宮頸組織24例中SCCE，Villin的表達(dá)及45例宮頸腺癌中雌激素受體（estrogen receptor，ER）、癌胚抗原（carcino-embryonic antigen，CEA）、波形蛋白（Vimentin）的表達(dá)情況，采用Western blotting方法檢測(cè)10例宮頸腺癌和5例正常宮頸組織中SCCE、Villin的表達(dá)情況，結(jié)合患者臨床病理資料和出院后的隨訪情況，統(tǒng)計(jì)學(xué)分析SPSS16.0統(tǒng)計(jì)軟件包進(jìn)行。以P0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果： 1免疫組織化學(xué)染色結(jié)果 1.1SCCE、Villin在宮頸腺癌、宮頸腺上皮內(nèi)瘤變和正常宮頸中的表達(dá) 對(duì)免疫組化結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析：SCCE、 Villin蛋白在宮頸腺癌組、CGIN組中的表達(dá)與正常宮頸組的表達(dá)比較，進(jìn)行統(tǒng)計(jì)學(xué)分析，差異具有顯著性（P0.05），宮頸腺癌組與CGIN組間比較，差異無(wú)顯著性（P＞0.05）。 1.2SCCE、Villin在宮頸腺癌中的表達(dá)情況與宮頸腺癌中ER、CEA、Vimentin表達(dá)的相關(guān)性 對(duì)免疫組化結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析：SCCE表達(dá)與Villin呈正相關(guān)（r=0.434，P0.05)、與Vimentin呈負(fù)相關(guān)（r=㧟0.373，P0.05)、與ER呈負(fù)相關(guān)（r=㧟0.629，P0.05)、與CEA表達(dá)呈正相關(guān)（r=0.434，P0.05)（見(jiàn)表3）；Villin表達(dá)與Vimentin呈負(fù)相關(guān)（r=㧟0.484，P0.05)、與ER呈負(fù)相關(guān)（r=㧟0.883，P0.05)、與CEA表達(dá)呈正相關(guān)（r=0.775，P0.05)（見(jiàn)表4）。 1.3SCCE、Villin的表達(dá)與宮頸腺癌臨床病理參數(shù)之間的關(guān)系 SCCE、Villin在宮頸腺癌I期中表達(dá)率低于II+III+IV期中的表達(dá)率，差異有顯著性，具有統(tǒng)計(jì)學(xué)意義（P0.05），在高分化宮頸腺癌中的表達(dá)率低于中、低分化宮頸腺癌中的表達(dá)率，差異有顯著性，具有統(tǒng)計(jì)學(xué)意義（P0.05），在有淋巴結(jié)轉(zhuǎn)移的宮頸腺癌中的表達(dá)率高于沒(méi)有發(fā)生淋巴結(jié)轉(zhuǎn)移患者的表達(dá)率，差異有顯著性，具有統(tǒng)計(jì)學(xué)意義（P0.05）。 1.4宮頸腺癌預(yù)后的多因素分析 Cox比例風(fēng)險(xiǎn)模型多因素分析顯示：臨床分期、組織分級(jí)、淋巴結(jié)轉(zhuǎn)移情況及SCCE的表達(dá)情況可以顯著影響宮頸腺癌患者的生存時(shí)間（P0.05），但Villin的表達(dá)情況對(duì)宮頸腺癌患者生存時(shí)間的影響不大。 1.5SCCE、Villin蛋白的表達(dá)及臨床病理參數(shù)與宮頸腺癌預(yù)后的單因素分析 臨床分期I期組，組織分級(jí)高分化組，淋巴結(jié)未發(fā)生轉(zhuǎn)移組，SCCE陰性表達(dá)組的生存率顯著高于臨床分期II+III+IV期組，組織分級(jí)中、低分化組，，淋巴結(jié)發(fā)生轉(zhuǎn)移組，SCCE陽(yáng)性表達(dá)組的生存率，并且差異有顯著性，具有統(tǒng)計(jì)學(xué)意義（P0.05）。 2Western blot結(jié)果 SCCE、Villin在宮頸腺癌組中表達(dá)率顯著高于正常宮頸組（P0.05),差異有顯著性，具有統(tǒng)計(jì)學(xué)意義。 結(jié)論 1、SCCE、Villin在正常宮頸、CGIN和宮頸腺癌組中的表達(dá)率是呈上升趨勢(shì)；在宮頸腺癌中，隨著臨床分期的增加、組織分級(jí)的降低及淋巴結(jié)轉(zhuǎn)移的發(fā)生，SCCE、Villin的表達(dá)率都是逐漸升高的，說(shuō)明SCCE及Villin的表達(dá)可能與宮頸腺癌的發(fā)生、發(fā)展及轉(zhuǎn)移有關(guān)。 2、SCCE、Villin、ER、CEA及Vimentin聯(lián)合檢測(cè)可以提高宮頸腺癌的診斷準(zhǔn)確率。 3、SCCE的表達(dá)可以作為判斷宮頸腺癌患者預(yù)后的指標(biāo)之一。
[Abstract]:Objective: Cervical cancer is the second in the female malignant tumor, which is mainly divided into adenocarcinoma and squamous cell carcinoma. The incidence of cervical adenocarcinoma is lower than that of the cervical squamous cell carcinoma. According to the statistics, it shows that the adenocarcinoma is about 5% to 8% of the cervical cancer, but in recent years, it has been shown that the incidence of cervical adenocarcinoma has increased. Because cervical adenocarcinoma is not easy to be found, the prognosis of cervical adenocarcinoma is much worse than that of the cervical squamous cell carcinoma. The expression of Villin in the development of cervical adenocarcinoma was analyzed and their significance in the diagnosis of cervical adenocarcinoma and the prognosis was also analyzed. Methods:45 cases of cervical adenocarcinoma,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue were used to detect the expression of SCCE, Villin and the expression of estrogen receptor (ER), carcinoembryonic antigen (CEA) in 45 cases of cervical adenocarcinoma. The expression of Vimentin and the expression of SCCE and Villin in 10 cases of cervical adenocarcinoma and 5 normal cervical tissues were detected by Western blotting. Line. There is a statistical meaning for the difference in the difference of P0.05 Righteousness. Results:1 immune tissue Chemical staining results 1.1 SCCE, Villin in cervical adenocarcinoma, cervical intraepithelial neoplasia and The expression of SCCE and Villin in the cervical adenocarcinoma group and the CGIN group was analyzed by the expression of SCCE and Villin in the normal cervical carcinoma. There was no significant difference between the cervical adenocarcinoma group and the CTIN group, and the difference was not significant between the cervical adenocarcinoma group and the CGIN group. (P> 0.05). 1.2 The expression of SCCE and Villin in the cervical adenocarcinoma and the ER, CEA, Vi in the cervical adenocarcinoma The correlation of mentin expression was found to be positively correlated with Villin (r = 0.434, P0.05), and negative correlation with Vimentin (r = 0.373, P0.05), and negative correlation with ER (r = 0.434, P <0.05). ? 0.629, P0.05), positive correlation with the expression of CEA (r = 0.434, P0.05) (see Table 3), negative correlation with Vimentin (r =-0.484, P0.05), negative correlation with ER (r =-0.883, P0.05), and positive correlation with the expression of CEA (r = 0.77). 5, P0.05) (see Table 4). 1.3 Expression of SCCE and Villin The relationship between the clinicopathological parameters of cervical adenocarcinoma and the expression rate of Villin in the phase I of cervical adenocarcinoma was lower than that in the II + III + IV phase, and the difference was significant (P0.05). The expression rate of the high-differentiated cervical adenocarcinoma was lower than that of the medium and the low-differentiated cervical adenocarcinoma, and the difference was significant (P0.05). The expression rate in the cervical adenocarcinoma with lymph node metastasis was higher than that of the patients without lymph node metastasis. It is of statistical significance (P 0.0). 5).4. The multi-factor analysis of the Cox proportional risk model for the prognosis of cervical adenocarcinoma showed that the clinical stage, the histological grade, the lymph node metastasis and the expression of SCCE could be displayed. The survival time of patients with cervical adenocarcinoma was affected (P0.05), but Vilin The effect of the expression of the expression on the survival time of the patients with cervical adenocarcinoma was not significant. The single-factor analysis of the expression of white and the clinicopathological parameters and the prognosis of cervical adenocarcinoma showed that the survival rate of the SCCE-negative expression group was significantly higher in the stage I, the high-differentiation group, the non-metastasis group and the SCCE-negative expression group. Bed stage II + III + IV group, tissue grade, low differentiation group, lymph node metastasis group, SCCE positive expression group's survival The rate, and the difference is significant, has a series The expression rate of SCCE and Villin in the cervical adenocarcinoma group was significantly higher than that of the Western blot. above Conclusion: The expression rate of SCCE and Villin in the normal cervical, CGIN and cervical adenocarcinoma group is on the rise. In the cervical adenocarcinoma, with the increase of clinical stage, the grade of the tissue is decreased. The expression rate of SCCE and Villin was gradually increased, and the expression rate of SCCE and Villin was gradually increased. The expression of SCCE and Villin may be related to the occurrence, development and metastasis of cervical adenocarcinoma. The combination of n, ER, CEA and Vimentin can improve the cervical adenocarcinoma
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33
[Abstract]:Objective: Cervical cancer is the second in the female malignant tumor, which is mainly divided into adenocarcinoma and squamous cell carcinoma. The incidence of cervical adenocarcinoma is lower than that of the cervical squamous cell carcinoma. According to the statistics, it shows that the adenocarcinoma is about 5% to 8% of the cervical cancer, but in recent years, it has been shown that the incidence of cervical adenocarcinoma has increased. Because cervical adenocarcinoma is not easy to be found, the prognosis of cervical adenocarcinoma is much worse than that of the cervical squamous cell carcinoma. The expression of Villin in the development of cervical adenocarcinoma was analyzed and their significance in the diagnosis of cervical adenocarcinoma and the prognosis was also analyzed. Methods:45 cases of cervical adenocarcinoma,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue,24 cases of cervical intraepithelial neoplasia (CGIN) and 24 cases of normal cervical tissue were used to detect the expression of SCCE, Villin and the expression of estrogen receptor (ER), carcinoembryonic antigen (CEA) in 45 cases of cervical adenocarcinoma. The expression of Vimentin and the expression of SCCE and Villin in 10 cases of cervical adenocarcinoma and 5 normal cervical tissues were detected by Western blotting. Line. There is a statistical meaning for the difference in the difference of P0.05 Righteousness. Results:1 immune tissue Chemical staining results 1.1 SCCE, Villin in cervical adenocarcinoma, cervical intraepithelial neoplasia and The expression of SCCE and Villin in the cervical adenocarcinoma group and the CGIN group was analyzed by the expression of SCCE and Villin in the normal cervical carcinoma. There was no significant difference between the cervical adenocarcinoma group and the CTIN group, and the difference was not significant between the cervical adenocarcinoma group and the CGIN group. (P> 0.05). 1.2 The expression of SCCE and Villin in the cervical adenocarcinoma and the ER, CEA, Vi in the cervical adenocarcinoma The correlation of mentin expression was found to be positively correlated with Villin (r = 0.434, P0.05), and negative correlation with Vimentin (r = 0.373, P0.05), and negative correlation with ER (r = 0.434, P <0.05). ? 0.629, P0.05), positive correlation with the expression of CEA (r = 0.434, P0.05) (see Table 3), negative correlation with Vimentin (r =-0.484, P0.05), negative correlation with ER (r =-0.883, P0.05), and positive correlation with the expression of CEA (r = 0.77). 5, P0.05) (see Table 4). 1.3 Expression of SCCE and Villin The relationship between the clinicopathological parameters of cervical adenocarcinoma and the expression rate of Villin in the phase I of cervical adenocarcinoma was lower than that in the II + III + IV phase, and the difference was significant (P0.05). The expression rate of the high-differentiated cervical adenocarcinoma was lower than that of the medium and the low-differentiated cervical adenocarcinoma, and the difference was significant (P0.05). The expression rate in the cervical adenocarcinoma with lymph node metastasis was higher than that of the patients without lymph node metastasis. It is of statistical significance (P 0.0). 5).4. The multi-factor analysis of the Cox proportional risk model for the prognosis of cervical adenocarcinoma showed that the clinical stage, the histological grade, the lymph node metastasis and the expression of SCCE could be displayed. The survival time of patients with cervical adenocarcinoma was affected (P0.05), but Vilin The effect of the expression of the expression on the survival time of the patients with cervical adenocarcinoma was not significant. The single-factor analysis of the expression of white and the clinicopathological parameters and the prognosis of cervical adenocarcinoma showed that the survival rate of the SCCE-negative expression group was significantly higher in the stage I, the high-differentiation group, the non-metastasis group and the SCCE-negative expression group. Bed stage II + III + IV group, tissue grade, low differentiation group, lymph node metastasis group, SCCE positive expression group's survival The rate, and the difference is significant, has a series The expression rate of SCCE and Villin in the cervical adenocarcinoma group was significantly higher than that of the Western blot. above Conclusion: The expression rate of SCCE and Villin in the normal cervical, CGIN and cervical adenocarcinoma group is on the rise. In the cervical adenocarcinoma, with the increase of clinical stage, the grade of the tissue is decreased. The expression rate of SCCE and Villin was gradually increased, and the expression rate of SCCE and Villin was gradually increased. The expression of SCCE and Villin may be related to the occurrence, development and metastasis of cervical adenocarcinoma. The combination of n, ER, CEA and Vimentin can improve the cervical adenocarcinoma
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33
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